LOCUS B416p6oUH-LH 9585 bp DNA circular SYN 21-JAN-2016 DEFINITION Ligation of UbiH-BsmB-Xho* into B413p6o-LH* ACCESSION B416p6oUH-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9585) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B413p6o-LH* Fragment ends: XhoI and XbaI Fragment size: 9235 Insert molecule: UbiH-BsmB-Xho* Fragment ends: BsmBI and XhoI Fragment size: 350 Primer for gene synthesis UbiHBsmB ccCGTCTCtctagaCCGCGACAAAACTTAAG UbiHXho CCCGTCctcgagcAGACGAAGCGAAACAG Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC LB CTCAGTTCTGCGTAGAAACCA fuer Maxi FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 316..651 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_signal 538..541 /label=tata /SECDrawAs="Label" CDS 569..651 /gene="mRNA" /SECDrawAs="Gene" /SECStyleId=1 CDS 671..1240 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 1241..1429 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 1430..1885 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1896..2186 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2362..2385 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2920..3617 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3654..3913 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4166..7871 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8055..9065 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacc gcgacaaaac ttaagaaagt caccaaaaag aaagtcaaag acggcaacgg 361 caacccagaa gctacgttct ctttctagat tctatgccct acctaccgcg tggtgcacaa 421 caacttgacc cccatcattg actctccacc gtacacgtgt cacgatacca tttgttggtc 481 tcctaccact tacgtaacat taatattcca tccgtgaccg cctcttcttc atcatcctat 541 aaattcacct tcttatctct tccgctttca ttcgttcatc atcatcacaa ttaacaacaa 601 tccaaagcaa tcttctctca tattcttatc tcttcctgtt tcgcttcgtc tgctcgagtc 661 ctccataact atgaagaaac cggagctgac cgccacttcc gtcgaaaagt tcctcatcga 721 gaagtttgat agcgtgtctg accttatgca gttgtcggag ggcgaagagt cccgcgcgtt 781 cagctttgat gttggcgggc gcgggtacgt cctccgcgtg aactcttgcg ctgacggctt 841 ctataaagat cggtacgttt acaggcattt cgcctcggcg gccctgccta ttcccgaagt 901 cctcgacatc ggcgagtttt ccgagagcct tacgtattgt atttcccgcc gggctcaagg 961 ggtgaccctc caggatctgc cggaaactga gttgcctgcg gttctccagc ccgtcgccga 1021 ggctatggac gccatcgccg cggcggatct ttctcaaacg tcgggcttcg ggccgttcgg 1081 ccctcagggg attggccagt acaccacttg gagggacttt atctgcgcta tcgccgatcc 1141 gcacgtgtat cattggcaaa ccgttatgga cgatacggtc tccgcgagcg tggcccaggc 1201 tctggacgaa ctcatgttgt gggccgagga ttgccccgag gtaagtttct gcttctacct 1261 ttgatatata tataataatt atcattaatt agtagtaata taatatttca aatatttttt 1321 tcaaaataaa agaatgtagt atatagcaat tgcttttctg tagtttataa gtgtgtatat 1381 tttaatttat aacttttcta atatatgacc aaaatttgtt gatgtgcagg tccgccacct 1441 ggttcatgcg gacttcggct ctaataacgt gctcaccgat aatgggcgga ttactgccgt 1501 catcgactgg tccgaagcta tgtttggcga ttcgcagtac gaagtggcga acattttctt 1561 ctggaggcct tggcttgcct gcatggaaca gcaaacgcgc tactttgagc ggaggcaccc 1621 ggagctggcg gggtctcccc gcctcagggc ctatatgttg cggatcggcc tcgaccagct 1681 ttaccagtcc ctggttgatg ggaatttcga cgatgctgcc tgggcgcaag gccgctgtga 1741 cgctattgtc aggagcggcg cggggaccgt gggccgcact cagatcgccc ggcggtcggc 1801 tgccgtttgg acggatgggt gcgtcgaagt gctcgcggac agcggcaaca ggcggccttc 1861 cacccgcccg cgggccaagg agtgaacgcg ttcgagtatt atggcattgg gaaaactgtt 1921 tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc 1981 gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat 2041 tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata 2101 agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc 2161 gaagttaata tgaggagtaa aacactgaag cctgcaggca tgcaagctga tccactagag 2221 gccatggcgg ccgcactagt ggatcccccg ggatgcatga attcaagctt atcgatgtcg 2281 acaggcctta agggccagat cttgggcccg gtacccgatc agattgtcgt ttcccgcctt 2341 cggtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc 2401 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt 2461 gtatgtgcat gccaaccaca gggttcccct cgggagtgct tggcattccg tgcgataatg 2521 acttctgttc aaccacccaa acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga 2581 tcccttggct cgtctgggtc ggctagaagg tcgagtgggc tgctgtggct tgatccctca 2641 acgcggtcgc ggacgtagcg cagcgccgaa aaatcctcga tcgcaaatcc gacgctgtcg 2701 aaaagcgtga tctgcttgtc gctctttcgg ccgacgtcct ggccagtcat cacgcgccaa 2761 agttccgtca caggatgatc tggcgcgagt tgctggatct cgccttcaat ccgggtctgt 2821 ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtgcat ctcggtcttg 2881 cctgggcagt cgccgccgac gccgttgatg tggacgccga aaaggatcta ggtgaagatc 2941 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3001 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3061 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 3121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 3181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 3241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 3301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 3361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 3421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 3481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 3541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 3601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 3661 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgatta 3721 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 3781 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 3841 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 3901 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac 3961 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 4021 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 4081 gcaggggtac gtcgaggtcg atccaacccc tccgctgcta tagtgcagtc ggcttctgac 4141 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg 4201 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga 4261 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc 4321 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc 4381 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc 4441 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc 4501 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg 4561 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc 4621 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc 4681 gcacccggag cgggcgcgag gccgccaagg cgcgaggcgt gaagtttggc ccccgcccta 4741 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg 4801 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc 4861 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat 4921 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa 4981 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat 5041 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga 5101 aatcctggcc ggtttgtctg atgccaagct cgcggcctgg ccggcgagct tggccgctga 5161 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca 5221 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg 5281 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat 5341 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag 5401 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc 5461 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc 5521 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg 5581 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg 5641 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg 5701 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg 5761 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc 5821 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc 5881 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca 5941 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca 6001 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca 6061 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat 6121 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg 6181 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc 6241 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc 6301 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc 6361 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gcggcgcagg 6421 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg 6481 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga 6541 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca 6601 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac 6661 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc 6721 cggccggcat ggcgagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa 6781 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc 6841 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg 6901 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga 6961 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca 7021 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga 7081 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact 7141 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca 7201 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt 7261 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg 7321 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga 7381 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc 7441 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc 7501 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga 7561 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa 7621 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc 7681 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc 7741 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc 7801 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac 7861 atcaaggcac cggtgggtat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg 7921 ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac 7981 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt 8041 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg 8101 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta 8161 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga 8221 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg 8281 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc 8341 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga 8401 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc 8461 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc 8521 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag 8581 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg 8641 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg 8701 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat 8761 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg 8821 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg 8881 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg 8941 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg 9001 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca 9061 aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg 9121 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat 9181 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg 9241 ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa catagcttgc ttggtcgttc 9301 cgcgtgaacg tcggctcgat tgtacctgcg ttcaaatact ttgcgatcgt gttgcgcgcc 9361 tgcccggtgc gtcggctgat ctcacggatc gactgcttct ctcgcaacgc catccgacgg 9421 atgatgttta aaagtcccat gtggatcact ccgttgcccc gtcgctcacc gtgttggggg 9481 gaaggtgcac atggctcagt tctcaatgga aattatctgc ctaaccggct cagttctgcg 9541 tagaaaccaa catgcaagct ccaccgggtg caaagcggca gcggc //