LOCUS B425p9o-LH-35s-o 10065 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of p35-OCS-BM* into B415p9o-LH* ACCESSION B425p9o-LH-35s-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10065) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B415p9o-LH* Fragment ends: SfiI Fragment size: 9373 Insert molecule: p35-OCS-BM* Fragment ends: SfiI Fragment size: 692 Sequencing primers E9F GTGTCAAATCGTGGCCTCTAA FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..1043 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 1040..1043 /label=splice /SECDrawAs="Label" CDS 1044..1230 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 1229..1232 /label=splice /SECDrawAs="Label" CDS 1231..1734 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1748..2038 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2093..2515 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" misc_feature 2562..2757 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2842..2865 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3400..4097 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4134..4393 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4646..8351 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8535..9545 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccat aactatggga gcatggattg agcaggatgg cctccatgct 781 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt 841 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg 901 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg 961 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga 1021 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat 1081 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag 1141 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct 1201 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac 1261 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg 1321 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact 1381 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct 1441 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc 1501 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc 1561 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg 1621 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt 1681 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttctgaacg 1741 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa 1801 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa 1861 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct 1921 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt 1981 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga 2041 agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta gatcaacatg 2101 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccag 2161 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc 2221 ccagctatct gtcacttcat cgaaaggaca gtagaaaagg aagatggctt ctacaaatgc 2281 catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctaccgacag tggtcccaaa 2341 gatggacccc cacccacgag gaacatcgtg gaaaaagaag acgttccaac cacgtcttca 2401 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat 2461 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacactagtt 2521 ggatcccccg ggctgcagga attcaagctt acgcgtgtcg accctgcttt aatgagatat 2581 gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa 2641 cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc 2701 acccgttact atcgtatttt tatgaataat attctccgtt caatttactg attgtcctcg 2761 acaggcctta agggccagat cttgggcccg gtacccgatc agattgtcgt ttcccgcctt 2821 cggtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc 2881 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt 2941 gtatgtgcat gccaaccaca gggttcccct cgggagtgct tggcattccg tgcgataatg 3001 acttctgttc aaccacccaa acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga 3061 tcccttggct cgtctgggtc ggctagaagg tcgagtgggc tgctgtggct tgatccctca 3121 acgcggtcgc ggacgtagcg cagcgccgaa aaatcctcga tcgcaaatcc gacgctgtcg 3181 aaaagcgtga tctgcttgtc gctctttcgg ccgacgtcct ggccagtcat cacgcgccaa 3241 agttccgtca caggatgatc tggcgcgagt tgctggatct cgccttcaat ccgggtctgt 3301 ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtgcat ctcggtcttg 3361 cctgggcagt cgccgccgac gccgttgatg tggacgccga aaaggatcta ggtgaagatc 3421 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3481 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3541 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3601 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 3661 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3721 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3781 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3841 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 3901 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 3961 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 4021 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 4081 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 4141 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgatta 4201 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 4261 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 4321 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 4381 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac 4441 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 4501 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 4561 gcaggggtac gtcgaggtcg atccaacccc tccgctgcta tagtgcagtc ggcttctgac 4621 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg 4681 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga 4741 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc 4801 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc 4861 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc 4921 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc 4981 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg 5041 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc 5101 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc 5161 gcacccggag cgggcgcgag gccgccaagg cgcgaggcgt gaagtttggc ccccgcccta 5221 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg 5281 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc 5341 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat 5401 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa 5461 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat 5521 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga 5581 aatcctggcc ggtttgtctg atgccaagct cgcggcctgg ccggcgagct tggccgctga 5641 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca 5701 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg 5761 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat 5821 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag 5881 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc 5941 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc 6001 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg 6061 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg 6121 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg 6181 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg 6241 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc 6301 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc 6361 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca 6421 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca 6481 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca 6541 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat 6601 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg 6661 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc 6721 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc 6781 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc 6841 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gcggcgcagg 6901 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg 6961 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga 7021 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca 7081 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac 7141 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc 7201 cggccggcat ggcgagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa 7261 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc 7321 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg 7381 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga 7441 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca 7501 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga 7561 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact 7621 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca 7681 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt 7741 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg 7801 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga 7861 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc 7921 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc 7981 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga 8041 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa 8101 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc 8161 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc 8221 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc 8281 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac 8341 atcaaggcac cggtgggtat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg 8401 ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac 8461 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt 8521 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg 8581 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta 8641 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga 8701 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg 8761 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc 8821 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga 8881 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc 8941 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc 9001 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag 9061 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg 9121 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg 9181 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat 9241 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg 9301 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg 9361 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg 9421 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg 9481 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca 9541 aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg 9601 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat 9661 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg 9721 ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa catagcttgc ttggtcgttc 9781 cgcgtgaacg tcggctcgat tgtacctgcg ttcaaatact ttgcgatcgt gttgcgcgcc 9841 tgcccggtgc gtcggctgat ctcacggatc gactgcttct ctcgcaacgc catccgacgg 9901 atgatgttta aaagtcccat gtggatcact ccgttgcccc gtcgctcacc gtgttggggg 9961 gaaggtgcac atggctcagt tctcaatgga aattatctgc ctaaccggct cagttctgcg 10021 tagaaaccaa catgcaagct ccaccgggtg caaagcggca gcggc //