LOCUS B415p9o-LH 9437 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of B401p9oN-LH* into pLH7000M* ACCESSION B415p9o-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9437) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pLH7000M* Fragment ends: PstI and XhoI Fragment size: 8116 Insert molecule: B401p9oN-LH* Fragment ends: XhoI and PstI Fragment size: 1321 Sequencing primers 35S GCACAATCCCACTATCCTT LH1.2 CGAAGGCGGGAAACGACAAT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..1043 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 1040..1043 /label=splice /SECDrawAs="Label" CDS 1044..1230 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 1229..1232 /label=splice /SECDrawAs="Label" CDS 1231..1734 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1748..2038 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2214..2237 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2772..3469 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3506..3765 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4018..7723 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 7907..8917 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccat aactatggga gcatggattg agcaggatgg cctccatgct 781 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt 841 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg 901 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg 961 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga 1021 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat 1081 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag 1141 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct 1201 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac 1261 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg 1321 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact 1381 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct 1441 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc 1501 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc 1561 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg 1621 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt 1681 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttctgaacg 1741 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa 1801 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa 1861 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct 1921 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt 1981 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga 2041 agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta gtggatcccc 2101 cgggatgcat gaattcaagc ttatcgatgt cgacaggcct taagggccag atcttgggcc 2161 cggtacccga tcagattgtc gtttcccgcc ttcggtttaa actatcagtg tttgacagga 2221 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa 2281 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc 2341 ctcgggagtg cttggcattc cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga 2401 aagcctgacg acggagcagc attccaaaaa gatcccttgg ctcgtctggg tcggctagaa 2461 ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc gcggacgtag cgcagcgccg 2521 aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc 2581 ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt cacaggatga tctggcgcga 2641 gttgctggat ctcgccttca atccgggtct gtggcgggaa ctccacgaaa atatccgaac 2701 gcagcaagat atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga 2761 tgtggacgcc gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 2821 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 2881 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 2941 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 3001 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 3061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 3121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 3181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 3241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 3301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 3361 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 3421 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 3481 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 3541 cgttatcccc tgattctgtg gataaccgat taccgccttt gagtgagctg ataccgctcg 3601 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat 3661 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag 3721 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac 3781 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 3841 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 3901 aggttttcac cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc 3961 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga 4021 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt 4081 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta 4141 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga 4201 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc 4261 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct 4321 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga 4381 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga 4441 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat 4501 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa 4561 ggcgcgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc 4621 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt 4681 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc 4741 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc 4801 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg 4861 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg 4921 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag 4981 ctcgcggcct ggccggcgag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg 5041 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga 5101 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag 5161 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg 5221 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc 5281 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga 5341 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg 5401 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg 5461 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg 5521 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg 5581 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc 5641 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg 5701 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag 5761 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag 5821 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg 5881 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca 5941 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag 6001 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag 6061 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag 6121 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg 6181 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga 6241 tgacctggtg gagaagttga aggcggcgca ggccgcccag cggcaacgca tcgaggcaga 6301 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca 6361 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga 6421 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt 6481 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct 6541 tccagacggg cacgtagagg tttccgcagg gccggccggc atggcgagtg tgtgggatta 6601 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg 6661 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg 6721 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa 6781 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt 6841 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc 6901 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa 6961 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt 7021 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac 7081 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa 7141 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg 7201 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta 7261 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct 7321 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc 7381 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat 7441 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa 7501 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc 7561 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc 7621 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc 7681 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accggtgggt atgcctgacg 7741 atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc 7801 gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca 7861 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa 7921 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc 7981 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg 8041 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag 8101 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca 8161 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca 8221 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt 8281 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga 8341 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt 8401 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg 8461 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc 8521 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga 8581 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct 8641 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg 8701 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc 8761 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct 8821 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta 8881 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg 8941 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca 9001 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta 9061 cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg 9121 aagtaatcgc aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg 9181 cgttcaaata ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga 9241 tcgactgctt ctctcgcaac gccatccgac ggatgatgtt taaaagtccc atgtggatca 9301 ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc acatggctca gttctcaatg 9361 gaaattatct gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag ctccaccggg 9421 tgcaaagcgg cagcggc //