LOCUS B422p6oN-ocs-LH 9739 bp DNA circular SYN 21-JAN-2016 DEFINITION Ligation of pOCS-BM* into B399p6oN-LH* ACCESSION B422p6oN-ocs-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9739) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B399p6oN-LH* Fragment ends: SfiI Fragment size: 9475 Insert molecule: pOCS-BM* Fragment ends: SfiI Fragment size: 264 Sequencing primers SphR GGGTGGTTGAACAGAAGTCAT auch Maxi FEATURES Location/Qualifiers misc_feature 1..24 /gene="BL" /product="left border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..606 /gene="Pnos" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 625..1194 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 1195..1383 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 1384..1839 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1850..2140 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2236..2431 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2516..2539 /gene="BR" /product="right border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3074..3771 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4320..8025 /gene="pVS1 ORI" /product="pVS1 ORI" /SECDrawAs="Region" /SECStyleId=1 CDS 8209..9219 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg 361 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg 421 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa 481 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg 541 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat 601 ctgcacctcg agtcctccat aactatgaag aaaccggagc tgaccgccac ttccgtcgaa 661 aagttcctca tcgagaagtt tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa 721 gagtcccgcg cgttcagctt tgatgttggc gggcgcgggt acgtcctccg cgtgaactct 781 tgcgctgacg gcttctataa agatcggtac gtttacaggc atttcgcctc ggcggccctg 841 cctattcccg aagtcctcga catcggcgag ttttccgaga gccttacgta ttgtatttcc 901 cgccgggctc aaggggtgac cctccaggat ctgccggaaa ctgagttgcc tgcggttctc 961 cagcccgtcg ccgaggctat ggacgccatc gccgcggcgg atctttctca aacgtcgggc 1021 ttcgggccgt tcggccctca ggggattggc cagtacacca cttggaggga ctttatctgc 1081 gctatcgccg atccgcacgt gtatcattgg caaaccgtta tggacgatac ggtctccgcg 1141 agcgtggccc aggctctgga cgaactcatg ttgtgggccg aggattgccc cgaggtaagt 1201 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat 1261 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt 1321 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg 1381 caggtccgcc acctggttca tgcggacttc ggctctaata acgtgctcac cgataatggg 1441 cggattactg ccgtcatcga ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg 1501 gcgaacattt tcttctggag gccttggctt gcctgcatgg aacagcaaac gcgctacttt 1561 gagcggaggc acccggagct ggcggggtct ccccgcctca gggcctatat gttgcggatc 1621 ggcctcgacc agctttacca gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg 1681 caaggccgct gtgacgctat tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc 1741 gcccggcggt cggctgccgt ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc 1801 aacaggcggc cttccacccg cccgcgggcc aaggagtgaa cgcgttcgag tattatggca 1861 ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt 1921 cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg 1981 gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg 2041 aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt 2101 ggcctctaat gaccgaagtt aatatgagga gtaaaacact gaagcctgca ggcatgcaag 2161 ctgatccact agaggccatg gcggccgcac tagtggatcc cccgggctgc aggaattcaa 2221 gcttacgcgt gtcgaccctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat 2281 ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt 2341 accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa 2401 taatattctc cgttcaattt actgattgtc ctcgacaggc cttaagggcc agatcttggg 2461 cccggtaccc gatcagattg tcgtttcccg ccttcggttt aaactatcag tgtttgacag 2521 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa 2581 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc 2641 ccctcgggag tgcttggcat tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg 2701 gaaagcctga cgacggagca gcattccaaa aagatccctt ggctcgtctg ggtcggctag 2761 aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc 2821 cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt 2881 tcggccgacg tcctggccag tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc 2941 gagttgctgg atctcgcctt caatccgggt ctgtggcggg aactccacga aaatatccga 3001 acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt 3061 gatgtggacg ccgaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 3121 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 3181 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 3241 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 3301 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 3361 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 3421 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 3481 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 3541 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 3601 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 3661 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 3721 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 3781 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 3841 tgcgttatcc cctgattctg tggataaccg attaccgcct ttgagtgagc tgataccgct 3901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg 3961 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc 4021 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg 4081 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 4141 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 4201 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg gtacgtcgag gtcgatccaa 4261 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac 4321 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt 4381 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat 4441 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac 4501 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt 4561 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac 4621 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc 4681 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca 4741 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc 4801 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc 4861 aaggcgcgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac 4921 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc 4981 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag 5041 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc 5101 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac 5161 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc 5221 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca 5281 agctcgcggc ctggccggcg agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa 5341 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat 5401 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa 5461 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg 5521 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt 5581 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt 5641 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt 5701 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta 5761 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga 5821 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg 5881 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca 5941 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga 6001 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg 6061 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg 6121 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag 6181 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc 6241 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg 6301 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca 6361 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc 6421 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga 6481 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt 6541 gatgacctgg tggagaagtt gaaggcggcg caggccgccc agcggcaacg catcgaggca 6601 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg 6661 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca 6721 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac 6781 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag 6841 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggcgag tgtgtgggat 6901 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa 6961 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc 7021 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta 7081 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg 7141 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg 7201 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag 7261 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt 7321 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag 7381 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc 7441 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct 7501 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc 7561 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt 7621 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac 7681 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat 7741 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt 7801 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa 7861 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg 7921 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa 7981 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga 8041 cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact 8101 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc 8161 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc 8221 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg 8281 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg 8341 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc 8401 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact 8461 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta 8521 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg 8581 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg 8641 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt 8701 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa 8761 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg 8821 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag 8881 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg 8941 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc 9001 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg 9061 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat 9121 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac 9181 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc 9241 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct 9301 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc 9361 tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg 9421 cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc 9481 tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg 9541 gatcgactgc ttctctcgca acgccatccg acggatgatg tttaaaagtc ccatgtggat 9601 cactccgttg ccccgtcgct caccgtgttg gggggaaggt gcacatggct cagttctcaa 9661 tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa ccaacatgca agctccaccg 9721 ggtgcaaagc ggcagcggc //