LOCUS B437p7oU10-oGFPi 11364 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of B444pmGFPD-ocs* into B434p7oU10-LH* ACCESSION B437p7oU10-oGFPi KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11364) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B434p7oU10-LH* Fragment ends: SfiI Fragment size: 10180 Insert molecule: B444pmGFPD-ocs* Fragment ends: SfiI Fragment size: 1184 Sequencing primers GFP-seq CCAGTTCCACCAGGATTG SphR GGGTGGTTGAACAGAAGTCAT Maxi FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1476 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2037 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2038..2226 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 2227..2542 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2555..2845 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 CDS 2947..3325 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 3326..3514 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 3515..3852 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 3861..4056 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 4141..4164 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4699..5396 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5433..5692 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5945..9650 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9834..10844 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 actatgggcc ccgagcgcag gcccgccgac atccgccgtg ccaccgaggc cgacatgcca 1861 gccgtctgca ccatcgtgaa ccactacatc gagacctcca cggtcaactt ccgcacagag 1921 ccgcaggagc ctcaggagtg gaccgacgac ctcgtgaggc tgcgcgagcg ctacccctgg 1981 ctcgtcgctg aggtggatgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggta 2041 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa 2101 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag 2161 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat 2221 gtgcaggcac gcaacgccta cgactggacg gcggagagca ccgtctacgt gtccccacgc 2281 caccagcgca ccggcctggg ctccacgctc tacacccacc tgctcaagag cctggaggcc 2341 cagggcttca agtccgtggt cgctgtgatc ggcctcccca acgacccgag cgtccgtatg 2401 cacgaggccc tcggctacgc gccccgcggg atgctgaggg ccgcgggctt caagcacggc 2461 aactggcacg acgtgggctt ctggcagctc gacttctccc tgcctgtccc accccgcccg 2521 gtgctccccg tcaccgaaat ctgaacgcgt tcgagtatta tggcattggg aaaactgttt 2581 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg 2641 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt 2701 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa 2761 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg 2821 aagttaatat gaggagtaaa acactgaagc ctgcaggcat gcaagctgat ccactagagg 2881 ccatggcggc cgcactagtg gatcccccgg gctgcaggaa ttcaagctta cgcgtcctcc 2941 ataactatgg gcaagggcga ggaactgttc actggcgtgg tcccaatcct ggtggaactg 3001 gatggtgatg tgaacgggca caagttctcc gtcagcggag agggtgaagg tgatgccacc 3061 tacggaaagc tcaccctgaa gttcatctgc actaccggaa agctccctgt tccgtggcca 3121 accctcgtca ccactttcac ctacggtgtt cagtgcttct cccggtaccc agatcacatg 3181 aagcagcatg acttcttcaa gagcgccatg cccgaaggct acgtgcaaga aaggactatc 3241 ttcttcaagg atgacgggaa ctacaagaca cgtgccgaag tcaagttcga aggtgatacc 3301 ctggtgaacc gcatcgagct gaaaggtaag tttctgcttc tacctttgat atatatataa 3361 taattatcat taattagtag taatataata tttcaaatat ttttttcaaa ataaaagaat 3421 gtagtatata gcaattgctt ttctgtagtt tataagtgtg tatattttaa tttataactt 3481 ttctaatata tgaccaaaat ttgttgatgt gcaggtatcg atttcaagga agatggaaac 3541 atcctcggac acaagctgga gtacaactac aactcccaca acgtatacat catggccgac 3601 aagcagaaga acggcatcaa ggtgaacttc aagatcaggc acaacatcga agatggaagc 3661 gtgcaactgg cggaccacta ccagcagaac acgcccatcg gcgatggccc tgtcctgctg 3721 ccggacaacc attacctgtc cacgcaatct gccctctcca aggaccccaa cgagaagagg 3781 gaccacatgg tcctgctgga gttcgtgacg gctgctggga tcacgcatgg catggatgaa 3841 ctctacaagt gactcgtcga ccctgcttta atgagatatg cgagacgcct atgatcgcat 3901 gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga 3961 tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt 4021 atgaataata ttctccgttc aatttactga ttgtcctcga caggccttaa gggccagatc 4081 ttgggcccgg tacccgatca gattgtcgtt tcccgccttc ggtttaaact atcagtgttt 4141 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata 4201 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag 4261 ggttcccctc gggagtgctt ggcattccgt gcgataatga cttctgttca accacccaaa 4321 cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat cccttggctc gtctgggtcg 4381 gctagaaggt cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc 4441 agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg 4501 ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac aggatgatct 4561 ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg gcgggaactc cacgaaaata 4621 tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc gccgccgacg 4681 ccgttgatgt ggacgccgaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 4741 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 4801 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 4861 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 4921 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 4981 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 5041 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 5101 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 5161 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 5221 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 5281 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 5341 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 5401 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 5461 tttcctgcgt tatcccctga ttctgtggat aaccgattac cgcctttgag tgagctgata 5521 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 5581 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca 5641 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct 5701 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg 5761 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 5821 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg caggggtacg tcgaggtcga 5881 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg 5941 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct 6001 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga 6061 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca 6121 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc 6181 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg 6241 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca 6301 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc 6361 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg 6421 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg 6481 ccgccaaggc gcgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg 6541 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc 6601 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca 6661 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg 6721 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga 6781 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt 6841 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga 6901 tgccaagctc gcggcctggc cggcgagctt ggccgctgaa gaaaccgagc gccgccgtct 6961 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat 7021 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac 7081 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc 7141 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg 7201 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc 7261 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg 7321 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc 7381 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc 7441 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc 7501 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc 7561 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa 7621 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc 7681 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc 7741 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca 7801 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg 7861 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt 7921 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa 7981 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt 8041 tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag 8101 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc 8161 tgggtgatga cctggtggag aagttgaagg cggcgcaggc cgcccagcgg caacgcatcg 8221 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat 8281 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc 8341 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca 8401 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct 8461 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gcgagtgtgt 8521 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc 8581 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca 8641 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc 8701 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg 8761 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg 8821 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag 8881 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg 8941 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt 9001 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg 9061 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc 9121 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg 9181 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa 9241 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc 9301 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga 9361 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa 9421 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc 9481 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta 9541 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg 9601 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ggtgggtatg 9661 cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct 9721 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac 9781 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct 9841 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt 9901 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc 9961 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc 10021 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat 10081 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg 10141 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt 10201 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc 10261 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca 10321 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg 10381 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg 10441 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg 10501 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct 10561 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt 10621 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg 10681 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg 10741 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg 10801 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt 10861 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa 10921 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata 10981 agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt tatcgcaata 11041 gttggcgaag taatcgcaac atagcttgct tggtcgttcc gcgtgaacgt cggctcgatt 11101 gtacctgcgt tcaaatactt tgcgatcgtg ttgcgcgcct gcccggtgcg tcggctgatc 11161 tcacggatcg actgcttctc tcgcaacgcc atccgacgga tgatgtttaa aagtcccatg 11221 tggatcactc cgttgccccg tcgctcaccg tgttgggggg aaggtgcaca tggctcagtt 11281 ctcaatggaa attatctgcc taaccggctc agttctgcgt agaaaccaac atgcaagctc 11341 caccgggtgc aaagcggcag cggc //