LOCUS B399p6oN-LH 9539 bp DNA circular SYN 27-JAN-2016 DEFINITION Ligation of hpt-Xho-Mlu* into 715p7N-TE9* ACCESSION B399p6oN-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9539) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: 715p7N-TE9* Fragment ends: MluI and XhoI Fragment size: 8306 Insert molecule: hpt-Xho-Mlu* Fragment ends: XhoI and MluI Fragment size: 1233 Primer for gene synthesis hptXho CCCGCctcgagTCCTCCAtaactATGAAGAAACCGGAGC hptMlu GCCATAATACTCGAACGCGTTCACTCC Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC RBCS-TR2 CACAGTAAATTACAAGCACAAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="BL" /product="left border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..606 /gene="Pnos" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 625..1194 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 1195..1383 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 1384..1839 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1850..2140 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2316..2339 /gene="BR" /product="right border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2874..3571 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4120..7825 /gene="pVS1 ORI" /product="pVS1 ORI" /SECDrawAs="Region" /SECStyleId=1 CDS 8009..9019 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg 361 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg 421 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa 481 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg 541 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat 601 ctgcacctcg agtcctccat aactatgaag aaaccggagc tgaccgccac ttccgtcgaa 661 aagttcctca tcgagaagtt tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa 721 gagtcccgcg cgttcagctt tgatgttggc gggcgcgggt acgtcctccg cgtgaactct 781 tgcgctgacg gcttctataa agatcggtac gtttacaggc atttcgcctc ggcggccctg 841 cctattcccg aagtcctcga catcggcgag ttttccgaga gccttacgta ttgtatttcc 901 cgccgggctc aaggggtgac cctccaggat ctgccggaaa ctgagttgcc tgcggttctc 961 cagcccgtcg ccgaggctat ggacgccatc gccgcggcgg atctttctca aacgtcgggc 1021 ttcgggccgt tcggccctca ggggattggc cagtacacca cttggaggga ctttatctgc 1081 gctatcgccg atccgcacgt gtatcattgg caaaccgtta tggacgatac ggtctccgcg 1141 agcgtggccc aggctctgga cgaactcatg ttgtgggccg aggattgccc cgaggtaagt 1201 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat 1261 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt 1321 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg 1381 caggtccgcc acctggttca tgcggacttc ggctctaata acgtgctcac cgataatggg 1441 cggattactg ccgtcatcga ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg 1501 gcgaacattt tcttctggag gccttggctt gcctgcatgg aacagcaaac gcgctacttt 1561 gagcggaggc acccggagct ggcggggtct ccccgcctca gggcctatat gttgcggatc 1621 ggcctcgacc agctttacca gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg 1681 caaggccgct gtgacgctat tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc 1741 gcccggcggt cggctgccgt ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc 1801 aacaggcggc cttccacccg cccgcgggcc aaggagtgaa cgcgttcgag tattatggca 1861 ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt 1921 cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg 1981 gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg 2041 aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt 2101 ggcctctaat gaccgaagtt aatatgagga gtaaaacact gaagcctgca ggcatgcaag 2161 ctgatccact agaggccatg gcggccgcac tagtggatcc cccgggatgc atgaattcaa 2221 gcttatcgat gtcgacaggc cttaagggcc agatcttggg cccggtaccc gatcagattg 2281 tcgtttcccg ccttcggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc 2341 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat 2401 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggag tgcttggcat 2461 tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg gaaagcctga cgacggagca 2521 gcattccaaa aagatccctt ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt 2581 ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa 2641 atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt tcggccgacg tcctggccag 2701 tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc gagttgctgg atctcgcctt 2761 caatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt 2821 gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga 2881 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 2941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 3001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 3061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 3121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 3181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 3241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 3301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 3361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 3421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 3481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 3541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 3601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 3661 tggataaccg attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 3721 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac 3781 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc 3841 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc 3901 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 3961 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 4021 ccgaaacgcg cgaggcaggg gtacgtcgag gtcgatccaa cccctccgct gctatagtgc 4081 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta 4141 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag 4201 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc 4261 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca 4321 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac 4381 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt 4441 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga 4501 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac 4561 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc 4621 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt 4681 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca 4741 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta 4801 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt 4861 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga 4921 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag 4981 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc 5041 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctcgcggc ctggccggcg 5101 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa 5161 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc 5221 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga 5281 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg 5341 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa 5401 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg 5461 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg 5521 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg 5581 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct 5641 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg 5701 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca 5761 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg 5821 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa 5881 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc 5941 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc 6001 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga 6061 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta 6121 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg 6181 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg 6241 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg 6301 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt 6361 gaaggcggcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc 6421 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc 6481 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct 6541 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc 6601 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga 6661 ggtttccgca gggccggccg gcatggcgag tgtgtgggat tacgacctgg tactgatggc 6721 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg 6781 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg 6841 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat 6901 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt 6961 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga 7021 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt 7081 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg 7141 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg 7201 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga 7261 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg 7321 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct 7381 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac 7441 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa 7501 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga 7561 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc 7621 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct 7681 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat 7741 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga 7801 ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac 7861 cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc 7921 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc 7981 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac 8041 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt 8101 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg 8161 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt 8221 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg 8281 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt 8341 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct 8401 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg 8461 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc 8521 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct 8581 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc 8641 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc 8701 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga 8761 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt 8821 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc 8881 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg 8941 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac 9001 caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg 9061 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc 9121 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata 9181 tcatgaaagg ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatagc 9241 ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa tactttgcga 9301 tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca 9361 acgccatccg acggatgatg tttaaaagtc ccatgtggat cactccgttg ccccgtcgct 9421 caccgtgttg gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc 9481 ggctcagttc tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcggc //