LOCUS B468p9oU10-35s-o 11124 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of p35-OCS-BM* into B435p9oU10-LH* ACCESSION B468p9oU10-35s-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11124) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B435p9oU10-LH* Fragment ends: SfiI Fragment size: 10432 Insert molecule: p35-OCS-BM* Fragment ends: SfiI Fragment size: 692 Sequencing primers SphR GGGTGGTTGAACAGAAGTCAT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1476 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2102 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 2099..2102 /label=splice /SECDrawAs="Label" CDS 2103..2289 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 2288..2291 /label=splice /SECDrawAs="Label" CDS 2290..2793 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2807..3097 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3152..3574 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" misc_feature 3621..3816 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3901..3924 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4459..5156 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5193..5452 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5705..9410 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9594..10604 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 actatgggag catggattga gcaggatggc ctccatgctg gtagccccgc tgcttgggtg 1861 gagcgcctct ttggctacga ctgggcccaa cagaccattg ggtgcagtga tgccgctgtc 1921 ttccgtttga gcgcccaggg caggccagtt ctcttcgtga agactgacct ctccggtgcc 1981 ctcaacgagt tgcaggacga agctgcccgc ctctcatggc ttgccaccac cggagtccct 2041 tgcgctgccg tgttggacgt tgtcacagag gccggaagag actggctgct cttgggcgag 2101 gtaagtttct gcttctacct ttgatatata tataataatt atcattaatt agtagtaata 2161 taatatttca aatatttttt tcaaaataaa agaatgtagt atatagcaat tgcttttctg 2221 tagtttataa gtgtgtatat tttaatttat aacttttcta atatatgacc aaaatttgtt 2281 gatgtgcagg tgccaggcca agacctcctg agctcccacc tggccccagc cgagaaggtc 2341 tccatcatgg cagacgccat gcgtcgcctc cacaccctgg acccagccac ctgccccttc 2401 gaccaccagg ccaagcacag gatcgaacgt gcacgcacta ggatggaggc cggccttgtg 2461 gaccaggatg accttgacga ggagcaccag ggccttgctc ccgccgagct gttcgcccgc 2521 ctcaaggcta gaatgcctga cggagaggac ttggtggtca cccacgggga tgcctgcctc 2581 cccaacatca tggttgagaa cggacgcttc tctggcttca tcgactgcgg tcgtctgggc 2641 gtggccgacc gctaccaaga catcgccctg gccaccaggg acatcgctga ggaacttgga 2701 ggggagtggg ccgatcgttt cttggtcctc tacggcattg ctgcccccga ctcccagagg 2761 atcgccttct accgcctgct tgacgagttc ttctgaacgc gttcgagtat tatggcattg 2821 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg 2881 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc 2941 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat 3001 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc 3061 ctctaatgac cgaagttaat atgaggagta aaacactgaa gcctgcaggc atgcaagctg 3121 atccactaga ggccatggcg gccgcactag atcaacatgg tggagcacga cactctcgtc 3181 tactccaaga atatcaaaga tacagtctca gaagaccaga gggctattga gacttttcaa 3241 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc 3301 gaaaggacag tagaaaagga agatggcttc tacaaatgcc atcattgcga taaaggaaag 3361 gctatcgttc aagatgcctc taccgacagt ggtcccaaag atggaccccc acccacgagg 3421 aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat 3481 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct 3541 atataaggaa gttcatttca tttggagagg acactagttg gatcccccgg gctgcaggaa 3601 ttcaagctta cgcgtgtcga ccctgcttta atgagatatg cgagacgcct atgatcgcat 3661 gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga 3721 tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt 3781 atgaataata ttctccgttc aatttactga ttgtcctcga caggccttaa gggccagatc 3841 ttgggcccgg tacccgatca gattgtcgtt tcccgccttc ggtttaaact atcagtgttt 3901 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata 3961 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag 4021 ggttcccctc gggagtgctt ggcattccgt gcgataatga cttctgttca accacccaaa 4081 cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat cccttggctc gtctgggtcg 4141 gctagaaggt cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc 4201 agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg 4261 ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac aggatgatct 4321 ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg gcgggaactc cacgaaaata 4381 tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc gccgccgacg 4441 ccgttgatgt ggacgccgaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 4501 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 4561 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 4621 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 4681 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 4741 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 4801 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 4861 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 4921 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 4981 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 5041 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 5101 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 5161 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 5221 tttcctgcgt tatcccctga ttctgtggat aaccgattac cgcctttgag tgagctgata 5281 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 5341 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca 5401 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct 5461 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg 5521 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 5581 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg caggggtacg tcgaggtcga 5641 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg 5701 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct 5761 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga 5821 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca 5881 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc 5941 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg 6001 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca 6061 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc 6121 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg 6181 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg 6241 ccgccaaggc gcgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg 6301 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc 6361 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca 6421 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg 6481 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga 6541 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt 6601 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga 6661 tgccaagctc gcggcctggc cggcgagctt ggccgctgaa gaaaccgagc gccgccgtct 6721 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat 6781 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac 6841 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc 6901 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg 6961 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc 7021 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg 7081 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc 7141 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc 7201 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc 7261 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc 7321 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa 7381 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc 7441 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc 7501 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca 7561 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg 7621 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt 7681 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa 7741 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt 7801 tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag 7861 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc 7921 tgggtgatga cctggtggag aagttgaagg cggcgcaggc cgcccagcgg caacgcatcg 7981 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat 8041 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc 8101 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca 8161 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct 8221 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gcgagtgtgt 8281 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc 8341 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca 8401 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc 8461 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg 8521 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg 8581 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag 8641 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg 8701 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt 8761 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg 8821 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc 8881 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg 8941 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa 9001 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc 9061 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga 9121 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa 9181 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc 9241 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta 9301 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg 9361 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ggtgggtatg 9421 cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct 9481 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac 9541 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct 9601 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt 9661 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc 9721 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc 9781 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat 9841 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg 9901 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt 9961 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc 10021 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca 10081 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg 10141 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg 10201 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg 10261 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct 10321 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt 10381 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg 10441 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg 10501 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg 10561 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt 10621 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa 10681 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata 10741 agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt tatcgcaata 10801 gttggcgaag taatcgcaac atagcttgct tggtcgttcc gcgtgaacgt cggctcgatt 10861 gtacctgcgt tcaaatactt tgcgatcgtg ttgcgcgcct gcccggtgcg tcggctgatc 10921 tcacggatcg actgcttctc tcgcaacgcc atccgacgga tgatgtttaa aagtcccatg 10981 tggatcactc cgttgccccg tcgctcaccg tgttgggggg aaggtgcaca tggctcagtt 11041 ctcaatggaa attatctgcc taaccggctc agttctgcgt agaaaccaac atgcaagctc 11101 caccgggtgc aaagcggcag cggc //