LOCUS B635pActOs-LGFP 5526 bp DNA circular SYN 08-FEB-2016 DEFINITION Ligation of B629p35s-LGFP* into B627pAct-ocs* ACCESSION B635pActOs-LGFP KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5526) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B627pAct-ocs* Fragment ends: KpnI and XmaI Fragment size: 4292 Insert molecule: B629p35s-LGFP* Fragment ends: XmaI and KpnI Fragment size: 1234 Sequencing primers F3F CAGCATTGTTCATCGGTAGT pUCF GCAAGGCGATTAAGTTGGGTA FEATURES Location/Qualifiers misc_feature 103..1500 /gene="ACT" /product="Actin Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 946..1500 /gene="mRNA" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1036..1497 /gene="int" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1549..1599 /gene="link" /product="linker" /SECDrawAs="Region" /SECStyleId=1 CDS 1600..1978 /gene="gfpm" /product="GFPm" /SECDrawAs="Gene" /SECStyleId=1 CDS 1979..2167 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 2168..2505 /gene="gfpm" /product="GFPm" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2515..2710 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="OCS" /SECDescr="Terminator" misc_feature 3526..4386 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" misc_feature 4429..5145 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ggtcgaggtc attcatatgc 121 ttgagaagag agtcgggata gtccaaaata aaacaaaggt aagattacct ggtcaaaagt 181 gaaaacatca gttaaaaggt ggtataaagt aaaatatcgg taataaaagg tggcccaaag 241 tgaaatttac tcttttctac tattataaaa attgaggatg tttttgtcgg tactttgata 301 cgtcattttt gtatgaattg gtttttaagt ttattcgctt ttggaaatgc atatctgtat 361 ttgagtcggg ttttaagttc gtttgctttt gtaaatacag agggatttgt ataagaaata 421 tctttaaaaa aacccatatg ctaatttgac ataatttttg agaaaaatat atattcaggc 481 gaattctcac aatgaacaat aataagatta aaatagcttt cccccgttgc agcgcatggg 541 tattttttct agtaaaaata aaagataaac ttagactcaa aacatttaca aaaacaaccc 601 ctaaagttcc taaagcccaa agtgctatcc acgatccata gcaagcccag cccaacccaa 661 cccaacccaa cccaccccag tccagccaac tggacaatag tctccacacc cccccactat 721 caccgtgagt tgtccgcacg caccgcacgt ctcgcagcca aaaaaaaaaa aagaaagaaa 781 aaaaagaaaa agaaaaaaca gcaggtgggt ccgggtcgtg ggggccggaa acgcgaggag 841 gatcgcgagc cagcgacgag gccggccctc cctccgcttc caaagaaacg ccccccatcg 901 ccactatata catacccccc cctctcctcc catcccccca accctaccac caccaccacc 961 accacctcca cctcctcccc cctcgctgcc ggacgacgag ctcctccccc ctccccctcc 1021 gccgccgccg cgccggtaac caccccgccc ctctcctctt tctttctccg tttttttttt 1081 ccgtctcggt ctcgatcttt ggccttggta gtttgggtgg gcgagaggcg gcttcgtgcg 1141 cgcccagatc ggtgcgcggg aggggcggga tctcgcggct ggggctctcg ccggcgtgga 1201 tccggcccgg atctcgcggg gaatggggct ctcggatgta gatctgcgat ccgccgttgt 1261 tgggggagat gatggggggt ttaaaatttc cgccatgcta aacaagatca ggaagagggg 1321 aaaagggcac tatggtttat atttttatat atttctgctg cttcgtcagg cttagatgtg 1381 ctagatcttt ctttcttctt tttgtgggta gaatttgaat ccctcagcat tgttcatcgg 1441 tagtttttct tttcatgatt tgtgacaaat gcagcctcgt gcggagcttt tttgtaggta 1501 ctagttggat cccccgggct gcaggaattc aagcttacgc gttgtacagg agtaccgccc 1561 cgtccggtcc tgcccgtcac cgagatcagc ggagagttca tgggcaaggg cgaggaactg 1621 ttcactggcg tggtcccaat cctggtggaa ctggatggtg atgtgaacgg gcacaagttc 1681 tccgtcagcg gagagggtga aggtgatgcc acctacggaa agctcaccct gaagttcatc 1741 tgcactaccg gaaagctccc tgttccgtgg ccaaccctcg tcaccacttt cacctacggt 1801 gttcagtgct tctcccgtta cccagatcac atgaagcagc atgacttctt caagagcgcc 1861 atgcccgaag gctacgtgca agaaaggact atcttcttca aggatgacgg gaactacaag 1921 acacgtgccg aagtcaagtt cgaaggtgat accctggtga accgcatcga gctgaaaggt 1981 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata 2041 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta 2101 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga 2161 tgtgcaggta tcgatttcaa ggaagatgga aacatcctcg gacacaagct ggagtacaac 2221 tacaactccc acaacgtata catcatggcc gacaagcaga agaacggcat caaggtgaac 2281 ttcaagatca ggcacaacat cgaagatgga agcgtgcaac tggcggacca ctaccagcag 2341 aacacgccca tcggcgatgg ccctgtcctg ctgccggaca accattacct gtccacgcaa 2401 tctgccctct ccaaggaccc caacgagaag agggaccaca tggtcctgct ggagttcgtg 2461 acggctgctg ggatcacgca tggcatggat gaactctaca agtgacccgg tcgaccctgc 2521 tttaatgaga tatgcgagac gcctatgatc gcatgatatt tgctttcaat tctgttgtgc 2581 acgttgtaaa aaacctgagc atgtgtagct cagatcctta ccgccggttt cggttcattc 2641 taatgaatat atcacccgtt actatcgtat ttttatgaat aatattctcc gttcaattta 2701 ctgattgtcc tcgacaggcc ttaagggcca gatcttgggc ccggtaccca attcgcccta 2761 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 2821 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 2881 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa 2941 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt 3001 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata 3061 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac 3121 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa 3181 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc 3241 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg 3301 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca 3361 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg 3421 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 3481 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 3541 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 3601 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 3661 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 3721 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 3781 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 3841 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 3901 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 3961 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 4021 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 4081 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 4141 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 4201 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 4261 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 4321 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 4381 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 4441 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 4501 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 4561 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 4621 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 4681 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 4741 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 4801 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 4861 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 4921 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 4981 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 5041 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 5101 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 5161 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 5221 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 5281 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 5341 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 5401 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 5461 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 5521 caattt //