LOCUS E040pHSP-mGFPM-o 4510 bp DNA circular SYN 13-JUL-2020 DEFINITION Ligation of B594p2x35s-mGFPM-ocs* into A212pHSP-DGA* ACCESSION E040pHSP-mGFPM-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4510) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: A212pHSP-DGA* Fragment ends: SalI and HindIII Fragment size: 3578 Insert molecule: B594p2x35s-mGFPM-ocs* Fragment ends: HindIII and SalI Fragment size: 932 Sequencing primers HSP-SeqF GTGTCCTTTGAAGACACATCAC FEATURES Location/Qualifiers misc_feature 100..530 /gene="HSP" /product="Promoter" /SECDrawAs="Region" /SECStyleId=1 CDS 585..963 /gene="GFP" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 964..1152 /gene="ST-LS1" /product="Intron" /SECDrawAs="Gene" /SECStyleId=1 CDS 1153..1490 /gene="GFP" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1499..1694 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="TOCS" /SECDescr="Terminator" misc_feature 2510..3370 /gene="Amp" /product="ORF-1" /SECDrawAs="Region" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" misc_feature 3413..4129 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca gtcagccttt taagagatag 121 aatttaaaat ataatttgcg taaaacatta ttaaaaatac aaatttataa attaagttca 181 actcatccta tctcactctt taaatacgat gtttacttat tagactcatt aataaaaaaa 241 aaaaaaatca tttgtacaaa gcccaccata aaggcaattt gggcctggta gaccaatcct 301 aaccaatgtc tggttaagat ggtccaatcc cgaaacttct agttgcggtt cgaagaagtc 361 cagaatgttt ctgaaagttt cagaaaattc tagttttgag attttcagaa gtacggcatg 421 atgatgcata acaaggactt tctcgaaagt actatattgc tcctctacat cattttaaat 481 accccatgtg tcctttgaag acacatcaca gaaagaagtg aaggcatcgt actagttgga 541 tcccccgggc tgcaggaatt caagcttacg cgtcctccac cgaaatgggc aagggcgagg 601 aactgttcac tggcgtggtc ccaatcctgg tggaactgga tggtgatgtg aacgggcaca 661 agttctccgt cagcggagag ggtgaaggtg atgccaccta cggaaagctc accctgaagt 721 tcatctgcac taccggaaag ctccctgttc cgtggccaac cctcgtcacc actttcacct 781 acggtgttca gtgcttctcc cggtacccag atcacatgaa gcagcatgac ttcttcaaga 841 gcgccatgcc cgaaggctac gtgcaagaaa ggactatctt cttcaaggat gacgggaact 901 acaagacacg tgccgaagtc aagttcgaag gtgataccct ggtgaaccgc atcgagctga 961 aaggtaagtt tctgcttcta cctttgatat atatataata attatcatta attagtagta 1021 atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt 1081 ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt 1141 gttgatgtgc aggtatcgat ttcaaggaag atggaaacat cctcggacac aagctggagt 1201 acaactacaa ctcccacaac gtatacatca tggccgacaa gcagaagaac ggcatcaagg 1261 tgaacttcaa gatcaggcac aacatcgaag atggaagcgt gcaactggcg gaccactacc 1321 agcagaacac gcccatcggc gatggccctg tcctgctgcc ggacaaccat tacctgtcca 1381 cgcaatctgc cctctccaag gaccccaacg agaagaggga ccacatggtc ctgctggagt 1441 tcgtgacggc tgctgggatc acgcatggca tggatgaact ctacaagtga ctcgtcgacc 1501 ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt 1561 gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc 1621 attctaatga atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa 1681 tttactgatt gtcctcgaca ggccttaagg gccagatctt gggcccggta cccaattcgc 1741 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa 1801 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 1861 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 1921 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 1981 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 2041 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 2101 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 2161 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 2221 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 2281 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 2341 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa 2401 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 2461 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 2521 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 2581 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 2641 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 2701 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 2761 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 2821 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 2881 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 2941 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 3001 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 3061 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 3121 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 3181 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 3241 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 3301 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 3361 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 3421 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 3481 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 3541 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 3601 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 3661 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 3721 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 3781 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3841 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 3901 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 3961 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 4021 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 4081 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 4141 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 4201 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 4261 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 4321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 4381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 4441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 4501 ataacaattt //