LOCUS B473p6oM-LH 9666 bp DNA circular SYN 22-JAN-2016 DEFINITION B473p6oM-LH rotated to position 9419 ACCESSION B473p6oM-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9666) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B474p7oM-LH* Fragment ends: BspEI and XhoI Fragment size: 8433 Insert molecule: hpt-xho-Bspe* Fragment ends: XhoI and BspEI Fragment size: 1233 Primer for gene synthesis XhoHpt cccgtcctcgagtcctccaccgaaATGAAGAAACCGGAGC BspeHpt CCCGTCtccggaTCACTCCTTGGCC Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC Maxi only 35S GCACAATCCCACTATCCTT RBCS-TR2 CACAGTAAATTACAAGCACAAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..1314 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 1315..1503 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 1504..1959 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1977..2267 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2443..2466 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3001..3698 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4247..7952 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8136..9146 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccac cgaaatgaag aaaccggagc tgaccgccac ttccgtcgaa 781 aagttcctca tcgagaagtt tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa 841 gagtcccgcg cgttcagctt tgatgttggc gggcgcgggt acgtcctccg cgtgaactct 901 tgcgctgacg gcttctataa agatcggtac gtttacaggc atttcgcctc ggcggccctg 961 cctattcccg aagtcctcga catcggcgag ttttccgaga gccttacgta ttgtatttcc 1021 cgccgggctc aaggggtgac cctccaggat ctgccggaaa ctgagttgcc tgcggttctc 1081 cagcccgtcg ccgaggctat ggacgccatc gccgcggcgg atctttctca aacgtcgggc 1141 ttcgggccgt tcggccctca ggggattggc cagtacacca cttggaggga ctttatctgc 1201 gctatcgccg atccgcacgt gtatcattgg caaaccgtta tggacgatac ggtctccgcg 1261 agcgtggccc aggctctgga cgaactcatg ttgtgggccg aggattgccc cgaggtaagt 1321 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat 1381 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt 1441 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg 1501 caggtccgcc acctggttca tgcggacttc ggctctaata acgtgctcac cgataatggg 1561 cggattactg ccgtcatcga ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg 1621 gcgaacattt tcttctggag gccttggctt gcctgcatgg aacagcaaac gcgctacttt 1681 gagcggaggc acccggagct ggcggggtct ccccgcctca gggcctatat gttgcggatc 1741 ggcctcgacc agctttacca gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg 1801 caaggccgct gtgacgctat tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc 1861 gcccggcggt cggctgccgt ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc 1921 aacaggcggc cttccacccg cccgcgggcc aaggagtgat ccggaatcgc gttcgagtat 1981 tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt 2041 ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa 2101 tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg 2161 tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc 2221 aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacactgaa gcctgcaggc 2281 atgcaagctg atccactaga ggccatggcg gccgcactag tggatccccc gggatgcatg 2341 aattcaagct tatcgatgtc gacaggcctt aagggccaga tcttgggccc ggtacccgat 2401 cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat atattggcgg 2461 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa 2521 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggagtgc 2581 ttggcattcc gtgcgataat gacttctgtt caaccaccca aacgtcggaa agcctgacga 2641 cggagcagca ttccaaaaag atcccttggc tcgtctgggt cggctagaag gtcgagtggg 2701 ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc gcagcgccga aaaatcctcg 2761 atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg gccgacgtcc 2821 tggccagtca tcacgcgcca aagttccgtc acaggatgat ctggcgcgag ttgctggatc 2881 tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa tatccgaacg cagcaagata 2941 tcgcggtgca tctcggtctt gcctgggcag tcgccgccga cgccgttgat gtggacgccg 3001 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 3061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 3121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 3181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 3241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 3301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 3361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 3421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 3481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 3541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 3601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 3661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 3721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 3781 gattctgtgg ataaccgatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 3841 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc 3901 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct 3961 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc 4021 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 4081 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 4141 gtcatcaccg aaacgcgcga ggcaggggta cgtcgaggtc gatccaaccc ctccgctgct 4201 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca 4261 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt 4321 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca 4381 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga 4441 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca 4501 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg 4561 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca 4621 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg 4681 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg 4741 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcgcgaggcg 4801 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga 4861 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga 4921 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg 4981 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac 5041 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac 5101 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt 5161 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tcgcggcctg 5221 gccggcgagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt 5281 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca 5341 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc 5401 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg 5461 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa 5521 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc 5581 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg 5641 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc 5701 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa 5761 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag 5821 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac 5881 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc 5941 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta 6001 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca 6061 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc 6121 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca 6181 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa 6241 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc 6301 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc 6361 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga 6421 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg 6481 agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg 6541 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag 6601 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc 6661 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc 6721 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc 6781 acgtagaggt ttccgcaggg ccggccggca tggcgagtgt gtgggattac gacctggtac 6841 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca 6901 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg 6961 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg 7021 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg 7081 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg 7141 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc 7201 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc 7261 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac 7321 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt 7381 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag 7441 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc 7501 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg 7561 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga 7621 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa 7681 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg 7741 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc 7801 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct 7861 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc 7921 cactcgaccg ccggcgccca catcaaggca ccggtgggta tgcctgacga tgcgtggaga 7981 ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca 8041 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag 8101 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa 8161 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact 8221 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg 8281 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa 8341 caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg 8401 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct 8461 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg 8521 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt 8581 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca 8641 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg 8701 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga 8761 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc 8821 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc 8881 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca 8941 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc 9001 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag 9061 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg 9121 agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg 9181 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta 9241 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg 9301 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca 9361 acatagcttg cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc gttcaaatac 9421 tttgcgatcg tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat cgactgcttc 9481 tctcgcaacg ccatccgacg gatgatgttt aaaagtccca tgtggatcac tccgttgccc 9541 cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg aaattatctg 9601 cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt gcaaagcggc 9661 agcggc //