LOCUS B403p9oN-ocs-LH 9517 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of pOCS-BM* into B401p9oN-LH* ACCESSION B403p9oN-ocs-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9517) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B401p9oN-LH* Fragment ends: SfiI Fragment size: 9253 Insert molecule: pOCS-BM* Fragment ends: SfiI Fragment size: 264 Sequencing primers SBx GGTCCTTTTGTTCATTCTCAA FEATURES Location/Qualifiers misc_feature 1..24 /gene="BL" /product="left border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..606 /gene="Pnos" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 625..923 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 920..923 /label=splice /SECDrawAs="Label" CDS 924..1110 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 1109..1112 /label=splice /SECDrawAs="Label" CDS 1111..1614 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1628..1918 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2014..2209 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2294..2317 /gene="BR" /product="right border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2852..3549 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4098..7803 /gene="pVS1 ORI" /product="pVS1 ORI" /SECDrawAs="Region" /SECStyleId=1 CDS 7987..8997 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg 361 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg 421 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa 481 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg 541 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat 601 ctgcacctcg agtcctccat aactatggga gcatggattg agcaggatgg cctccatgct 661 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt 721 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg 781 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg 841 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga 901 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat 961 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag 1021 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct 1081 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac 1141 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg 1201 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact 1261 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct 1321 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc 1381 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc 1441 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg 1501 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt 1561 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttctgaacg 1621 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa 1681 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa 1741 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct 1801 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt 1861 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga 1921 agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta gtggatcccc 1981 cgggctgcag gaattcaagc ttacgcgtgt cgaccctgct ttaatgagat atgcgagacg 2041 cctatgatcg catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca 2101 tgtgtagctc agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta 2161 ctatcgtatt tttatgaata atattctccg ttcaatttac tgattgtcct cgacaggcct 2221 taagggccag atcttgggcc cggtacccga tcagattgtc gtttcccgcc ttcggtttaa 2281 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta 2341 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc 2401 atgccaacca cagggttccc ctcgggagtg cttggcattc cgtgcgataa tgacttctgt 2461 tcaaccaccc aaacgtcgga aagcctgacg acggagcagc attccaaaaa gatcccttgg 2521 ctcgtctggg tcggctagaa ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc 2581 gcggacgtag cgcagcgccg aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt 2641 gatctgcttg tcgctctttc ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt 2701 cacaggatga tctggcgcga gttgctggat ctcgccttca atccgggtct gtggcgggaa 2761 ctccacgaaa atatccgaac gcagcaagat atcgcggtgc atctcggtct tgcctgggca 2821 gtcgccgccg acgccgttga tgtggacgcc gaaaaggatc taggtgaaga tcctttttga 2881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 2941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 3001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 3061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 3121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 3181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 3241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 3301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 3361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 3421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 3481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 3541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 3601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgat taccgccttt 3661 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 3721 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 3781 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca 3841 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg 3901 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 3961 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcaggggt 4021 acgtcgaggt cgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg 4081 cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc 4141 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc 4201 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat 4261 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc 4321 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg 4381 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc 4441 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg 4501 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg 4561 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg 4621 agcgggcgcg aggccgccaa ggcgcgaggc gtgaagtttg gcccccgccc taccctcacc 4681 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag 4741 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag 4801 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag 4861 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc 4921 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg 4981 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg 5041 ccggtttgtc tgatgccaag ctcgcggcct ggccggcgag cttggccgct gaagaaaccg 5101 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg 5161 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat 5221 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg 5281 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc 5341 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc 5401 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc 5461 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc 5521 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca 5581 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa 5641 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct 5701 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt 5761 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat 5821 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg 5881 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca 5941 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg 6001 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg 6061 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg 6121 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg 6181 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac 6241 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa 6301 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggcggcgca ggccgcccag 6361 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga 6421 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc 6481 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat 6541 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc 6601 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc 6661 atggcgagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc 6721 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt 6781 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta 6841 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag 6901 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta 6961 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc 7021 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc 7081 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa 7141 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag 7201 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag 7261 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc 7321 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg 7381 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg 7441 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca 7501 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta 7561 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca 7621 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct 7681 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat 7741 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc 7801 accggtgggt atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag 7861 gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa 7921 cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag 7981 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc 8041 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg 8101 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac 8161 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg 8221 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa 8281 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac 8341 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct 8401 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct 8461 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc 8521 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc 8581 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg 8641 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg 8701 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat 8761 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag 8821 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt 8881 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag 8941 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt 9001 ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc 9061 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta 9121 agcgtgcata ataagcccta cacaaattgg gagatatatc atgaaaggct ggctttttct 9181 tgttatcgca atagttggcg aagtaatcgc aacatagctt gcttggtcgt tccgcgtgaa 9241 cgtcggctcg attgtacctg cgttcaaata ctttgcgatc gtgttgcgcg cctgcccggt 9301 gcgtcggctg atctcacgga tcgactgctt ctctcgcaac gccatccgac ggatgatgtt 9361 taaaagtccc atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc 9421 acatggctca gttctcaatg gaaattatct gcctaaccgg ctcagttctg cgtagaaacc 9481 aacatgcaag ctccaccggg tgcaaagcgg cagcggc //