LOCUS B433p6oU10-LH 10718 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of U10-Bsa-Xho* into B413p6o-LH* ACCESSION B433p6oU10-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10718) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B413p6o-LH* Fragment ends: XhoI and XbaI Fragment size: 9235 Insert molecule: U10-Bsa-Xho* Fragment ends: BsaI and XhoI Fragment size: 1483 Primer for gene synthesis BsaU10 ccGGTCTCtctagaTTTCCTTACATTCTGAGCC XhoU10 CCCGTCctcgaGTACCTGTTAATCAGAAAAACTC Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC hptoR GGTCAGACACGCTATCAAACT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1478 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2373 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 2374..2562 /gene="int" /product="StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="StLS1" CDS 2563..3018 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 3029..3319 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3495..3518 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4053..4750 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4787..5046 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5299..9004 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9188..10198 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 actatgaaga aaccggagct gaccgccact tccgtcgaaa agttcctcat cgagaagttt 1861 gatagcgtgt ctgaccttat gcagttgtcg gagggcgaag agtcccgcgc gttcagcttt 1921 gatgttggcg ggcgcgggta cgtcctccgc gtgaactctt gcgctgacgg cttctataaa 1981 gatcggtacg tttacaggca tttcgcctcg gcggccctgc ctattcccga agtcctcgac 2041 atcggcgagt tttccgagag ccttacgtat tgtatttccc gccgggctca aggggtgacc 2101 ctccaggatc tgccggaaac tgagttgcct gcggttctcc agcccgtcgc cgaggctatg 2161 gacgccatcg ccgcggcgga tctttctcaa acgtcgggct tcgggccgtt cggccctcag 2221 gggattggcc agtacaccac ttggagggac tttatctgcg ctatcgccga tccgcacgtg 2281 tatcattggc aaaccgttat ggacgatacg gtctccgcga gcgtggccca ggctctggac 2341 gaactcatgt tgtgggccga ggattgcccc gaggtaagtt tctgcttcta cctttgatat 2401 atatataata attatcatta attagtagta atataatatt tcaaatattt ttttcaaaat 2461 aaaagaatgt agtatatagc aattgctttt ctgtagttta taagtgtgta tattttaatt 2521 tataactttt ctaatatatg accaaaattt gttgatgtgc aggtccgcca cctggttcat 2581 gcggacttcg gctctaataa cgtgctcacc gataatgggc ggattactgc cgtcatcgac 2641 tggtccgaag ctatgtttgg cgattcgcag tacgaagtgg cgaacatttt cttctggagg 2701 ccttggcttg cctgcatgga acagcaaacg cgctactttg agcggaggca cccggagctg 2761 gcggggtctc cccgcctcag ggcctatatg ttgcggatcg gcctcgacca gctttaccag 2821 tccctggttg atgggaattt cgacgatgct gcctgggcgc aaggccgctg tgacgctatt 2881 gtcaggagcg gcgcggggac cgtgggccgc actcagatcg cccggcggtc ggctgccgtt 2941 tggacggatg ggtgcgtcga agtgctcgcg gacagcggca acaggcggcc ttccacccgc 3001 ccgcgggcca aggagtgaac gcgttcgagt attatggcat tgggaaaact gtttttcttg 3061 taccatttgt tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt 3121 gaaatggaaa tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa 3181 ttaatattat ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata 3241 tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta 3301 atatgaggag taaaacactg aagcctgcag gcatgcaagc tgatccacta gaggccatgg 3361 cggccgcact agtggatccc ccgggatgca tgaattcaag cttatcgatg tcgacaggcc 3421 ttaagggcca gatcttgggc ccggtacccg atcagattgt cgtttcccgc cttcggttta 3481 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt 3541 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg 3601 catgccaacc acagggttcc cctcgggagt gcttggcatt ccgtgcgata atgacttctg 3661 ttcaaccacc caaacgtcgg aaagcctgac gacggagcag cattccaaaa agatcccttg 3721 gctcgtctgg gtcggctaga aggtcgagtg ggctgctgtg gcttgatccc tcaacgcggt 3781 cgcggacgta gcgcagcgcc gaaaaatcct cgatcgcaaa tccgacgctg tcgaaaagcg 3841 tgatctgctt gtcgctcttt cggccgacgt cctggccagt catcacgcgc caaagttccg 3901 tcacaggatg atctggcgcg agttgctgga tctcgccttc aatccgggtc tgtggcggga 3961 actccacgaa aatatccgaa cgcagcaaga tatcgcggtg catctcggtc ttgcctgggc 4021 agtcgccgcc gacgccgttg atgtggacgc cgaaaaggat ctaggtgaag atcctttttg 4081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 4141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 4201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 4261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 4321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 4381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 4441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 4501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 4561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 4621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 4681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 4741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 4801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccga ttaccgcctt 4861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 4921 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 4981 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac 5041 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct 5101 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 5161 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggg 5221 tacgtcgagg tcgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt 5281 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc 5341 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg 5401 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta 5461 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc 5521 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct 5581 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg 5641 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc 5701 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt 5761 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg 5821 gagcgggcgc gaggccgcca aggcgcgagg cgtgaagttt ggcccccgcc ctaccctcac 5881 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga 5941 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga 6001 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga 6061 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac 6121 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg 6181 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg 6241 gccggtttgt ctgatgccaa gctcgcggcc tggccggcga gcttggccgc tgaagaaacc 6301 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc 6361 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta 6421 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc 6481 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg 6541 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc 6601 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag 6661 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc 6721 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc 6781 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca 6841 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc 6901 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg 6961 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa 7021 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac 7081 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc 7141 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct 7201 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc 7261 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag 7321 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg 7381 gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca 7441 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca 7501 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggcggcgc aggccgccca 7561 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg 7621 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc 7681 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga 7741 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg 7801 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg 7861 catggcgagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc 7921 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt 7981 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt 8041 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa 8101 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt 8161 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg 8221 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat 8281 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga 8341 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa 8401 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa 8461 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg 8521 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg 8581 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc 8641 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc 8701 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt 8761 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac 8821 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc 8881 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa 8941 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg 9001 caccggtggg tatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca 9061 ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa 9121 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa 9181 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg 9241 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg 9301 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa 9361 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt 9421 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga 9481 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca 9541 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc 9601 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc 9661 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg 9721 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc 9781 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt 9841 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc 9901 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa 9961 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa 10021 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact 10081 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca 10141 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg 10201 tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg 10261 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt 10321 aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc tggctttttc 10381 ttgttatcgc aatagttggc gaagtaatcg caacatagct tgcttggtcg ttccgcgtga 10441 acgtcggctc gattgtacct gcgttcaaat actttgcgat cgtgttgcgc gcctgcccgg 10501 tgcgtcggct gatctcacgg atcgactgct tctctcgcaa cgccatccga cggatgatgt 10561 ttaaaagtcc catgtggatc actccgttgc cccgtcgctc accgtgttgg ggggaaggtg 10621 cacatggctc agttctcaat ggaaattatc tgcctaaccg gctcagttct gcgtagaaac 10681 caacatgcaa gctccaccgg gtgcaaagcg gcagcggc //