LOCUS B444pmGFPD-ocs 4078 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of GFPdi-Hind-Sal* into pOCS-BM* ACCESSION B444pmGFPD-ocs KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4078) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pOCS-BM* Fragment ends: SalI and HindIII Fragment size: 3146 Insert molecule: GFPdi-Hind-Sal* Fragment ends: HindIII and SalI Fragment size: 932 Primer for gene synthesis DHindGFP cccgtcAAGCTTACGCGtcctccataactATGGGCAAGGGC SalGFP CCCGTGgtcgaCGAGTCACTTGTAGAG Sequencing primers OR2 GTGCACAACAGAATTGAAAGCAAA FEATURES Location/Qualifiers CDS 81..459 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 460..648 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 649..986 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 995..1190 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2006..2866 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 2909..3625 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gagctctcta gaggccatgg cggccgcact agtggatccc ccgggctgca ggaattcaag 61 cttacgcgtc ctccataact atgggcaagg gcgaggaact gttcactggc gtggtcccaa 121 tcctggtgga actggatggt gatgtgaacg ggcacaagtt ctccgtcagc ggagagggtg 181 aaggtgatgc cacctacgga aagctcaccc tgaagttcat ctgcactacc ggaaagctcc 241 ctgttccgtg gccaaccctc gtcaccactt tcacctacgg tgttcagtgc ttctcccggt 301 acccagatca catgaagcag catgacttct tcaagagcgc catgcccgaa ggctacgtgc 361 aagaaaggac tatcttcttc aaggatgacg ggaactacaa gacacgtgcc gaagtcaagt 421 tcgaaggtga taccctggtg aaccgcatcg agctgaaagg taagtttctg cttctacctt 481 tgatatatat ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt 541 caaaataaaa gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt 601 ttaatttata acttttctaa tatatgacca aaatttgttg atgtgcaggt atcgatttca 661 aggaagatgg aaacatcctc ggacacaagc tggagtacaa ctacaactcc cacaacgtat 721 acatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc aggcacaaca 781 tcgaagatgg aagcgtgcaa ctggcggacc actaccagca gaacacgccc atcggcgatg 841 gccctgtcct gctgccggac aaccattacc tgtccacgca atctgccctc tccaaggacc 901 ccaacgagaa gagggaccac atggtcctgc tggagttcgt gacggctgct gggatcacgc 961 atggcatgga tgaactctac aagtgactcg tcgaccctgc tttaatgaga tatgcgagac 1021 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc 1081 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt 1141 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtcc tcgacaggcc 1201 ttaagggcca gatcttgggc ccggtaccca attcgcccta tagtgagtcg tattacaatt 1261 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 1321 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc 1381 gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt 1441 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 1501 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 1561 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 1621 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 1681 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 1741 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 1801 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 1861 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 1921 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 1981 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 2041 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 2101 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 2161 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 2221 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 2281 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 2341 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 2401 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 2461 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 2521 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 2581 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 2641 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 2701 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 2761 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 2821 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 2881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 2941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 3001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 3061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 3121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 3181 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 3241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 3301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 3361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 3421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 3481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 3541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 3601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 3661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 3721 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 3781 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 3841 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 3901 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 3961 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 4021 ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctg //