LOCUS B472p5oM-LH 10216 bp DNA circular SYN 22-JAN-2016 DEFINITION B472p5oM-LH rotated to position 9969 ACCESSION B472p5oM-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10216) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: 269p6i-TE9* Fragment ends: MluI and XhoI Fragment size: 8426 Insert molecule: EPSPS-Xho-Mlu* Fragment ends: XhoI and MluI Fragment size: 1790 Primer for gene synthesis XhoEP cccgtcctcgagtcctccaccgaaATGGCCCAGATCAAC MluEP CCCGacgcgTTAGGCAGCCTTGGT Sequencing primers 35S GCACAATCCCACTATCCTT Maxi RBCS-TR2 CACAGTAAATTACAAGCACAAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..1485 /gene="EPSPS" /translation="MAQINNMAQGIQTLNPNSNFHKPQVPKSSSFLVFGSKKLKNSAN SMLVLKKDSIFMQKFCSFRISASVATACMLHGASSRPATARKSSGLSGTVRIPGDKSI SHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLL APEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKS EDGDRLPVTLRGPKTPTPITYRVPMASAQ" /SECDrawAs="Gene" /SECStyleId=1 /SECName="epsps" misc_feature 1486..1674 /gene="int" /product="Intron STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron STLS1" CDS 1675..2517 /gene="epsps" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2527..2817 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2993..3016 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3551..4248 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4797..8502 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8686..9696 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccac cgaaatggcc cagatcaaca acatggctca gggcattcag 781 accctcaacc ccaattccaa cttccacaag ccacaagtgc ctaagtctag ctccttcctc 841 gtcttcggct cgaagaagtt gaagaacagc gccaactcca tgcttgttct caagaaagac 901 tcaatcttca tgcagaagtt ttgcagcttc cgcatctccg caagtgtggc cactgcttgc 961 atgttgcacg gtgcctccag ccgtccggcg accgccagga agtcttccgg cctctcgggg 1021 accgtcagaa tccccggaga caagagcatt tcccaccgct cattcatgtt cggcggcctg 1081 gccagcggag agacaaggat caccggcctt ctggagggtg aggatgtcat caacaccggg 1141 aaggctatgc aggctatggg agcccgcatc cggaaggaag gcgacacgtg gattatcgac 1201 ggcgtgggaa acggcggtct cctcgcccca gaggcaccct tggacttcgg caacgccgcc 1261 accggatgcc gtctgactat ggggcttgtt ggcgtgtacg atttcgactc caccttcatc 1321 ggagacgctt ccctcaccaa gcgccctatg ggcagggtcc taaatccgct ccgtgagatg 1381 ggcgtgcaag tcaagagcga ggacggagac cgcctgccag ttaccttgcg aggccccaag 1441 actccaacac ccatcaccta cagggtgcct atggcctctg cgcaggtaag tttctgcttc 1501 tacctttgat atatatataa taattatcat taattagtag taatataata tttcaaatat 1561 ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt tataagtgtg 1621 tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt gcaggtcaag 1681 tccgccgtgc tccttgccgg gctgaacact ccgggtatta ccaccgtcat cgagcccatt 1741 atgaccagag atcataccga gaagatgctc cagggattcg gcgctaactt gacggttgaa 1801 accgacgccg acggcgtgcg caccatccgt ctggagggaa ggggcaaact caccggccaa 1861 gtcatcgacg tgccaggaga tcccagctcg actgcttttc cacttgtagc cgccctcttg 1921 gtccctggtt ccgacgtgac catcctgaac gttcttatga acccgacccg caccgggctc 1981 atcttgactc tccaggagat gggcgctgac atcgaagtca ttaaccccag gctggccgga 2041 ggcgaggacg tggcagattt gcgcgtccgt agttccaccc tgaagggtgt gacagttcca 2101 gaggaccggg ctcccagcat gatcgacgag taccctatcc ttgccgtcgc cgcggccttc 2161 gccgagggcg ctaccgtgat gaacggattg gaggagctgc gcgtcaagga gtcagacaga 2221 ctctccgccg tggccaatgg gctcaagctc aacggcgttg actgcgatga gggagaaacc 2281 agccttgtcg tgcgtggcag gccggacggc aagggactcg gtaacgcttc tggggccgca 2341 gtcgccacgc acctggacca ccgcatcgcg atgtccttcc tcgtgatggg ccttgtttcg 2401 gagaacccag tcaccgtgga cgatgccacc atgattgcta ctagcttccc cgagttcatg 2461 gacttgatgg ccggactggg agccaagatc gagctgtccg acaccaaggc tgcctaacgc 2521 gttcgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat 2581 ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag 2641 agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc 2701 ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta 2761 aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacactgaa 2821 gcctgcaggc atgcaagctg atccactaga ggccatggcg gccgcactag tggatccccc 2881 gggatgcatg aattcaagct tatcgatgtc gacaggcctt aagggccaga tcttgggccc 2941 ggtacccgat cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat 3001 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag 3061 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc 3121 tcgggagtgc ttggcattcc gtgcgataat gacttctgtt caaccaccca aacgtcggaa 3181 agcctgacga cggagcagca ttccaaaaag atcccttggc tcgtctgggt cggctagaag 3241 gtcgagtggg ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc gcagcgccga 3301 aaaatcctcg atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg 3361 gccgacgtcc tggccagtca tcacgcgcca aagttccgtc acaggatgat ctggcgcgag 3421 ttgctggatc tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa tatccgaacg 3481 cagcaagata tcgcggtgca tctcggtctt gcctgggcag tcgccgccga cgccgttgat 3541 gtggacgccg aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 3601 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 3661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 3721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 3781 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 3841 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 3901 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3961 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 4021 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 4081 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 4141 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 4201 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 4261 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 4321 gttatcccct gattctgtgg ataaccgatt accgcctttg agtgagctga taccgctcgc 4381 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 4441 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt 4501 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact 4561 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 4621 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 4681 ggttttcacc gtcatcaccg aaacgcgcga ggcaggggta cgtcgaggtc gatccaaccc 4741 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac 4801 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt 4861 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac 4921 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac 4981 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc 5041 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta 5101 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac 5161 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag 5221 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt 5281 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag 5341 gcgcgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc 5401 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg 5461 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc 5521 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc 5581 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt 5641 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc 5701 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc 5761 tcgcggcctg gccggcgagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt 5821 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag 5881 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg 5941 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc 6001 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg 6061 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa 6121 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc 6181 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga 6241 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg 6301 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga 6361 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg 6421 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg 6481 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt 6541 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc 6601 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg 6661 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa 6721 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc 6781 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga 6841 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg 6901 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga 6961 atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat 7021 gacctggtgg agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa 7081 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa 7141 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat 7201 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg 7261 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt 7321 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggcgagtgt gtgggattac 7381 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg 7441 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc 7501 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac 7561 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta 7621 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg 7681 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac 7741 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt 7801 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg 7861 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag 7921 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc 7981 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa 8041 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc 8101 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg 8161 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata 8221 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa 8281 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg 8341 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc 8401 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc 8461 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccggtgggta tgcctgacga 8521 tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg 8581 ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag 8641 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac 8701 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct 8761 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt 8821 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt 8881 cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa 8941 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat 9001 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt 9061 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa 9121 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg 9181 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg 9241 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct 9301 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa 9361 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta 9421 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc 9481 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca 9541 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt 9601 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac 9661 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga 9721 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac 9781 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac 9841 acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga 9901 agtaatcgca acatagcttg cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc 9961 gttcaaatac tttgcgatcg tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat 10021 cgactgcttc tctcgcaacg ccatccgacg gatgatgttt aaaagtccca tgtggatcac 10081 tccgttgccc cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg 10141 aaattatctg cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt 10201 gcaaagcggc agcggc //