LOCUS B438p9oU10-oGFPi 11616 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of B444pmGFPD-ocs* into B435p9oU10-LH* ACCESSION B438p9oU10-oGFPi KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11616) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B435p9oU10-LH* Fragment ends: SfiI Fragment size: 10432 Insert molecule: B444pmGFPD-ocs* Fragment ends: SfiI Fragment size: 1184 Sequencing primers GFP-seq CCAGTTCCACCAGGATTG SphR GGGTGGTTGAACAGAAGTCAT Maxi FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1476 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2102 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 2099..2102 /label=splice /SECDrawAs="Label" CDS 2103..2289 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 2288..2291 /label=splice /SECDrawAs="Label" CDS 2290..2793 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2807..3097 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 CDS 3199..3577 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 3578..3766 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 3767..4104 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 4113..4308 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 4393..4416 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4951..5648 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5685..5944 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 6197..9902 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 10086..11096 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 actatgggag catggattga gcaggatggc ctccatgctg gtagccccgc tgcttgggtg 1861 gagcgcctct ttggctacga ctgggcccaa cagaccattg ggtgcagtga tgccgctgtc 1921 ttccgtttga gcgcccaggg caggccagtt ctcttcgtga agactgacct ctccggtgcc 1981 ctcaacgagt tgcaggacga agctgcccgc ctctcatggc ttgccaccac cggagtccct 2041 tgcgctgccg tgttggacgt tgtcacagag gccggaagag actggctgct cttgggcgag 2101 gtaagtttct gcttctacct ttgatatata tataataatt atcattaatt agtagtaata 2161 taatatttca aatatttttt tcaaaataaa agaatgtagt atatagcaat tgcttttctg 2221 tagtttataa gtgtgtatat tttaatttat aacttttcta atatatgacc aaaatttgtt 2281 gatgtgcagg tgccaggcca agacctcctg agctcccacc tggccccagc cgagaaggtc 2341 tccatcatgg cagacgccat gcgtcgcctc cacaccctgg acccagccac ctgccccttc 2401 gaccaccagg ccaagcacag gatcgaacgt gcacgcacta ggatggaggc cggccttgtg 2461 gaccaggatg accttgacga ggagcaccag ggccttgctc ccgccgagct gttcgcccgc 2521 ctcaaggcta gaatgcctga cggagaggac ttggtggtca cccacgggga tgcctgcctc 2581 cccaacatca tggttgagaa cggacgcttc tctggcttca tcgactgcgg tcgtctgggc 2641 gtggccgacc gctaccaaga catcgccctg gccaccaggg acatcgctga ggaacttgga 2701 ggggagtggg ccgatcgttt cttggtcctc tacggcattg ctgcccccga ctcccagagg 2761 atcgccttct accgcctgct tgacgagttc ttctgaacgc gttcgagtat tatggcattg 2821 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg 2881 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc 2941 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat 3001 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc 3061 ctctaatgac cgaagttaat atgaggagta aaacactgaa gcctgcaggc atgcaagctg 3121 atccactaga ggccatggcg gccgcactag tggatccccc gggctgcagg aattcaagct 3181 tacgcgtcct ccataactat gggcaagggc gaggaactgt tcactggcgt ggtcccaatc 3241 ctggtggaac tggatggtga tgtgaacggg cacaagttct ccgtcagcgg agagggtgaa 3301 ggtgatgcca cctacggaaa gctcaccctg aagttcatct gcactaccgg aaagctccct 3361 gttccgtggc caaccctcgt caccactttc acctacggtg ttcagtgctt ctcccggtac 3421 ccagatcaca tgaagcagca tgacttcttc aagagcgcca tgcccgaagg ctacgtgcaa 3481 gaaaggacta tcttcttcaa ggatgacggg aactacaaga cacgtgccga agtcaagttc 3541 gaaggtgata ccctggtgaa ccgcatcgag ctgaaaggta agtttctgct tctacctttg 3601 atatatatat aataattatc attaattagt agtaatataa tatttcaaat atttttttca 3661 aaataaaaga atgtagtata tagcaattgc ttttctgtag tttataagtg tgtatatttt 3721 aatttataac ttttctaata tatgaccaaa atttgttgat gtgcaggtat cgatttcaag 3781 gaagatggaa acatcctcgg acacaagctg gagtacaact acaactccca caacgtatac 3841 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatcag gcacaacatc 3901 gaagatggaa gcgtgcaact ggcggaccac taccagcaga acacgcccat cggcgatggc 3961 cctgtcctgc tgccggacaa ccattacctg tccacgcaat ctgccctctc caaggacccc 4021 aacgagaaga gggaccacat ggtcctgctg gagttcgtga cggctgctgg gatcacgcat 4081 ggcatggatg aactctacaa gtgactcgtc gaccctgctt taatgagata tgcgagacgc 4141 ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat 4201 gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat cacccgttac 4261 tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtcctc gacaggcctt 4321 aagggccaga tcttgggccc ggtacccgat cagattgtcg tttcccgcct tcggtttaaa 4381 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag 4441 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca 4501 tgccaaccac agggttcccc tcgggagtgc ttggcattcc gtgcgataat gacttctgtt 4561 caaccaccca aacgtcggaa agcctgacga cggagcagca ttccaaaaag atcccttggc 4621 tcgtctgggt cggctagaag gtcgagtggg ctgctgtggc ttgatccctc aacgcggtcg 4681 cggacgtagc gcagcgccga aaaatcctcg atcgcaaatc cgacgctgtc gaaaagcgtg 4741 atctgcttgt cgctctttcg gccgacgtcc tggccagtca tcacgcgcca aagttccgtc 4801 acaggatgat ctggcgcgag ttgctggatc tcgccttcaa tccgggtctg tggcgggaac 4861 tccacgaaaa tatccgaacg cagcaagata tcgcggtgca tctcggtctt gcctgggcag 4921 tcgccgccga cgccgttgat gtggacgccg aaaaggatct aggtgaagat cctttttgat 4981 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 5041 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 5101 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 5161 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 5221 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 5281 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5341 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5401 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 5461 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5521 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 5581 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 5641 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 5701 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgatt accgcctttg 5761 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 5821 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 5881 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac 5941 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga 6001 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 6061 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggggta 6121 cgtcgaggtc gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc 6181 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc 6241 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg 6301 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg 6361 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg 6421 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg 6481 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc 6541 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg 6601 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt 6661 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga 6721 gcgggcgcga ggccgccaag gcgcgaggcg tgaagtttgg cccccgccct accctcaccc 6781 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg 6841 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg 6901 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg 6961 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca 7021 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc 7081 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc 7141 cggtttgtct gatgccaagc tcgcggcctg gccggcgagc ttggccgctg aagaaaccga 7201 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc 7261 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc 7321 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc 7381 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc 7441 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg 7501 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg 7561 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg 7621 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag 7681 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa 7741 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt 7801 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt 7861 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt 7921 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc 7981 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag 8041 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga 8101 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc 8161 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga 8221 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga 8281 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact 8341 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa 8401 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggcggcgcag gccgcccagc 8461 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa 8521 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca 8581 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata 8641 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg 8701 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca 8761 tggcgagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca 8821 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg 8881 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag 8941 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga 9001 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa 9061 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg 9121 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg 9181 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag 9241 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt 9301 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg 9361 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg 9421 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg 9481 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt 9541 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac 9601 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa 9661 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag 9721 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt 9781 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc 9841 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca 9901 ccggtgggta tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg 9961 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac 10021 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt 10081 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg 10141 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg 10201 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg 10261 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga 10321 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac 10381 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca 10441 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt 10501 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg 10561 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg 10621 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca 10681 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg 10741 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc 10801 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg 10861 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg 10921 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg 10981 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt 11041 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc 11101 taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca 11161 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa 11221 gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt 11281 gttatcgcaa tagttggcga agtaatcgca acatagcttg cttggtcgtt ccgcgtgaac 11341 gtcggctcga ttgtacctgc gttcaaatac tttgcgatcg tgttgcgcgc ctgcccggtg 11401 cgtcggctga tctcacggat cgactgcttc tctcgcaacg ccatccgacg gatgatgttt 11461 aaaagtccca tgtggatcac tccgttgccc cgtcgctcac cgtgttgggg ggaaggtgca 11521 catggctcag ttctcaatgg aaattatctg cctaaccggc tcagttctgc gtagaaacca 11581 acatgcaagc tccaccgggt gcaaagcggc agcggc //