LOCUS B434p7oU10-LH 10244 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of U10-Bsa-Xho* into B414p7o-LH* ACCESSION B434p7oU10-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10244) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B414p7o-LH* Fragment ends: XhoI and XbaI Fragment size: 8761 Insert molecule: U10-Bsa-Xho* Fragment ends: BsaI and XhoI Fragment size: 1483 Primer for gene synthesis BsaU10 ccGGTCTCtctagaTTTCCTTACATTCTGAGCC XhoU10 CCCGTCctcgaGTACCTGTTAATCAGAAAAACTC Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC baroR GGTCTCGATGTAGTGGTTCA FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1476 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2037 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2038..2226 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 2227..2542 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2555..2845 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3021..3044 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3579..4276 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4313..4572 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4825..8530 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8714..9724 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 actatgggcc ccgagcgcag gcccgccgac atccgccgtg ccaccgaggc cgacatgcca 1861 gccgtctgca ccatcgtgaa ccactacatc gagacctcca cggtcaactt ccgcacagag 1921 ccgcaggagc ctcaggagtg gaccgacgac ctcgtgaggc tgcgcgagcg ctacccctgg 1981 ctcgtcgctg aggtggatgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggta 2041 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa 2101 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag 2161 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat 2221 gtgcaggcac gcaacgccta cgactggacg gcggagagca ccgtctacgt gtccccacgc 2281 caccagcgca ccggcctggg ctccacgctc tacacccacc tgctcaagag cctggaggcc 2341 cagggcttca agtccgtggt cgctgtgatc ggcctcccca acgacccgag cgtccgtatg 2401 cacgaggccc tcggctacgc gccccgcggg atgctgaggg ccgcgggctt caagcacggc 2461 aactggcacg acgtgggctt ctggcagctc gacttctccc tgcctgtccc accccgcccg 2521 gtgctccccg tcaccgaaat ctgaacgcgt tcgagtatta tggcattggg aaaactgttt 2581 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg 2641 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt 2701 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa 2761 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg 2821 aagttaatat gaggagtaaa acactgaagc ctgcaggcat gcaagctgat ccactagagg 2881 ccatggcggc cgcactagtg gatcccccgg gatgcatgaa ttcaagctta tcgatgtcga 2941 caggccttaa gggccagatc ttgggcccgg tacccgatca gattgtcgtt tcccgccttc 3001 ggtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg 3061 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg 3121 tatgtgcatg ccaaccacag ggttcccctc gggagtgctt ggcattccgt gcgataatga 3181 cttctgttca accacccaaa cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat 3241 cccttggctc gtctgggtcg gctagaaggt cgagtgggct gctgtggctt gatccctcaa 3301 cgcggtcgcg gacgtagcgc agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga 3361 aaagcgtgat ctgcttgtcg ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa 3421 gttccgtcac aggatgatct ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg 3481 gcgggaactc cacgaaaata tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc 3541 ctgggcagtc gccgccgacg ccgttgatgt ggacgccgaa aaggatctag gtgaagatcc 3601 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 3661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 3721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 3781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 3841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 3901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 3961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 4021 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 4081 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 4141 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 4201 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 4261 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 4321 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgattac 4381 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 4441 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 4501 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 4561 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca 4621 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 4681 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg 4741 caggggtacg tcgaggtcga tccaacccct ccgctgctat agtgcagtcg gcttctgacg 4801 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc 4861 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa 4921 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct 4981 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca 5041 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc 5101 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct 5161 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc 5221 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg 5281 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg 5341 cacccggagc gggcgcgagg ccgccaaggc gcgaggcgtg aagtttggcc cccgccctac 5401 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt 5461 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg 5521 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt 5581 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa 5641 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg 5701 atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa 5761 atcctggccg gtttgtctga tgccaagctc gcggcctggc cggcgagctt ggccgctgaa 5821 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat 5881 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga 5941 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc 6001 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg 6061 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg 6121 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg 6181 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc 6241 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg 6301 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg 6361 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc 6421 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca 6481 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg 6541 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac 6601 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag 6661 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac 6721 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata 6781 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg 6841 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca 6901 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca 6961 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc 7021 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg cggcgcaggc 7081 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc 7141 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa 7201 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac 7261 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg 7321 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc 7381 ggccggcatg gcgagtgtgt gggattacga cctggtactg atggcggttt cccatctaac 7441 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc 7501 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga 7561 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa 7621 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa 7681 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat 7741 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt 7801 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa 7861 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt 7921 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga 7981 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat 8041 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct 8101 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc 8161 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa 8221 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa 8281 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca 8341 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc 8401 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc 8461 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca 8521 tcaaggcacc ggtgggtatg cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc 8581 cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc 8641 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta 8701 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg 8761 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat 8821 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag 8881 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga 8941 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca 9001 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa 9061 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg 9121 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct 9181 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc 9241 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga 9301 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt 9361 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt 9421 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga 9481 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc 9541 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt 9601 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc 9661 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa 9721 ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt 9781 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt 9841 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc 9901 tttttcttgt tatcgcaata gttggcgaag taatcgcaac atagcttgct tggtcgttcc 9961 gcgtgaacgt cggctcgatt gtacctgcgt tcaaatactt tgcgatcgtg ttgcgcgcct 10021 gcccggtgcg tcggctgatc tcacggatcg actgcttctc tcgcaacgcc atccgacgga 10081 tgatgtttaa aagtcccatg tggatcactc cgttgccccg tcgctcaccg tgttgggggg 10141 aaggtgcaca tggctcagtt ctcaatggaa attatctgcc taaccggctc agttctgcgt 10201 agaaaccaac atgcaagctc caccgggtgc aaagcggcag cggc //