LOCUS B591pUbiHa-mGFPD 4406 bp DNA circular SYN 08-FEB-2016 DEFINITION Ligation of B444pmGFPD-ocs* into B493pUbiHa-DoGusi-ocs* ACCESSION B591pUbiHa-mGFPD KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4406) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B493pUbiHa-DoGusi-ocs* Fragment ends: SalI and HindIII Fragment size: 3474 Insert molecule: B444pmGFPD-ocs* Fragment ends: HindIII and SalI Fragment size: 932 GELR Sequencing primers: Xba nur Mini T7QL nur Maxi FEATURES Location/Qualifiers misc_feature 146..481 /gene="UbiHa" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_signal 263..265 /label=cat /SECDrawAs="Label" misc_signal 266..268 /label=cat /SECDrawAs="Label" misc_signal 299..301 /label=cat /SECDrawAs="Label" misc_signal 328..330 /label=cat /SECDrawAs="Label" misc_signal 339..341 /label=cat /SECDrawAs="Label" misc_signal 360..362 /label=cat /SECDrawAs="Label" misc_signal 363..365 /label=cat /SECDrawAs="Label" misc_signal 368..371 /label=tata /SECDrawAs="Label" CDS 399..481 /gene="mRNA" /SECDrawAs="Gene" /SECStyleId=1 CDS 507..885 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 886..1074 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 1075..1412 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1421..1616 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2432..3292 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 3335..4051 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg 121 gccgcactag tggatccccc gggacaaaac ttaagaaagt caccaaaaag aaagtcaaag 181 acggcaacgg caacccagaa gctacgttct ctttctagat tctatgccct acctaccgcg 241 tggtgcacaa caacttgacc cccatcattg actctccacc gtacacgtgt cacgatacca 301 tttgttggtc tcctaccact tacgtaacat taatattcca tccgtgaccg cctcttcttc 361 atcatcctat aaattcacct tcttatctct tccgctttca ttcgttcatc atcatcacaa 421 ttaacaacaa tccaaagcaa tcttctctca tattcttatc tcttcctgtt tcgcttcgtc 481 tacaagctta cgcgtcctcc ataactatgg gcaagggcga ggaactgttc actggcgtgg 541 tcccaatcct ggtggaactg gatggtgatg tgaacgggca caagttctcc gtcagcggag 601 agggtgaagg tgatgccacc tacggaaagc tcaccctgaa gttcatctgc actaccggaa 661 agctccctgt tccgtggcca accctcgtca ccactttcac ctacggtgtt cagtgcttct 721 cccggtaccc agatcacatg aagcagcatg acttcttcaa gagcgccatg cccgaaggct 781 acgtgcaaga aaggactatc ttcttcaagg atgacgggaa ctacaagaca cgtgccgaag 841 tcaagttcga aggtgatacc ctggtgaacc gcatcgagct gaaaggtaag tttctgcttc 901 tacctttgat atatatataa taattatcat taattagtag taatataata tttcaaatat 961 ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt tataagtgtg 1021 tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt gcaggtatcg 1081 atttcaagga agatggaaac atcctcggac acaagctgga gtacaactac aactcccaca 1141 acgtatacat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatcaggc 1201 acaacatcga agatggaagc gtgcaactgg cggaccacta ccagcagaac acgcccatcg 1261 gcgatggccc tgtcctgctg ccggacaacc attacctgtc cacgcaatct gccctctcca 1321 aggaccccaa cgagaagagg gaccacatgg tcctgctgga gttcgtgacg gctgctggga 1381 tcacgcatgg catggatgaa ctctacaagt gactcgtcga ccctgcttta atgagatatg 1441 cgagacgcct atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac 1501 ctgagcatgt gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca 1561 cccgttacta tcgtattttt atgaataata ttctccgttc aatttactga ttgtcctcga 1621 caggccttaa gggccagatc ttgggcccgg tacccaattc gccctatagt gagtcgtatt 1681 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 1741 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 1801 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa 1861 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 1921 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 1981 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 2041 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 2101 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 2161 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 2221 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 2281 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 2341 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 2401 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 2461 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 2521 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 2581 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 2641 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 2701 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 2761 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 2821 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 2881 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 2941 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 3001 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 3061 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 3121 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 3181 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 3241 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 3301 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 3361 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 3421 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 3481 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 3541 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 3601 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 3661 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 3721 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 3781 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 3841 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 3901 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 3961 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4021 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4081 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 4141 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 4201 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 4261 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 4321 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 4381 ctttatgctt ccggctcgta tgttgt //