LOCUS B423p6o-LH-35s-o 10287 bp DNA circular SYN 21-JAN-2016 DEFINITION Ligation of p35-OCS-BM* into B413p6o-LH* ACCESSION B423p6o-LH-35s-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10287) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B413p6o-LH* Fragment ends: SfiI Fragment size: 9595 Insert molecule: p35-OCS-BM* Fragment ends: SfiI Fragment size: 692 Sequencing primers E9F GTGTCAAATCGTGGCCTCTAA FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..1314 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 1315..1503 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 1504..1959 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1970..2260 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2315..2737 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" misc_feature 2784..2979 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3064..3087 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3622..4319 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4356..4615 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4868..8573 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8757..9767 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccat aactatgaag aaaccggagc tgaccgccac ttccgtcgaa 781 aagttcctca tcgagaagtt tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa 841 gagtcccgcg cgttcagctt tgatgttggc gggcgcgggt acgtcctccg cgtgaactct 901 tgcgctgacg gcttctataa agatcggtac gtttacaggc atttcgcctc ggcggccctg 961 cctattcccg aagtcctcga catcggcgag ttttccgaga gccttacgta ttgtatttcc 1021 cgccgggctc aaggggtgac cctccaggat ctgccggaaa ctgagttgcc tgcggttctc 1081 cagcccgtcg ccgaggctat ggacgccatc gccgcggcgg atctttctca aacgtcgggc 1141 ttcgggccgt tcggccctca ggggattggc cagtacacca cttggaggga ctttatctgc 1201 gctatcgccg atccgcacgt gtatcattgg caaaccgtta tggacgatac ggtctccgcg 1261 agcgtggccc aggctctgga cgaactcatg ttgtgggccg aggattgccc cgaggtaagt 1321 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat 1381 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt 1441 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg 1501 caggtccgcc acctggttca tgcggacttc ggctctaata acgtgctcac cgataatggg 1561 cggattactg ccgtcatcga ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg 1621 gcgaacattt tcttctggag gccttggctt gcctgcatgg aacagcaaac gcgctacttt 1681 gagcggaggc acccggagct ggcggggtct ccccgcctca gggcctatat gttgcggatc 1741 ggcctcgacc agctttacca gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg 1801 caaggccgct gtgacgctat tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc 1861 gcccggcggt cggctgccgt ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc 1921 aacaggcggc cttccacccg cccgcgggcc aaggagtgaa cgcgttcgag tattatggca 1981 ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt 2041 cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg 2101 gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg 2161 aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt 2221 ggcctctaat gaccgaagtt aatatgagga gtaaaacact gaagcctgca ggcatgcaag 2281 ctgatccact agaggccatg gcggccgcac tagatcaaca tggtggagca cgacactctc 2341 gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat tgagactttt 2401 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc 2461 atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg cgataaagga 2521 aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc cccacccacg 2581 aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt 2641 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc 2701 tctatataag gaagttcatt tcatttggag aggacactag ttggatcccc cgggctgcag 2761 gaattcaagc ttacgcgtgt cgaccctgct ttaatgagat atgcgagacg cctatgatcg 2821 catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc 2881 agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt 2941 tttatgaata atattctccg ttcaatttac tgattgtcct cgacaggcct taagggccag 3001 atcttgggcc cggtacccga tcagattgtc gtttcccgcc ttcggtttaa actatcagtg 3061 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg 3121 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca 3181 cagggttccc ctcgggagtg cttggcattc cgtgcgataa tgacttctgt tcaaccaccc 3241 aaacgtcgga aagcctgacg acggagcagc attccaaaaa gatcccttgg ctcgtctggg 3301 tcggctagaa ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc gcggacgtag 3361 cgcagcgccg aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg 3421 tcgctctttc ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt cacaggatga 3481 tctggcgcga gttgctggat ctcgccttca atccgggtct gtggcgggaa ctccacgaaa 3541 atatccgaac gcagcaagat atcgcggtgc atctcggtct tgcctgggca gtcgccgccg 3601 acgccgttga tgtggacgcc gaaaaggatc taggtgaaga tcctttttga taatctcatg 3661 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 3721 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 3781 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 3841 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 3901 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 3961 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 4021 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 4081 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 4141 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 4201 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 4261 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 4321 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 4381 ttctttcctg cgttatcccc tgattctgtg gataaccgat taccgccttt gagtgagctg 4441 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 4501 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt 4561 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc 4621 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg 4681 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 4741 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt 4801 cgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt 4861 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt 4921 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc 4981 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca 5041 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca 5101 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc 5161 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca 5221 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta 5281 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt 5341 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg 5401 aggccgccaa ggcgcgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga 5461 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac 5521 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc 5581 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc 5641 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca 5701 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt 5761 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc 5821 tgatgccaag ctcgcggcct ggccggcgag cttggccgct gaagaaaccg agcgccgccg 5881 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat 5941 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt 6001 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa 6061 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg 6121 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac 6181 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg 6241 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca 6301 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag 6361 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc 6421 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt 6481 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca 6541 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa 6601 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg 6661 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag 6721 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg 6781 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag 6841 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg 6901 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc 6961 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc 7021 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg 7081 cgctgggtga tgacctggtg gagaagttga aggcggcgca ggccgcccag cggcaacgca 7141 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag 7201 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg 7261 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca 7321 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc 7381 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggcgagtg 7441 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat 7501 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac 7561 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca 7621 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc 7681 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa 7741 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag 7801 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca 7861 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt 7921 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca 7981 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg 8041 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg 8101 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc 8161 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga 8221 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag 8281 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta 8341 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc 8401 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc 8461 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc 8521 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accggtgggt 8581 atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg 8641 cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg 8701 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc 8761 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg 8821 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc 8881 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc 8941 agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag 9001 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc 9061 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg 9121 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg 9181 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca 9241 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat 9301 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca 9361 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag 9421 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa 9481 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta 9541 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg 9601 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac 9661 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat 9721 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc 9781 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca 9841 taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata 9901 ataagcccta cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca 9961 atagttggcg aagtaatcgc aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg 10021 attgtacctg cgttcaaata ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg 10081 atctcacgga tcgactgctt ctctcgcaac gccatccgac ggatgatgtt taaaagtccc 10141 atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc acatggctca 10201 gttctcaatg gaaattatct gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag 10261 ctccaccggg tgcaaagcgg cagcggc //