LOCUS B435p9oU10-LH 10496 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of U10-Bsa-Xho* into B415p9o-LH* ACCESSION B435p9oU10-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10496) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B415p9o-LH* Fragment ends: XhoI and XbaI Fragment size: 9013 Insert molecule: U10-Bsa-Xho* Fragment ends: BsaI and XhoI Fragment size: 1483 Primer for gene synthesis BsaU10 ccGGTCTCtctagaTTTCCTTACATTCTGAGCC XhoU10 CCCGTCctcgaGTACCTGTTAATCAGAAAAACTC Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC nptoR CACTGCACCCAATGGTCTGTT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1476 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2102 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 2099..2102 /label=splice /SECDrawAs="Label" CDS 2103..2289 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 2288..2291 /label=splice /SECDrawAs="Label" CDS 2290..2793 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2807..3097 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3273..3296 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3831..4528 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4565..4824 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5077..8782 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8966..9976 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 actatgggag catggattga gcaggatggc ctccatgctg gtagccccgc tgcttgggtg 1861 gagcgcctct ttggctacga ctgggcccaa cagaccattg ggtgcagtga tgccgctgtc 1921 ttccgtttga gcgcccaggg caggccagtt ctcttcgtga agactgacct ctccggtgcc 1981 ctcaacgagt tgcaggacga agctgcccgc ctctcatggc ttgccaccac cggagtccct 2041 tgcgctgccg tgttggacgt tgtcacagag gccggaagag actggctgct cttgggcgag 2101 gtaagtttct gcttctacct ttgatatata tataataatt atcattaatt agtagtaata 2161 taatatttca aatatttttt tcaaaataaa agaatgtagt atatagcaat tgcttttctg 2221 tagtttataa gtgtgtatat tttaatttat aacttttcta atatatgacc aaaatttgtt 2281 gatgtgcagg tgccaggcca agacctcctg agctcccacc tggccccagc cgagaaggtc 2341 tccatcatgg cagacgccat gcgtcgcctc cacaccctgg acccagccac ctgccccttc 2401 gaccaccagg ccaagcacag gatcgaacgt gcacgcacta ggatggaggc cggccttgtg 2461 gaccaggatg accttgacga ggagcaccag ggccttgctc ccgccgagct gttcgcccgc 2521 ctcaaggcta gaatgcctga cggagaggac ttggtggtca cccacgggga tgcctgcctc 2581 cccaacatca tggttgagaa cggacgcttc tctggcttca tcgactgcgg tcgtctgggc 2641 gtggccgacc gctaccaaga catcgccctg gccaccaggg acatcgctga ggaacttgga 2701 ggggagtggg ccgatcgttt cttggtcctc tacggcattg ctgcccccga ctcccagagg 2761 atcgccttct accgcctgct tgacgagttc ttctgaacgc gttcgagtat tatggcattg 2821 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg 2881 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc 2941 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat 3001 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc 3061 ctctaatgac cgaagttaat atgaggagta aaacactgaa gcctgcaggc atgcaagctg 3121 atccactaga ggccatggcg gccgcactag tggatccccc gggatgcatg aattcaagct 3181 tatcgatgtc gacaggcctt aagggccaga tcttgggccc ggtacccgat cagattgtcg 3241 tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa 3301 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg 3361 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggagtgc ttggcattcc 3421 gtgcgataat gacttctgtt caaccaccca aacgtcggaa agcctgacga cggagcagca 3481 ttccaaaaag atcccttggc tcgtctgggt cggctagaag gtcgagtggg ctgctgtggc 3541 ttgatccctc aacgcggtcg cggacgtagc gcagcgccga aaaatcctcg atcgcaaatc 3601 cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg gccgacgtcc tggccagtca 3661 tcacgcgcca aagttccgtc acaggatgat ctggcgcgag ttgctggatc tcgccttcaa 3721 tccgggtctg tggcgggaac tccacgaaaa tatccgaacg cagcaagata tcgcggtgca 3781 tctcggtctt gcctgggcag tcgccgccga cgccgttgat gtggacgccg aaaaggatct 3841 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 3901 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 3961 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 4021 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 4081 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 4141 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 4201 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 4261 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 4321 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 4381 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 4441 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 4501 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 4561 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 4621 ataaccgatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 4681 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 4741 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 4801 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 4861 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 4921 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 4981 aaacgcgcga ggcaggggta cgtcgaggtc gatccaaccc ctccgctgct atagtgcagt 5041 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt 5101 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg 5161 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc 5221 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg 5281 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag 5341 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac 5401 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg 5461 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac 5521 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct 5581 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcgcgaggcg tgaagtttgg 5641 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga 5701 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg 5761 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg 5821 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga 5881 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc 5941 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg 6001 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tcgcggcctg gccggcgagc 6061 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac 6121 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag 6181 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca 6241 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt 6301 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg 6361 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact 6421 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag 6481 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc 6541 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg 6601 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg 6661 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg 6721 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc 6781 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat 6841 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc 6901 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc 6961 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct 7021 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat 7081 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg 7141 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct 7201 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa 7261 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa 7321 ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg 7381 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc 7441 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta 7501 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa 7561 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt 7621 ttccgcaggg ccggccggca tggcgagtgt gtgggattac gacctggtac tgatggcggt 7681 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg 7741 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa 7801 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca 7861 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat 7921 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct 7981 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca 8041 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg 8101 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag 8161 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct 8221 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta 8281 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg 8341 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta 8401 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc 8461 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt 8521 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata 8581 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg 8641 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc 8701 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg 8761 ccggcgccca catcaaggca ccggtgggta tgcctgacga tgcgtggaga ccgaaacctt 8821 gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg 8881 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt 8941 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga 9001 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg 9061 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt 9121 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa 9181 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt 9241 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga 9301 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga 9361 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga 9421 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg 9481 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc 9541 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag 9601 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga 9661 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg 9721 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac 9781 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca 9841 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt 9901 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa 9961 ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct 10021 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag 10081 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca 10141 tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca acatagcttg 10201 cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc gttcaaatac tttgcgatcg 10261 tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat cgactgcttc tctcgcaacg 10321 ccatccgacg gatgatgttt aaaagtccca tgtggatcac tccgttgccc cgtcgctcac 10381 cgtgttgggg ggaaggtgca catggctcag ttctcaatgg aaattatctg cctaaccggc 10441 tcagttctgc gtagaaacca acatgcaagc tccaccgggt gcaaagcggc agcggc //