LOCUS B467p7oU10-35s-o 10872 bp DNA circular SYN 08-FEB-2016 DEFINITION Ligation of p35-OCS-BM* into B434p7oU10-LH* ACCESSION B467p7oU10-35s-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10872) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B434p7oU10-LH* Fragment ends: SfiI Fragment size: 10180 Insert molecule: p35-OCS-BM* Fragment ends: SfiI Fragment size: 692 Sequencing primers SphR GGGTGGTTGAACAGAAGTCAT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1476 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2037 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2038..2226 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 2227..2542 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2555..2845 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2900..3322 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" misc_feature 3369..3564 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3649..3672 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4207..4904 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4941..5200 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5453..9158 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9342..10352 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 actatgggcc ccgagcgcag gcccgccgac atccgccgtg ccaccgaggc cgacatgcca 1861 gccgtctgca ccatcgtgaa ccactacatc gagacctcca cggtcaactt ccgcacagag 1921 ccgcaggagc ctcaggagtg gaccgacgac ctcgtgaggc tgcgcgagcg ctacccctgg 1981 ctcgtcgctg aggtggatgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggta 2041 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa 2101 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag 2161 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat 2221 gtgcaggcac gcaacgccta cgactggacg gcggagagca ccgtctacgt gtccccacgc 2281 caccagcgca ccggcctggg ctccacgctc tacacccacc tgctcaagag cctggaggcc 2341 cagggcttca agtccgtggt cgctgtgatc ggcctcccca acgacccgag cgtccgtatg 2401 cacgaggccc tcggctacgc gccccgcggg atgctgaggg ccgcgggctt caagcacggc 2461 aactggcacg acgtgggctt ctggcagctc gacttctccc tgcctgtccc accccgcccg 2521 gtgctccccg tcaccgaaat ctgaacgcgt tcgagtatta tggcattggg aaaactgttt 2581 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg 2641 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt 2701 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa 2761 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg 2821 aagttaatat gaggagtaaa acactgaagc ctgcaggcat gcaagctgat ccactagagg 2881 ccatggcggc cgcactagat caacatggtg gagcacgaca ctctcgtcta ctccaagaat 2941 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata 3001 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 3061 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa 3121 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa 3181 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac 3241 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 3301 tcatttcatt tggagaggac actagttgga tcccccgggc tgcaggaatt caagcttacg 3361 cgtgtcgacc ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt 3421 caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg 3481 gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt 3541 ctccgttcaa tttactgatt gtcctcgaca ggccttaagg gccagatctt gggcccggta 3601 cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat cagtgtttga caggatatat 3661 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg 3721 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg 3781 gagtgcttgg cattccgtgc gataatgact tctgttcaac cacccaaacg tcggaaagcc 3841 tgacgacgga gcagcattcc aaaaagatcc cttggctcgt ctgggtcggc tagaaggtcg 3901 agtgggctgc tgtggcttga tccctcaacg cggtcgcgga cgtagcgcag cgccgaaaaa 3961 tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct gcttgtcgct ctttcggccg 4021 acgtcctggc cagtcatcac gcgccaaagt tccgtcacag gatgatctgg cgcgagttgc 4081 tggatctcgc cttcaatccg ggtctgtggc gggaactcca cgaaaatatc cgaacgcagc 4141 aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc cgccgacgcc gttgatgtgg 4201 acgccgaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 4261 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 4321 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 4381 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 4441 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 4501 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 4561 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 4621 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 4681 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 4741 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 4801 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 4861 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 4921 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 4981 tcccctgatt ctgtggataa ccgattaccg cctttgagtg agctgatacc gctcgccgca 5041 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt 5101 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 5161 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt 5221 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 5281 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 5341 ttcaccgtca tcaccgaaac gcgcgaggca ggggtacgtc gaggtcgatc caacccctcc 5401 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt 5461 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg 5521 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca 5581 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg 5641 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga 5701 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc 5761 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac 5821 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt 5881 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg 5941 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggcgc 6001 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg 6061 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc 6121 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc 6181 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga 6241 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt 6301 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc 6361 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctcgc 6421 ggcctggccg gcgagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg 6481 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa 6541 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg 6601 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat 6661 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa 6721 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc 6781 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg 6841 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata 6901 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg 6961 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt 7021 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg 7081 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac 7141 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat 7201 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac 7261 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca 7321 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca 7381 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat 7441 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa 7501 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta 7561 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg 7621 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc 7681 tggtggagaa gttgaaggcg gcgcaggccg cccagcggca acgcatcgag gcagaagcac 7741 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc 7801 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt 7861 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg 7921 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag 7981 acgggcacgt agaggtttcc gcagggccgg ccggcatggc gagtgtgtgg gattacgacc 8041 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg 8101 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc 8161 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca 8221 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg 8281 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt 8341 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg 8401 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct 8461 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct 8521 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga 8581 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga 8641 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta 8701 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc 8761 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca 8821 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag 8881 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc 8941 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta 9001 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg 9061 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc 9121 cgtcgccact cgaccgccgg cgcccacatc aaggcaccgg tgggtatgcc tgacgatgcg 9181 tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc 9241 tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga 9301 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt 9361 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt 9421 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg 9481 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc 9541 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat 9601 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 9661 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 9721 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 9781 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 9841 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 9901 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 9961 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 10021 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 10081 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 10141 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 10201 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac 10261 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga 10321 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc 10381 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc 10441 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa 10501 attgggagat atatcatgaa aggctggctt tttcttgtta tcgcaatagt tggcgaagta 10561 atcgcaacat agcttgcttg gtcgttccgc gtgaacgtcg gctcgattgt acctgcgttc 10621 aaatactttg cgatcgtgtt gcgcgcctgc ccggtgcgtc ggctgatctc acggatcgac 10681 tgcttctctc gcaacgccat ccgacggatg atgtttaaaa gtcccatgtg gatcactccg 10741 ttgccccgtc gctcaccgtg ttggggggaa ggtgcacatg gctcagttct caatggaaat 10801 tatctgccta accggctcag ttctgcgtag aaaccaacat gcaagctcca ccgggtgcaa 10861 agcggcagcg gc //