LOCUS B424p7o-LH-35s-o 9813 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of p35-OCS-BM* into B414p7o-LH* ACCESSION B424p7o-LH-35s-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9813) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B414p7o-LH* Fragment ends: SfiI Fragment size: 9121 Insert molecule: p35-OCS-BM* Fragment ends: SfiI Fragment size: 692 Sequencing primers E9F GTGTCAAATCGTGGCCTCTAA FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..978 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 979..1167 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 1168..1483 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1496..1786 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1841..2263 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" misc_feature 2310..2505 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2590..2613 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3148..3845 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3882..4141 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4394..8099 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8283..9293 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccat aactatgggc cccgagcgca ggcccgccga catccgccgt 781 gccaccgagg ccgacatgcc agccgtctgc accatcgtga accactacat cgagacctcc 841 acggtcaact tccgcacaga gccgcaggag cctcaggagt ggaccgacga cctcgtgagg 901 ctgcgcgagc gctacccctg gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc 961 tacgcgggcc cctggaaggt aagtttctgc ttctaccttt gatatatata taataattat 1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat 1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat 1141 atatgaccaa aatttgttga tgtgcaggca cgcaacgcct acgactggac ggcggagagc 1201 accgtctacg tgtccccacg ccaccagcgc accggcctgg gctccacgct ctacacccac 1261 ctgctcaaga gcctggaggc ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc 1321 aacgacccga gcgtccgtat gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg 1381 gccgcgggct tcaagcacgg caactggcac gacgtgggct tctggcagct cgacttctcc 1441 ctgcctgtcc caccccgccc ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt 1501 atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt 1561 tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat 1621 gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt 1681 gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca 1741 aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacactgaag cctgcaggca 1801 tgcaagctga tccactagag gccatggcgg ccgcactaga tcaacatggt ggagcacgac 1861 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag 1921 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt 1981 cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat 2041 aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca 2101 cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 2161 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac 2221 ccttcctcta tataaggaag ttcatttcat ttggagagga cactagttgg atcccccggg 2281 ctgcaggaat tcaagcttac gcgtgtcgac cctgctttaa tgagatatgc gagacgccta 2341 tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg 2401 tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat 2461 cgtattttta tgaataatat tctccgttca atttactgat tgtcctcgac aggccttaag 2521 ggccagatct tgggcccggt acccgatcag attgtcgttt cccgccttcg gtttaaacta 2581 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat 2641 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc 2701 caaccacagg gttcccctcg ggagtgcttg gcattccgtg cgataatgac ttctgttcaa 2761 ccacccaaac gtcggaaagc ctgacgacgg agcagcattc caaaaagatc ccttggctcg 2821 tctgggtcgg ctagaaggtc gagtgggctg ctgtggcttg atccctcaac gcggtcgcgg 2881 acgtagcgca gcgccgaaaa atcctcgatc gcaaatccga cgctgtcgaa aagcgtgatc 2941 tgcttgtcgc tctttcggcc gacgtcctgg ccagtcatca cgcgccaaag ttccgtcaca 3001 ggatgatctg gcgcgagttg ctggatctcg ccttcaatcc gggtctgtgg cgggaactcc 3061 acgaaaatat ccgaacgcag caagatatcg cggtgcatct cggtcttgcc tgggcagtcg 3121 ccgccgacgc cgttgatgtg gacgccgaaa aggatctagg tgaagatcct ttttgataat 3181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 3241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 3301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 3361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 3421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 3481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 3541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 3601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 3661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 3721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 3781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 3841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 3901 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgattacc gcctttgagt 3961 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 4021 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 4081 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 4141 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 4201 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 4261 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc aggggtacgt 4321 cgaggtcgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc 4381 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc 4441 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact 4501 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc 4561 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct 4621 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca 4681 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg 4741 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc 4801 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga 4861 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg 4921 ggcgcgaggc cgccaaggcg cgaggcgtga agtttggccc ccgccctacc ctcaccccgg 4981 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg 5041 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag 5101 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg 5161 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga 5221 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg 5281 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg 5341 tttgtctgat gccaagctcg cggcctggcc ggcgagcttg gccgctgaag aaaccgagcg 5401 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc 5461 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct 5521 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc 5581 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc 5641 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg 5701 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc 5761 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg 5821 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc 5881 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc 5941 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag 6001 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt 6061 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa 6121 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa 6181 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca 6241 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga 6301 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc 6361 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc 6421 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga 6481 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga 6541 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg 6601 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc ggcgcaggcc gcccagcggc 6661 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc 6721 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg 6781 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc 6841 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg 6901 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg 6961 cgagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga 7021 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg 7081 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa 7141 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg 7201 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga 7261 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga 7321 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc 7381 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca 7441 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct 7501 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg 7561 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag 7621 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa 7681 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca 7741 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca 7801 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac 7861 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg 7921 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc 7981 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac 8041 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccg 8101 gtgggtatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca 8161 gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga 8221 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc 8281 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag 8341 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat 8401 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg 8461 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg 8521 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga 8581 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg 8641 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga 8701 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag 8761 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac 8821 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga 8881 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag 8941 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa 9001 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag 9061 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg 9121 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag 9181 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg 9241 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa 9301 caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta 9361 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg 9421 tgcataataa gccctacaca aattgggaga tatatcatga aaggctggct ttttcttgtt 9481 atcgcaatag ttggcgaagt aatcgcaaca tagcttgctt ggtcgttccg cgtgaacgtc 9541 ggctcgattg tacctgcgtt caaatacttt gcgatcgtgt tgcgcgcctg cccggtgcgt 9601 cggctgatct cacggatcga ctgcttctct cgcaacgcca tccgacggat gatgtttaaa 9661 agtcccatgt ggatcactcc gttgccccgt cgctcaccgt gttgggggga aggtgcacat 9721 ggctcagttc tcaatggaaa ttatctgcct aaccggctca gttctgcgta gaaaccaaca 9781 tgcaagctcc accgggtgca aagcggcagc ggc //