LOCUS B417p7oUH-LH 9111 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of UbiH-BsmB-Xho* into B414p7o-LH* ACCESSION B417p7oUH-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9111) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B414p7o-LH* Fragment ends: XhoI and XbaI Fragment size: 8761 Insert molecule: UbiH-BsmB-Xho* Fragment ends: BsmBI and XhoI Fragment size: 350 Primer for gene synthesis UbiHBsmB ccCGTCTCtctagaCCGCGACAAAACTTAAG UbiHXho CCCGTCctcgagcAGACGAAGCGAAACAG Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC LB CTCAGTTCTGCGTAGAAACCA Maxi FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 316..651 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_signal 433..435 /label=cat /SECDrawAs="Label" misc_signal 436..438 /label=cat /SECDrawAs="Label" misc_signal 469..471 /label=cat /SECDrawAs="Label" misc_signal 498..500 /label=cat /SECDrawAs="Label" misc_signal 509..511 /label=cat /SECDrawAs="Label" misc_signal 530..532 /label=cat /SECDrawAs="Label" misc_signal 533..535 /label=cat /SECDrawAs="Label" misc_signal 538..541 /label=tata /SECDrawAs="Label" CDS 569..651 /gene="mRNA" /SECDrawAs="Gene" /SECStyleId=1 CDS 671..904 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 905..1093 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 1094..1409 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1422..1712 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1888..1911 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2446..3143 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3180..3439 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3692..7397 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 7581..8591 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacc gcgacaaaac ttaagaaagt caccaaaaag aaagtcaaag acggcaacgg 361 caacccagaa gctacgttct ctttctagat tctatgccct acctaccgcg tggtgcacaa 421 caacttgacc cccatcattg actctccacc gtacacgtgt cacgatacca tttgttggtc 481 tcctaccact tacgtaacat taatattcca tccgtgaccg cctcttcttc atcatcctat 541 aaattcacct tcttatctct tccgctttca ttcgttcatc atcatcacaa ttaacaacaa 601 tccaaagcaa tcttctctca tattcttatc tcttcctgtt tcgcttcgtc tgctcgagtc 661 ctccataact atgggccccg agcgcaggcc cgccgacatc cgccgtgcca ccgaggccga 721 catgccagcc gtctgcacca tcgtgaacca ctacatcgag acctccacgg tcaacttccg 781 cacagagccg caggagcctc aggagtggac cgacgacctc gtgaggctgc gcgagcgcta 841 cccctggctc gtcgctgagg tggatggcga ggtcgccggc atcgcctacg cgggcccctg 901 gaaggtaagt ttctgcttct acctttgata tatatataat aattatcatt aattagtagt 961 aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt 1021 tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt 1081 tgttgatgtg caggcacgca acgcctacga ctggacggcg gagagcaccg tctacgtgtc 1141 cccacgccac cagcgcaccg gcctgggctc cacgctctac acccacctgc tcaagagcct 1201 ggaggcccag ggcttcaagt ccgtggtcgc tgtgatcggc ctccccaacg acccgagcgt 1261 ccgtatgcac gaggccctcg gctacgcgcc ccgcgggatg ctgagggccg cgggcttcaa 1321 gcacggcaac tggcacgacg tgggcttctg gcagctcgac ttctccctgc ctgtcccacc 1381 ccgcccggtg ctccccgtca ccgaaatctg aacgcgttcg agtattatgg cattgggaaa 1441 actgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta ttcggttttc 1501 gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata tggtcctttt 1561 gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa 1621 attataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta 1681 atgaccgaag ttaatatgag gagtaaaaca ctgaagcctg caggcatgca agctgatcca 1741 ctagaggcca tggcggccgc actagtggat cccccgggat gcatgaattc aagcttatcg 1801 atgtcgacag gccttaaggg ccagatcttg ggcccggtac ccgatcagat tgtcgtttcc 1861 cgccttcggt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa 1921 aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt 1981 ccatttgtat gtgcatgcca accacagggt tcccctcggg agtgcttggc attccgtgcg 2041 ataatgactt ctgttcaacc acccaaacgt cggaaagcct gacgacggag cagcattcca 2101 aaaagatccc ttggctcgtc tgggtcggct agaaggtcga gtgggctgct gtggcttgat 2161 ccctcaacgc ggtcgcggac gtagcgcagc gccgaaaaat cctcgatcgc aaatccgacg 2221 ctgtcgaaaa gcgtgatctg cttgtcgctc tttcggccga cgtcctggcc agtcatcacg 2281 cgccaaagtt ccgtcacagg atgatctggc gcgagttgct ggatctcgcc ttcaatccgg 2341 gtctgtggcg ggaactccac gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg 2401 gtcttgcctg ggcagtcgcc gccgacgccg ttgatgtgga cgccgaaaag gatctaggtg 2461 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 2521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 2581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 2641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 2701 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 2761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 2821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 2881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 2941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 3001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 3061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 3121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 3181 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 3241 cgattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 3301 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt 3361 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt 3421 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc 3481 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 3541 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 3601 cgcgaggcag gggtacgtcg aggtcgatcc aacccctccg ctgctatagt gcagtcggct 3661 tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc 3721 gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa 3781 agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg 3841 gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg 3901 aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg 3961 accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga 4021 ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc 4081 aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg 4141 ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca 4201 tcgaccgcac ccggagcggg cgcgaggccg ccaaggcgcg aggcgtgaag tttggccccc 4261 gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc 4321 gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac 4381 ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg 4441 acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag 4501 catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc 4561 ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct 4621 gcatgaaatc ctggccggtt tgtctgatgc caagctcgcg gcctggccgg cgagcttggc 4681 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt 4741 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa 4801 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca 4861 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat 4921 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc 4981 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta 5041 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac 5101 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg 5161 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg 5221 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac 5281 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc 5341 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg 5401 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca 5461 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt 5521 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg 5581 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat 5641 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt 5701 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa 5761 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg 5821 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat 5881 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggcgg 5941 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag 6001 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga 6061 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg 6121 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg 6181 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg 6241 cagggccggc cggcatggcg agtgtgtggg attacgacct ggtactgatg gcggtttccc 6301 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt 6361 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga 6421 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta 6481 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc 6541 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg 6601 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg 6661 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg 6721 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg 6781 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg 6841 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca 6901 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa 6961 ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg 7021 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca 7081 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg 7141 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt 7201 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc 7261 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc 7321 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc 7381 gcccacatca aggcaccggt gggtatgcct gacgatgcgt ggagaccgaa accttgcgct 7441 cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac 7501 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta 7561 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca 7621 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac 7681 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt 7741 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca 7801 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg 7861 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg 7921 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa 7981 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg 8041 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt 8101 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta 8161 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac 8221 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg 8281 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg 8341 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca 8401 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc 8461 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct 8521 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag 8581 tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact 8641 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc 8701 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgaaa 8761 ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacata gcttgcttgg 8821 tcgttccgcg tgaacgtcgg ctcgattgta cctgcgttca aatactttgc gatcgtgttg 8881 cgcgcctgcc cggtgcgtcg gctgatctca cggatcgact gcttctctcg caacgccatc 8941 cgacggatga tgtttaaaag tcccatgtgg atcactccgt tgccccgtcg ctcaccgtgt 9001 tggggggaag gtgcacatgg ctcagttctc aatggaaatt atctgcctaa ccggctcagt 9061 tctgcgtaga aaccaacatg caagctccac cgggtgcaaa gcggcagcgg c //