LOCUS B593p35s-mGFPM-o 4489 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of B442pmGFPM-ocs* into B495p35s-MoGusi-ocs* ACCESSION B593p35s-mGFPM-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4489) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B495p35s-MoGusi-ocs* Fragment ends: SalI and HindIII Fragment size: 3557 Insert molecule: B442pmGFPM-ocs* Fragment ends: HindIII and SalI Fragment size: 932 GELR Sequencing primers OR2 GTGCACAACAGAATTGAAAGCAAA FEATURES Location/Qualifiers misc_feature 118..536 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" misc_signal 447..536 /label=Minimal promotor /SECDrawAs="Label" misc_feature 537..538 /product="T-start" /SECDrawAs="Region" /SECStyleId=1 CDS 564..942 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 943..1131 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 1132..1469 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1478..1673 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2489..3349 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 3392..4108 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagtggatc ccccgggtca 121 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag 181 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc 241 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca 301 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc 361 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt 421 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc 481 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac 541 aagcttacgc gtcctccacc gaaatgggca agggcgagga actgttcact ggcgtggtcc 601 caatcctggt ggaactggat ggtgatgtga acgggcacaa gttctccgtc agcggagagg 661 gtgaaggtga tgccacctac ggaaagctca ccctgaagtt catctgcact accggaaagc 721 tccctgttcc gtggccaacc ctcgtcacca ctttcaccta cggtgttcag tgcttctccc 781 ggtacccaga tcacatgaag cagcatgact tcttcaagag cgccatgccc gaaggctacg 841 tgcaagaaag gactatcttc ttcaaggatg acgggaacta caagacacgt gccgaagtca 901 agttcgaagg tgataccctg gtgaaccgca tcgagctgaa aggtaagttt ctgcttctac 961 ctttgatata tatataataa ttatcattaa ttagtagtaa tataatattt caaatatttt 1021 tttcaaaata aaagaatgta gtatatagca attgcttttc tgtagtttat aagtgtgtat 1081 attttaattt ataacttttc taatatatga ccaaaatttg ttgatgtgca ggtatcgatt 1141 tcaaggaaga tggaaacatc ctcggacaca agctggagta caactacaac tcccacaacg 1201 tatacatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atcaggcaca 1261 acatcgaaga tggaagcgtg caactggcgg accactacca gcagaacacg cccatcggcg 1321 atggccctgt cctgctgccg gacaaccatt acctgtccac gcaatctgcc ctctccaagg 1381 accccaacga gaagagggac cacatggtcc tgctggagtt cgtgacggct gctgggatca 1441 cgcatggcat ggatgaactc tacaagtgac tcgtcgaccc tgctttaatg agatatgcga 1501 gacgcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg 1561 agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc 1621 gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg tcctcgacag 1681 gccttaaggg ccagatcttg ggcccggtac ccaattcgcc ctatagtgag tcgtattaca 1741 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 1801 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 1861 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat 1921 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 1981 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 2041 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 2101 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 2161 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 2221 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 2281 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 2341 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 2401 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 2461 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 2521 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 2581 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 2641 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 2701 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 2761 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 2821 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 2881 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 2941 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 3001 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 3061 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 3121 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 3181 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 3241 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 3301 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 3361 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 3421 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 3481 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 3541 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 3601 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 3661 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 3721 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 3781 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 3841 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 3901 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 3961 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 4021 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 4081 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 4141 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 4201 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 4261 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 4321 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 4381 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 4441 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaattt //