LOCUS B466p6oU10-35s-o 11346 bp DNA circular SYN 08-FEB-2016 DEFINITION Ligation of p35-OCS-BM* into B433p6oU10-LH* ACCESSION B466p6oU10-35s-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11346) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B433p6oU10-LH* Fragment ends: SfiI Fragment size: 10654 Insert molecule: p35-OCS-BM* Fragment ends: SfiI Fragment size: 692 Sequencing primers SphR GGGTGGTTGAACAGAAGTCAT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1476 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2373 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 2374..2562 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 2563..3018 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 3029..3319 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3374..3796 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" misc_feature 3843..4038 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 4123..4146 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4681..5378 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5415..5674 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5927..9632 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9816..10826 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 actatgaaga aaccggagct gaccgccact tccgtcgaaa agttcctcat cgagaagttt 1861 gatagcgtgt ctgaccttat gcagttgtcg gagggcgaag agtcccgcgc gttcagcttt 1921 gatgttggcg ggcgcgggta cgtcctccgc gtgaactctt gcgctgacgg cttctataaa 1981 gatcggtacg tttacaggca tttcgcctcg gcggccctgc ctattcccga agtcctcgac 2041 atcggcgagt tttccgagag ccttacgtat tgtatttccc gccgggctca aggggtgacc 2101 ctccaggatc tgccggaaac tgagttgcct gcggttctcc agcccgtcgc cgaggctatg 2161 gacgccatcg ccgcggcgga tctttctcaa acgtcgggct tcgggccgtt cggccctcag 2221 gggattggcc agtacaccac ttggagggac tttatctgcg ctatcgccga tccgcacgtg 2281 tatcattggc aaaccgttat ggacgatacg gtctccgcga gcgtggccca ggctctggac 2341 gaactcatgt tgtgggccga ggattgcccc gaggtaagtt tctgcttcta cctttgatat 2401 atatataata attatcatta attagtagta atataatatt tcaaatattt ttttcaaaat 2461 aaaagaatgt agtatatagc aattgctttt ctgtagttta taagtgtgta tattttaatt 2521 tataactttt ctaatatatg accaaaattt gttgatgtgc aggtccgcca cctggttcat 2581 gcggacttcg gctctaataa cgtgctcacc gataatgggc ggattactgc cgtcatcgac 2641 tggtccgaag ctatgtttgg cgattcgcag tacgaagtgg cgaacatttt cttctggagg 2701 ccttggcttg cctgcatgga acagcaaacg cgctactttg agcggaggca cccggagctg 2761 gcggggtctc cccgcctcag ggcctatatg ttgcggatcg gcctcgacca gctttaccag 2821 tccctggttg atgggaattt cgacgatgct gcctgggcgc aaggccgctg tgacgctatt 2881 gtcaggagcg gcgcggggac cgtgggccgc actcagatcg cccggcggtc ggctgccgtt 2941 tggacggatg ggtgcgtcga agtgctcgcg gacagcggca acaggcggcc ttccacccgc 3001 ccgcgggcca aggagtgaac gcgttcgagt attatggcat tgggaaaact gtttttcttg 3061 taccatttgt tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt 3121 gaaatggaaa tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa 3181 ttaatattat ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata 3241 tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta 3301 atatgaggag taaaacactg aagcctgcag gcatgcaagc tgatccacta gaggccatgg 3361 cggccgcact agatcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa 3421 gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga 3481 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag 3541 gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc 3601 tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa 3661 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 3721 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 3781 catttggaga ggacactagt tggatccccc gggctgcagg aattcaagct tacgcgtgtc 3841 gaccctgctt taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc 3901 tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg 3961 gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt 4021 tcaatttact gattgtcctc gacaggcctt aagggccaga tcttgggccc ggtacccgat 4081 cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat atattggcgg 4141 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa 4201 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggagtgc 4261 ttggcattcc gtgcgataat gacttctgtt caaccaccca aacgtcggaa agcctgacga 4321 cggagcagca ttccaaaaag atcccttggc tcgtctgggt cggctagaag gtcgagtggg 4381 ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc gcagcgccga aaaatcctcg 4441 atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg gccgacgtcc 4501 tggccagtca tcacgcgcca aagttccgtc acaggatgat ctggcgcgag ttgctggatc 4561 tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa tatccgaacg cagcaagata 4621 tcgcggtgca tctcggtctt gcctgggcag tcgccgccga cgccgttgat gtggacgccg 4681 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 4741 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 4801 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 4861 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 4921 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 4981 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 5041 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 5101 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 5161 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 5221 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 5281 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 5341 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 5401 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 5461 gattctgtgg ataaccgatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 5521 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc 5581 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct 5641 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc 5701 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 5761 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 5821 gtcatcaccg aaacgcgcga ggcaggggta cgtcgaggtc gatccaaccc ctccgctgct 5881 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca 5941 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt 6001 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca 6061 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga 6121 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca 6181 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg 6241 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca 6301 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg 6361 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg 6421 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcgcgaggcg 6481 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga 6541 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga 6601 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg 6661 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac 6721 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac 6781 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt 6841 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tcgcggcctg 6901 gccggcgagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt 6961 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca 7021 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc 7081 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg 7141 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa 7201 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc 7261 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg 7321 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc 7381 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa 7441 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag 7501 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac 7561 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc 7621 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta 7681 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca 7741 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc 7801 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca 7861 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa 7921 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc 7981 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc 8041 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga 8101 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg 8161 agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg 8221 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag 8281 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc 8341 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc 8401 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc 8461 acgtagaggt ttccgcaggg ccggccggca tggcgagtgt gtgggattac gacctggtac 8521 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca 8581 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg 8641 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg 8701 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg 8761 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg 8821 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc 8881 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc 8941 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac 9001 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt 9061 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag 9121 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc 9181 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg 9241 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga 9301 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa 9361 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg 9421 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc 9481 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct 9541 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc 9601 cactcgaccg ccggcgccca catcaaggca ccggtgggta tgcctgacga tgcgtggaga 9661 ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca 9721 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag 9781 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa 9841 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact 9901 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg 9961 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa 10021 caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg 10081 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct 10141 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg 10201 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt 10261 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca 10321 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg 10381 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga 10441 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc 10501 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc 10561 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca 10621 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc 10681 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag 10741 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg 10801 agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg 10861 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta 10921 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg 10981 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca 11041 acatagcttg cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc gttcaaatac 11101 tttgcgatcg tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat cgactgcttc 11161 tctcgcaacg ccatccgacg gatgatgttt aaaagtccca tgtggatcac tccgttgccc 11221 cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg aaattatctg 11281 cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt gcaaagcggc 11341 agcggc //