LOCUS B708p7o-LH-LGFP 10352 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of B636pLGFP-ocs* into B414p7o-LH* ACCESSION B708p7o-LH-LGFP KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10352) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B414p7o-LH* Fragment ends: SfiI Fragment size: 9121 Insert molecule: B636pLGFP-ocs* Fragment ends: SfiI Fragment size: 1231 Sequencing primers GFP-seq CCAGTTCCACCAGGATTG OF2 CCGGTTTCGGTTCATTCTAA FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..978 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 979..1167 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 1168..1483 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1496..1786 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_signal 1883..1933 /label=link /SECDrawAs="Label" CDS 1934..2312 /gene="gfpm" /product="GFPm" /SECDrawAs="Gene" /SECStyleId=1 CDS 2313..2501 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 2502..2839 /gene="gfpm" /product="GFPm" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2849..3044 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3129..3152 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3687..4384 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4421..4680 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4933..8638 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8822..9832 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccat aactatgggc cccgagcgca ggcccgccga catccgccgt 781 gccaccgagg ccgacatgcc agccgtctgc accatcgtga accactacat cgagacctcc 841 acggtcaact tccgcacaga gccgcaggag cctcaggagt ggaccgacga cctcgtgagg 901 ctgcgcgagc gctacccctg gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc 961 tacgcgggcc cctggaaggt aagtttctgc ttctaccttt gatatatata taataattat 1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat 1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat 1141 atatgaccaa aatttgttga tgtgcaggca cgcaacgcct acgactggac ggcggagagc 1201 accgtctacg tgtccccacg ccaccagcgc accggcctgg gctccacgct ctacacccac 1261 ctgctcaaga gcctggaggc ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc 1321 aacgacccga gcgtccgtat gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg 1381 gccgcgggct tcaagcacgg caactggcac gacgtgggct tctggcagct cgacttctcc 1441 ctgcctgtcc caccccgccc ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt 1501 atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt 1561 tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat 1621 gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt 1681 gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca 1741 aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacactgaag cctgcaggca 1801 tgcaagctga tccactagag gccatggcgg ccgcactagt ggatcccccg ggctgcagga 1861 attcaagctt acgcgttgta caggagtacc gccccgtccg gtcctgcccg tcaccgagat 1921 cagcggagag ttcatgggca agggcgagga actgttcact ggcgtggtcc caatcctggt 1981 ggaactggat ggtgatgtga acgggcacaa gttctccgtc agcggagagg gtgaaggtga 2041 tgccacctac ggaaagctca ccctgaagtt catctgcact accggaaagc tccctgttcc 2101 gtggccaacc ctcgtcacca ctttcaccta cggtgttcag tgcttctccc gttacccaga 2161 tcacatgaag cagcatgact tcttcaagag cgccatgccc gaaggctacg tgcaagaaag 2221 gactatcttc ttcaaggatg acgggaacta caagacacgt gccgaagtca agttcgaagg 2281 tgataccctg gtgaaccgca tcgagctgaa aggtaagttt ctgcttctac ctttgatata 2341 tatataataa ttatcattaa ttagtagtaa tataatattt caaatatttt tttcaaaata 2401 aaagaatgta gtatatagca attgcttttc tgtagtttat aagtgtgtat attttaattt 2461 ataacttttc taatatatga ccaaaatttg ttgatgtgca ggtatcgatt tcaaggaaga 2521 tggaaacatc ctcggacaca agctggagta caactacaac tcccacaacg tatacatcat 2581 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atcaggcaca acatcgaaga 2641 tggaagcgtg caactggcgg accactacca gcagaacacg cccatcggcg atggccctgt 2701 cctgctgccg gacaaccatt acctgtccac gcaatctgcc ctctccaagg accccaacga 2761 gaagagggac cacatggtcc tgctggagtt cgtgacggct gctgggatca cgcatggcat 2821 ggatgaactc tacaagtgac ccggtcgacc ctgctttaat gagatatgcg agacgcctat 2881 gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt 2941 agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc 3001 gtatttttat gaataatatt ctccgttcaa tttactgatt gtcctcgaca ggccttaagg 3061 gccagatctt gggcccggta cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat 3121 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata 3181 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc 3241 aaccacaggg ttcccctcgg gagtgcttgg cattccgtgc gataatgact tctgttcaac 3301 cacccaaacg tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc cttggctcgt 3361 ctgggtcggc tagaaggtcg agtgggctgc tgtggcttga tccctcaacg cggtcgcgga 3421 cgtagcgcag cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct 3481 gcttgtcgct ctttcggccg acgtcctggc cagtcatcac gcgccaaagt tccgtcacag 3541 gatgatctgg cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc gggaactcca 3601 cgaaaatatc cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc 3661 cgccgacgcc gttgatgtgg acgccgaaaa ggatctaggt gaagatcctt tttgataatc 3721 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 3781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 3841 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 3901 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 3961 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 4021 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 4081 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 4141 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 4201 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 4261 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 4321 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 4381 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 4441 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgattaccg cctttgagtg 4501 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 4561 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat 4621 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg 4681 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg 4741 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg 4801 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggggtacgtc 4861 gaggtcgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc 4921 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc 4981 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta 5041 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg 5101 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg 5161 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag 5221 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc 5281 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct 5341 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac 5401 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg 5461 gcgcgaggcc gccaaggcgc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc 5521 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc 5581 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt 5641 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga 5701 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac 5761 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg 5821 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt 5881 ttgtctgatg ccaagctcgc ggcctggccg gcgagcttgg ccgctgaaga aaccgagcgc 5941 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg 6001 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg 6061 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc 6121 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg 6181 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga 6241 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc 6301 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc 6361 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca 6421 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca 6481 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt 6541 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg 6601 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat 6661 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag 6721 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac 6781 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat 6841 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct 6901 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg 6961 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa 7021 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa 7081 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg 7141 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcg gcgcaggccg cccagcggca 7201 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg 7261 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg 7321 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg 7381 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt 7441 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc 7501 gagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa 7561 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga 7621 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac 7681 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg 7741 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag 7801 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat 7861 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc 7921 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag 7981 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg 8041 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga 8101 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc 8161 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa 8221 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat 8281 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat 8341 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact 8401 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga 8461 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg 8521 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc 8581 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccgg 8641 tgggtatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag 8701 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat 8761 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg 8821 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt 8881 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc 8941 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt 9001 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc 9061 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac 9121 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga 9181 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat 9241 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga 9301 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact 9361 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat 9421 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg 9481 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa 9541 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt 9601 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt 9661 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc 9721 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga 9781 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac 9841 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa 9901 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt 9961 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta 10021 tcgcaatagt tggcgaagta atcgcaacat agcttgcttg gtcgttccgc gtgaacgtcg 10081 gctcgattgt acctgcgttc aaatactttg cgatcgtgtt gcgcgcctgc ccggtgcgtc 10141 ggctgatctc acggatcgac tgcttctctc gcaacgccat ccgacggatg atgtttaaaa 10201 gtcccatgtg gatcactccg ttgccccgtc gctcaccgtg ttggggggaa ggtgcacatg 10261 gctcagttct caatggaaat tatctgccta accggctcag ttctgcgtag aaaccaacat 10321 gcaagctcca ccgggtgcaa agcggcagcg gc //