LOCUS B402p7oN-ocs-LH 9265 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of pOCS-BM* into B400p7oN-LH* ACCESSION B402p7oN-ocs-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9265) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B400p7oN-LH* Fragment ends: SfiI Fragment size: 9001 Insert molecule: pOCS-BM* Fragment ends: SfiI Fragment size: 264 Sequencing primers SBx GGTCCTTTTGTTCATTCTCAA FEATURES Location/Qualifiers misc_feature 1..24 /gene="BL" /product="left border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..606 /gene="Pnos" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 625..858 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 859..1047 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 1048..1363 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1376..1666 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1762..1957 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2042..2065 /gene="BR" /product="right border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2600..3297 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3846..7551 /gene="pVS1 ORI" /product="pVS1 ORI" /SECDrawAs="Region" /SECStyleId=1 CDS 7735..8745 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg 361 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg 421 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa 481 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg 541 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat 601 ctgcacctcg agtcctccat aactatgggc cccgagcgca ggcccgccga catccgccgt 661 gccaccgagg ccgacatgcc agccgtctgc accatcgtga accactacat cgagacctcc 721 acggtcaact tccgcacaga gccgcaggag cctcaggagt ggaccgacga cctcgtgagg 781 ctgcgcgagc gctacccctg gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc 841 tacgcgggcc cctggaaggt aagtttctgc ttctaccttt gatatatata taataattat 901 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat 961 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat 1021 atatgaccaa aatttgttga tgtgcaggca cgcaacgcct acgactggac ggcggagagc 1081 accgtctacg tgtccccacg ccaccagcgc accggcctgg gctccacgct ctacacccac 1141 ctgctcaaga gcctggaggc ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc 1201 aacgacccga gcgtccgtat gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg 1261 gccgcgggct tcaagcacgg caactggcac gacgtgggct tctggcagct cgacttctcc 1321 ctgcctgtcc caccccgccc ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt 1381 atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt 1441 tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat 1501 gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt 1561 gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca 1621 aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacactgaag cctgcaggca 1681 tgcaagctga tccactagag gccatggcgg ccgcactagt ggatcccccg ggctgcagga 1741 attcaagctt acgcgtgtcg accctgcttt aatgagatat gcgagacgcc tatgatcgca 1801 tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag 1861 atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact atcgtatttt 1921 tatgaataat attctccgtt caatttactg attgtcctcg acaggcctta agggccagat 1981 cttgggcccg gtacccgatc agattgtcgt ttcccgcctt cggtttaaac tatcagtgtt 2041 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat 2101 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca 2161 gggttcccct cgggagtgct tggcattccg tgcgataatg acttctgttc aaccacccaa 2221 acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga tcccttggct cgtctgggtc 2281 ggctagaagg tcgagtgggc tgctgtggct tgatccctca acgcggtcgc ggacgtagcg 2341 cagcgccgaa aaatcctcga tcgcaaatcc gacgctgtcg aaaagcgtga tctgcttgtc 2401 gctctttcgg ccgacgtcct ggccagtcat cacgcgccaa agttccgtca caggatgatc 2461 tggcgcgagt tgctggatct cgccttcaat ccgggtctgt ggcgggaact ccacgaaaat 2521 atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt cgccgccgac 2581 gccgttgatg tggacgccga aaaggatcta ggtgaagatc ctttttgata atctcatgac 2641 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 2701 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 2761 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 2821 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 2881 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 2941 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 3001 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 3061 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 3121 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 3181 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 3241 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 3301 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 3361 ctttcctgcg ttatcccctg attctgtgga taaccgatta ccgcctttga gtgagctgat 3421 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 3481 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc 3541 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc 3601 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac 3661 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 3721 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcaggggtac gtcgaggtcg 3781 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct 3841 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc 3901 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg 3961 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc 4021 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag 4081 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt 4141 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc 4201 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc 4261 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc 4321 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag 4381 gccgccaagg cgcgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc 4441 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg 4501 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc 4561 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg 4621 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg 4681 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt 4741 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg 4801 atgccaagct cgcggcctgg ccggcgagct tggccgctga agaaaccgag cgccgccgtc 4861 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga 4921 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa 4981 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact 5041 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc 5101 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg 5161 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc 5221 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag 5281 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt 5341 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat 5401 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat 5461 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga 5521 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact 5581 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc 5641 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc 5701 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg 5761 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag 5821 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga 5881 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg 5941 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa 6001 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg 6061 ctgggtgatg acctggtgga gaagttgaag gcggcgcagg ccgcccagcg gcaacgcatc 6121 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa 6181 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag 6241 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc 6301 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc 6361 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggcgagtgtg 6421 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac 6481 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc 6541 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt 6601 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg 6661 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc 6721 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa 6781 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc 6841 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg 6901 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc 6961 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg 7021 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc 7081 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga 7141 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac 7201 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg 7261 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa 7321 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg 7381 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct 7441 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc 7501 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cggtgggtat 7561 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc 7621 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca 7681 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc 7741 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt 7801 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag 7861 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag 7921 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta 7981 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 8041 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat 8101 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 8161 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 8221 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 8281 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 8341 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 8401 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 8461 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 8521 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 8581 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag 8641 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt 8701 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt 8761 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata 8821 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat 8881 aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg ttatcgcaat 8941 agttggcgaa gtaatcgcaa catagcttgc ttggtcgttc cgcgtgaacg tcggctcgat 9001 tgtacctgcg ttcaaatact ttgcgatcgt gttgcgcgcc tgcccggtgc gtcggctgat 9061 ctcacggatc gactgcttct ctcgcaacgc catccgacgg atgatgttta aaagtcccat 9121 gtggatcact ccgttgcccc gtcgctcacc gtgttggggg gaaggtgcac atggctcagt 9181 tctcaatgga aattatctgc ctaaccggct cagttctgcg tagaaaccaa catgcaagct 9241 ccaccgggtg caaagcggca gcggc //