LOCUS B638p5oU10-2 11287 bp DNA circular SYN 08-FEB-2016 DEFINITION Ligation of esps-Xho-Mlu* into B435p9oU10-LH* ACCESSION B638p5oU10-2 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11287) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B435p9oU10-LH* Fragment ends: MluI and XhoI Fragment size: 9485 Insert molecule: esps-Xho-Mlu* Fragment ends: XhoI and MluI Fragment size: 1802 Primer for gene synthesis CPTDi CCCGTCctcgagTCCTCCAtaactATGGCTCAAGTCTCCC CPTBspE TCCGGATGCGGGCTCCCA BspEepsp ggctatgggagcccgcaTCCGGAAGGAAGGCGAC E9Mlu CCCAATGCCATAATACTCGAACGCG Sequencing primers Ubq10-F CTAGATCTGGTGTTAGTTTCTAGT RBCS-TR2 CACAGTAAATTACAAGCACAAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1476 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2022 /gene="cpt" /SECDrawAs="Gene" /SECStyleId=1 CDS 2023..2556 /gene="EPSPS" /codon_start=3 /translation="CLHVARCLQPSGDRQEVFRPLGDRQNPRRQEHFPPLIHVRRPGQ RRDKDHRPSGG" /SECDrawAs="Gene" /SECStyleId=1 /SECName="epsps" misc_feature 2557..2745 /gene="int" /product="Intron STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron STLS1" CDS 2746..3588 /gene="epsps" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 3598..3888 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4064..4087 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4622..5319 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5356..5615 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5868..9573 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9757..10767 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 actatggctc aagtctcccg tatctgcaac ggcgtgcaga accccagcct catttctaac 1861 ttgtcgaagt ccagccagcg caagtcccct ctgtcagtta gcttgaagac tcagcagcac 1921 ccaagagcct acccgatcag ttccagctgg ggtctcaaga agtccgtaat gacccttatc 1981 ggctctgagc tgaggcccct caaagtcatg tcgagcgtgt ccactgcttg catgttgcac 2041 ggtgcctcca gccgtccggc gaccgccagg aagtcttccg gcctctcggg gaccgtcaga 2101 atccccggag acaagagcat ttcccaccgc tcattcatgt tcggcggcct ggccagcgga 2161 gagacaagga tcaccggcct tctggagggt gaggatgtca tcaacaccgg gaaggctatg 2221 caggctatgg gagcccgcat ccggaaggaa ggcgacacgt ggattatcga cggcgtggga 2281 aacggcggtc tcctcgcccc agaggcaccc ttggacttcg gcaacgccgc caccggatgc 2341 cgtctgacta tggggcttgt tggcgtgtac gatttcgact ccaccttcat cggagacgct 2401 tccctcacca agcgccctat gggcagggtc ctaaatccgc tccgtgagat gggcgtgcaa 2461 gtcaagagcg aggacggaga ccgcctgcca gttaccttgc gaggccccaa gactccaaca 2521 cccatcacct acagggtgcc tatggcctct gcgcaggtaa gtttctgctt ctacctttga 2581 tatatatata ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa 2641 aataaaagaa tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta 2701 atttataact tttctaatat atgaccaaaa tttgttgatg tgcaggtcaa gtccgccgtg 2761 ctccttgccg ggctgaacac tccgggtatt accaccgtca tcgagcccat tatgaccaga 2821 gatcataccg agaagatgct ccagggattc ggcgctaact tgacggttga aaccgacgcc 2881 gacggcgtgc gcaccatccg tctggaggga aggggcaaac tcaccggcca agtcatcgac 2941 gtgccaggag atcccagctc gactgctttt ccacttgtag ccgccctctt ggtccctggt 3001 tccgacgtga ccatcctgaa cgttcttatg aacccgaccc gcaccgggct catcttgact 3061 ctccaggaga tgggcgctga catcgaagtc attaacccca ggctggccgg aggcgaggac 3121 gtggcagatt tgcgcgtccg tagttccacc ctgaagggtg tgacagttcc agaggaccgg 3181 gctcccagca tgatcgacga gtaccctatc cttgccgtcg ccgcggcctt cgccgagggc 3241 gctaccgtga tgaacggatt ggaggagctg cgcgtcaagg agtcagacag actctccgcc 3301 gtggccaatg ggctcaagct caacggcgtt gactgcgatg agggagaaac cagccttgtc 3361 gtgcgtggca ggccggacgg caagggactc ggtaacgctt ctggggccgc agtcgccacg 3421 cacctggacc accgcatcgc gatgtccttc ctcgtgatgg gccttgtttc ggagaaccca 3481 gtcaccgtgg acgatgccac catgattgct actagcttcc ccgagttcat ggacttgatg 3541 gccggactgg gagccaagat cgagctgtcc gacaccaagg ctgcctaacg cgttcgagta 3601 ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa tttactgtgt 3661 tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa gagttaatga 3721 atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct cttatttgtt 3781 gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt aaaaatgtgt 3841 caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga agcctgcagg 3901 catgcaagct gatccactag aggccatggc ggccgcacta gtggatcccc cgggatgcat 3961 gaattcaagc ttatcgatgt cgacaggcct taagggccag atcttgggcc cggtacccga 4021 tcagattgtc gtttcccgcc ttcggtttaa actatcagtg tttgacagga tatattggcg 4081 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa 4141 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtg 4201 cttggcattc cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg 4261 acggagcagc attccaaaaa gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg 4321 gctgctgtgg cttgatccct caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc 4381 gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc 4441 ctggccagtc atcacgcgcc aaagttccgt cacaggatga tctggcgcga gttgctggat 4501 ctcgccttca atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat 4561 atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc 4621 gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 4681 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 4741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 4801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 4861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 4921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 4981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 5041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 5101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 5161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 5221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 5281 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 5341 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 5401 tgattctgtg gataaccgat taccgccttt gagtgagctg ataccgctcg ccgcagccga 5461 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt 5521 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc 5581 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg 5641 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 5701 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 5761 cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc cctccgctgc 5821 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac 5881 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg 5941 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac 6001 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg 6061 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc 6121 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc 6181 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac 6241 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc 6301 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc 6361 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcgcgaggc 6421 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg 6481 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg 6541 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc 6601 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa 6661 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta 6721 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg 6781 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct 6841 ggccggcgag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat 6901 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac 6961 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg 7021 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct 7081 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca 7141 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg 7201 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc 7261 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc 7321 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca 7381 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga 7441 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta 7501 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc 7561 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt 7621 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc 7681 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt 7741 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc 7801 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata 7861 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg 7921 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg 7981 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg 8041 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg 8101 gagaagttga aggcggcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc 8161 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca 8221 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt 8281 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc 8341 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg 8401 cacgtagagg tttccgcagg gccggccggc atggcgagtg tgtgggatta cgacctggta 8461 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac 8521 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc 8581 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac 8641 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt 8701 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc 8761 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg 8821 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc 8881 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa 8941 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg 9001 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta 9061 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag 9121 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg 9181 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg 9241 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa 9301 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg 9361 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt 9421 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc 9481 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg 9541 ccactcgacc gccggcgccc acatcaaggc accggtgggt atgcctgacg atgcgtggag 9601 accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc 9661 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa 9721 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga 9781 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac 9841 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg 9901 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa 9961 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag 10021 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc 10081 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc 10141 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct 10201 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac 10261 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat 10321 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg 10381 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat 10441 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg 10501 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac 10561 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc 10621 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa 10681 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc 10741 gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc 10801 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact 10861 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg 10921 gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc 10981 aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata 11041 ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga tcgactgctt 11101 ctctcgcaac gccatccgac ggatgatgtt taaaagtccc atgtggatca ctccgttgcc 11161 ccgtcgctca ccgtgttggg gggaaggtgc acatggctca gttctcaatg gaaattatct 11221 gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg 11281 cagcggc //