LOCUS B589p35s-mGFPD-o 4489 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of B444pmGFPD-ocs* into B491p35s-DoGusi-ocs* ACCESSION B589p35s-mGFPD-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4489) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B491p35s-DoGusi-ocs* Fragment ends: SalI and HindIII Fragment size: 3557 Insert molecule: B444pmGFPD-ocs* Fragment ends: HindIII and SalI Fragment size: 932 GELR Sequencing primers OR2 GTGCACAACAGAATTGAAAGCAAA FEATURES Location/Qualifiers misc_feature 144..562 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" misc_signal 473..562 /label=Minimal promotor /SECDrawAs="Label" misc_feature 563..564 /product="T-start" /SECDrawAs="Region" /SECStyleId=1 CDS 590..968 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 TATA_signal 969..1157 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 1158..1495 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1504..1699 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2515..3375 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 3418..4134 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg 121 gccgcactag tggatccccc gggtcaacat ggtggagcac gacactctcg tctactccaa 181 gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt 241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac 301 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt 361 tcaagatgcc tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt 421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac 481 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg 541 aagttcattt catttggaga ggacacaagc ttacgcgtcc tccataacta tgggcaaggg 601 cgaggaactg ttcactggcg tggtcccaat cctggtggaa ctggatggtg atgtgaacgg 661 gcacaagttc tccgtcagcg gagagggtga aggtgatgcc acctacggaa agctcaccct 721 gaagttcatc tgcactaccg gaaagctccc tgttccgtgg ccaaccctcg tcaccacttt 781 cacctacggt gttcagtgct tctcccggta cccagatcac atgaagcagc atgacttctt 841 caagagcgcc atgcccgaag gctacgtgca agaaaggact atcttcttca aggatgacgg 901 gaactacaag acacgtgccg aagtcaagtt cgaaggtgat accctggtga accgcatcga 961 gctgaaaggt aagtttctgc ttctaccttt gatatatata taataattat cattaattag 1021 tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg 1081 cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa 1141 aatttgttga tgtgcaggta tcgatttcaa ggaagatgga aacatcctcg gacacaagct 1201 ggagtacaac tacaactccc acaacgtata catcatggcc gacaagcaga agaacggcat 1261 caaggtgaac ttcaagatca ggcacaacat cgaagatgga agcgtgcaac tggcggacca 1321 ctaccagcag aacacgccca tcggcgatgg ccctgtcctg ctgccggaca accattacct 1381 gtccacgcaa tctgccctct ccaaggaccc caacgagaag agggaccaca tggtcctgct 1441 ggagttcgtg acggctgctg ggatcacgca tggcatggat gaactctaca agtgactcgt 1501 cgaccctgct ttaatgagat atgcgagacg cctatgatcg catgatattt gctttcaatt 1561 ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc 1621 ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg 1681 ttcaatttac tgattgtcct cgacaggcct taagggccag atcttgggcc cggtacccaa 1741 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg 1801 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 1861 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 1921 cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc 1981 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag 2041 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg 2101 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca 2161 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa 2221 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg 2281 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta 2341 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg 2401 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 2461 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 2521 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 2581 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 2641 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 2701 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 2761 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 2821 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 2881 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 2941 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 3001 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 3061 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 3121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 3181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 3241 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 3301 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 3361 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 3421 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 3481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 3541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 3601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 3661 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 3721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 3781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 3841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 3901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 3961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 4021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 4081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 4141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 4201 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 4261 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 4321 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 4381 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 4441 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgt //