LOCUS B469pRedU10-35s- 10823 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of Red-E9-Xho-Pst* into B468p9oU10-35s-ocs-LH* ACCESSION B469pRedU10-35s- KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10823) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B468p9oU10-35s-ocs-LH* Fragment ends: SbfI and XhoI Fragment size: 9803 Insert molecule: Red-E9-Xho-Pst* Fragment ends: XhoI and PstI Fragment size: 1020 Primer for gene synthesis RedXho cccgtcCTCGAGtcctccatAACAATGCAGATCTTTGCC RedR gccataatactccggaCTACAGGAACAGGTG E9For cctgtagtccggaGTATTATGGCATTGGGAAAAC T35Sbf CTCTAGTGGATCAGCTTGCATGCCT Sequencing primers Ubq10-F CTAGATCTGGTGTTAGTTTCTAGT GC-REICH FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1476 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2499 /gene="dsRed" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 2160 /label=change to T /SECDrawAs="Label" misc_feature 2516..2796 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2851..3273 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" misc_feature 3320..3515 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3600..3623 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4158..4855 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4892..5151 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5404..9109 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9293..10303 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 acaatgcaga tctttgccat ggcgcgctcc tccaagaacg tcatcaagga gttcatgcgc 1861 ttcaaggtgc gcatggaggg caccgtgaac ggccacgagt tcgagatcga gggcgagggc 1921 gagggccgcc cctacgaggg ccacaacacc gtgaagctga aggtgaccaa gggcggcccc 1981 ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg 2041 aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg 2101 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctt 2161 caggacggct gcttcatcta caaggtgaag ttcatcggcg tgaacttccc ctccgacggc 2221 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc 2281 gacggcgtgc tgaagggcga gatccacaag gccctgaagc tgaaggacgg cggccactac 2341 ctggtggagt tcaagtccat ctacatggcc aagaagcccg tgcagctgcc cggctactac 2401 tacgtggact ccaagctgga catcacctcc cacaacgagg actacaccat cgtggagcag 2461 tacgagcgca ccgagggccg ccaccacctg ttcctgtagt ccggagtatt atggcattgg 2521 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt 2581 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc 2641 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt 2701 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc 2761 tctaatgacc gaagttaata tgaggagtaa aacactgaag cctgcaggca tgcaagctga 2821 tccactagag gccatggcgg ccgcactaga tcaacatggt ggagcacgac actctcgtct 2881 actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag acttttcaac 2941 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg 3001 aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat aaaggaaagg 3061 ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga 3121 acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata 3181 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta 3241 tataaggaag ttcatttcat ttggagagga cactagttgg atcccccggg ctgcaggaat 3301 tcaagcttac gcgtgtcgac cctgctttaa tgagatatgc gagacgccta tgatcgcatg 3361 atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat 3421 ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta 3481 tgaataatat tctccgttca atttactgat tgtcctcgac aggccttaag ggccagatct 3541 tgggcccggt acccgatcag attgtcgttt cccgccttcg gtttaaacta tcagtgtttg 3601 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat 3661 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg 3721 gttcccctcg ggagtgcttg gcattccgtg cgataatgac ttctgttcaa ccacccaaac 3781 gtcggaaagc ctgacgacgg agcagcattc caaaaagatc ccttggctcg tctgggtcgg 3841 ctagaaggtc gagtgggctg ctgtggcttg atccctcaac gcggtcgcgg acgtagcgca 3901 gcgccgaaaa atcctcgatc gcaaatccga cgctgtcgaa aagcgtgatc tgcttgtcgc 3961 tctttcggcc gacgtcctgg ccagtcatca cgcgccaaag ttccgtcaca ggatgatctg 4021 gcgcgagttg ctggatctcg ccttcaatcc gggtctgtgg cgggaactcc acgaaaatat 4081 ccgaacgcag caagatatcg cggtgcatct cggtcttgcc tgggcagtcg ccgccgacgc 4141 cgttgatgtg gacgccgaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 4201 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 4261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 4321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 4381 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 4441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 4501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 4561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 4621 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 4681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 4741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 4801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 4861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 4921 ttcctgcgtt atcccctgat tctgtggata accgattacc gcctttgagt gagctgatac 4981 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 5041 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac 5101 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta 5161 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg 5221 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 5281 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc aggggtacgt cgaggtcgat 5341 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga 5401 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg 5461 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag 5521 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac 5581 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct 5641 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga 5701 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac 5761 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct 5821 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc 5881 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc 5941 cgccaaggcg cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc 6001 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct 6061 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac 6121 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc 6181 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac 6241 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc 6301 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat 6361 gccaagctcg cggcctggcc ggcgagcttg gccgctgaag aaaccgagcg ccgccgtcta 6421 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg 6481 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc 6541 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg 6601 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg 6661 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg 6721 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg 6781 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc 6841 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca 6901 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg 6961 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca 7021 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac 7081 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca 7141 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg 7201 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat 7261 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt 7321 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta 7381 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa 7441 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt 7501 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc 7561 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct 7621 gggtgatgac ctggtggaga agttgaaggc ggcgcaggcc gcccagcggc aacgcatcga 7681 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc 7741 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca 7801 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat 7861 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta 7921 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg cgagtgtgtg 7981 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg 8041 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa 8101 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg 8161 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt 8221 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg 8281 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg 8341 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg 8401 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt 8461 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt 8521 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca 8581 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg 8641 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa 8701 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg 8761 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac 8821 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac 8881 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca 8941 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat 9001 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg 9061 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccg gtgggtatgc 9121 ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc 9181 gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg 9241 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc 9301 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt 9361 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca 9421 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca 9481 gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc 9541 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc 9601 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt 9661 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct 9721 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat 9781 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg 9841 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga 9901 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc 9961 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt 10021 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt 10081 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga 10141 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc 10201 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt 10261 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc 10321 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat 10381 tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa 10441 gccctacaca aattgggaga tatatcatga aaggctggct ttttcttgtt atcgcaatag 10501 ttggcgaagt aatcgcaaca tagcttgctt ggtcgttccg cgtgaacgtc ggctcgattg 10561 tacctgcgtt caaatacttt gcgatcgtgt tgcgcgcctg cccggtgcgt cggctgatct 10621 cacggatcga ctgcttctct cgcaacgcca tccgacggat gatgtttaaa agtcccatgt 10681 ggatcactcc gttgccccgt cgctcaccgt gttgggggga aggtgcacat ggctcagttc 10741 tcaatggaaa ttatctgcct aaccggctca gttctgcgta gaaaccaaca tgcaagctcc 10801 accgggtgca aagcggcagc ggc //