LOCUS B595pUbiZm-mGFPM 5580 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of B442pmGFPM-ocs* into B497pUbiZm-MoGusi-ocs* ACCESSION B595pUbiZm-mGFPM KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5580) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B497pUbiZm-MoGusi-ocs* Fragment ends: SalI and HindIII Fragment size: 4648 Insert molecule: B442pmGFPM-ocs* Fragment ends: HindIII and SalI Fragment size: 932 GELR Sequencing primers OR2 GTGCACAACAGAATTGAAAGCAAA weglassen UF2 FEATURES Location/Qualifiers misc_feature 122..1629 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1108..1627 /gene="int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 CDS 1655..2033 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 2034..2222 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 2223..2560 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2569..2764 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3580..4440 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 4483..5199 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagtggatc ccccgggtac 121 gtgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 181 agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 241 tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 301 tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 361 gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 421 ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 481 gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 541 agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 601 taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 661 aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 721 tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag 781 cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg 841 ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg 901 gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc 961 caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc 1021 ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa 1081 tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc 1141 taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc 1201 atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg 1261 cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc 1321 ctgggatggc tctagccgtt ccgcagacgg gatcgatcta ggataggtat acatgttgat 1381 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac 1441 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat 1501 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg 1561 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact 1621 tctgcaggta caagcttacg cgtcctccac cgaaatgggc aagggcgagg aactgttcac 1681 tggcgtggtc ccaatcctgg tggaactgga tggtgatgtg aacgggcaca agttctccgt 1741 cagcggagag ggtgaaggtg atgccaccta cggaaagctc accctgaagt tcatctgcac 1801 taccggaaag ctccctgttc cgtggccaac cctcgtcacc actttcacct acggtgttca 1861 gtgcttctcc cggtacccag atcacatgaa gcagcatgac ttcttcaaga gcgccatgcc 1921 cgaaggctac gtgcaagaaa ggactatctt cttcaaggat gacgggaact acaagacacg 1981 tgccgaagtc aagttcgaag gtgataccct ggtgaaccgc atcgagctga aaggtaagtt 2041 tctgcttcta cctttgatat atatataata attatcatta attagtagta atataatatt 2101 tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt ctgtagttta 2161 taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt gttgatgtgc 2221 aggtatcgat ttcaaggaag atggaaacat cctcggacac aagctggagt acaactacaa 2281 ctcccacaac gtatacatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 2341 gatcaggcac aacatcgaag atggaagcgt gcaactggcg gaccactacc agcagaacac 2401 gcccatcggc gatggccctg tcctgctgcc ggacaaccat tacctgtcca cgcaatctgc 2461 cctctccaag gaccccaacg agaagaggga ccacatggtc ctgctggagt tcgtgacggc 2521 tgctgggatc acgcatggca tggatgaact ctacaagtga ctcgtcgacc ctgctttaat 2581 gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg 2641 taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga 2701 atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt 2761 gtcctcgaca ggccttaagg gccagatctt gggcccggta cccaattcgc cctatagtga 2821 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt 2881 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga 2941 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta 3001 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 3061 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 3121 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 3181 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 3241 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 3301 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 3361 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 3421 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga 3481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 3541 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 3601 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 3661 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 3721 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 3781 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 3841 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 3901 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 3961 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 4021 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 4081 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 4141 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 4201 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 4261 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 4321 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 4381 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 4441 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 4501 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 4561 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 4621 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 4681 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 4741 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 4801 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 4861 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 4921 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 4981 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 5041 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 5101 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 5161 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 5221 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 5281 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 5341 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 5401 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 5461 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 5521 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt //