LOCUS B592pUbiAt-mGFPD 5544 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of B494pUbiAt-DoGusi-ocs* into B444pmGFPD-ocs* ACCESSION B592pUbiAt-mGFPD KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5544) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B444pmGFPD-ocs* Fragment ends: HindIII and BamHI Fragment size: 4054 Insert molecule: B494pUbiAt-DoGusi-ocs* Fragment ends: BamHI and HindIII Fragment size: 1490 GELR Sequencing primers M13RY CACACAGGAAACAGCTATGAC GFP-seq CCAGTTCCACCAGGATTG FEATURES Location/Qualifiers misc_feature 46..1521 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1150..1213 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1216..1519 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1547..1925 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 1926..2114 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 2115..2452 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2461..2656 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3472..4332 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 4375..5091 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gagctctcta gaggccatgg cggccgcact agtggatccc ccgggtttcc ttacattctg 61 agcctctttc cttctaatcc actcatctgc atcttcttgt gtccttacta atacctcatt 121 ggttccaaat tccctccctt taagcaccag ctcgtttctg ttcttccaca gcctcccaag 181 tatccaaggg actaaagcct ccacattctt cagatcagga tattcttgtt taagatgttg 241 aactctatgg aggtttgtat gaactgatga tctaggaccg gataagttcc cttcttcata 301 gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt acattgttat taatgaaaaa 361 atattattgg tcattggact gaacacgagt gttaaatatg gaccaggccc caaataagat 421 ccattgatat atgaattaaa taacaagaat aaatcgagtc accaaaccac ttgccttttt 481 taacgagact tgttcaccaa cttgatacaa aagtcattat cctatgcaaa tcaataatca 541 tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg gataatttca caatatgtta 601 tacgataaag aagttacttt tccaagaaat tcactgattt tataagccca cttgcattag 661 ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc acgaagaatt ctagaaaata 721 cgaaatacgc ttcaatgcag tgggacccac ggttcaatta ttgccaattt tcagctccac 781 cgtatattta aaaaataaaa cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa 841 tctcaacggc tggatcttat gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat 901 aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa ctcaaagcac 961 aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt gcgtgtaaac 1021 aacgctcaat acacgtgtca ttttattatt agctattgct tcaccgcctt agctttctcg 1081 tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc caaagagctc 1141 ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct ctcaattctc 1201 tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt cgattttgtt 1261 ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat cttagatcga 1321 agacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag ggtttcatag 1381 atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct tcgatttgtg 1441 atttctatct agatctggtg ttagtttcta gtttgtgcga tcgaatttgt cgattaatct 1501 gagtttttct gattaacagg tacaagctta cgcgtcctcc ataactatgg gcaagggcga 1561 ggaactgttc actggcgtgg tcccaatcct ggtggaactg gatggtgatg tgaacgggca 1621 caagttctcc gtcagcggag agggtgaagg tgatgccacc tacggaaagc tcaccctgaa 1681 gttcatctgc actaccggaa agctccctgt tccgtggcca accctcgtca ccactttcac 1741 ctacggtgtt cagtgcttct cccggtaccc agatcacatg aagcagcatg acttcttcaa 1801 gagcgccatg cccgaaggct acgtgcaaga aaggactatc ttcttcaagg atgacgggaa 1861 ctacaagaca cgtgccgaag tcaagttcga aggtgatacc ctggtgaacc gcatcgagct 1921 gaaaggtaag tttctgcttc tacctttgat atatatataa taattatcat taattagtag 1981 taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt 2041 ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat 2101 ttgttgatgt gcaggtatcg atttcaagga agatggaaac atcctcggac acaagctgga 2161 gtacaactac aactcccaca acgtatacat catggccgac aagcagaaga acggcatcaa 2221 ggtgaacttc aagatcaggc acaacatcga agatggaagc gtgcaactgg cggaccacta 2281 ccagcagaac acgcccatcg gcgatggccc tgtcctgctg ccggacaacc attacctgtc 2341 cacgcaatct gccctctcca aggaccccaa cgagaagagg gaccacatgg tcctgctgga 2401 gttcgtgacg gctgctggga tcacgcatgg catggatgaa ctctacaagt gactcgtcga 2461 ccctgcttta atgagatatg cgagacgcct atgatcgcat gatatttgct ttcaattctg 2521 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt 2581 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc 2641 aatttactga ttgtcctcga caggccttaa gggccagatc ttgggcccgg tacccaattc 2701 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga 2761 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 2821 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 2881 atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 2941 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 3001 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 3061 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 3121 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 3181 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 3241 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 3301 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga 3361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 3421 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 3481 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 3541 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 3601 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 3661 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 3721 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 3781 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 3841 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 3901 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 3961 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 4021 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 4081 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 4141 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 4201 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 4261 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 4321 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 4381 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 4441 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 4501 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 4561 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 4621 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 4681 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 4741 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 4801 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 4861 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 4921 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 4981 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 5041 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 5101 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 5161 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 5221 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 5281 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 5341 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 5401 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 5461 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac 5521 cctcactaaa gggaacaaaa gctg //