LOCUS B481p4o2x35s-LH 11024 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of 35s-Xba-xho* into B471p4oM-LH* ACCESSION B481p4o2x35s-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11024) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B471p4oM-LH* Fragment ends: XhoI and XbaI Fragment size: 10229 Insert molecule: 35s-Xba-xho* Fragment ends: XbaI and XhoI Fragment size: 795 Primer for gene synthesis 35sXba CCCGTCtctagaCGGGCCAACATGGT 35sXho GGGGCCCTCGAGAGAGATAGA Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 314..1098 /gene="P2x35s" /product="Promoter" /SECDrawAs="Region" /SECStyleId=1 CDS 1116..1754 /gene="ahas" /translation="MAAATTTTTTSSSISFSTKPSPSSSKSPLPISRFSLPFSLNPNK SSSSSRRRGIKSSSPSSISAVLNTTTNVTTTPSPTKPTKPETFISRFAPDQPRKGADI LVEALERQGVETVFAYPGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGK PGICIATSGPGATNLVSGLADALLDSVPLVAITGQVPRRMIGTDAFQETPIVE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="ahas" CDS 1755..1943 /gene="int" /product="intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 1944..3317 /gene="ahas" /translation="VTRSITKHNYLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKD IQQQLAIPNWEQAMRLPGYMSRMPKPPEDSHLEQIVRLISESKKPVLYVGGGCLNSSD ELGRFVELTGIPVASTLMGLGSYPCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVR FDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAE ELKLDFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGVGQHQ MWAAQFYNYKKPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQE LATIRVENLPVKVLLLNNQHLGMVMQWEDRFYKANRAHTFLGDPAQEDEIFPNMLLFA AACGIPAARVTKKADLREAIQTMLDTPGPYLLDVICPHQEHVLPMIPSGGTFNDVITE GDGRIKY" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 3335..3625 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3801..3824 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4359..5056 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5605..9310 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9494..10504 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacg ggccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag 361 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa 421 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg 481 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct 541 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag 601 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact 661 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact 721 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac 781 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa 841 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc 901 acgaggagca tcgtggaaaa agaagacctt ccaaccacgt cttcaaagca agtggattga 961 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct 1021 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc 1081 tctacaaatc tatctctctc gagtcctcca ccgaaatggc cgctgccacc accactacca 1141 ccacctccag ctctatctcc ttctcgacca agcccagccc atcctcaagc aagtcccctc 1201 tcccgatttc ccgcttcagc ctgcccttct ctttgaaccc aaacaagtcc agctcgtcca 1261 gcaggcgtcg cggcatcaag tcctctagcc cctcctcaat ctccgcagtg cttaacacta 1321 ccaccaatgt caccaccact ccttcgccga ccaagccaac caagcccgag accttcatca 1381 gcagatttgc tcccgaccag ccaaggaagg gcgccgacat tctcgttgag gccctggagc 1441 gccaaggcgt ggaaaccgtc ttcgcctacc ctggtggggc gtctatggag atccaccagg 1501 ccctcactcg ttcctccagc atccgcaacg tgttgccgag gcacgagcag ggcggagtct 1561 tcgctgccga gggctacgcc cgctccagcg gaaaacccgg catctgcatt gctacctcgg 1621 gtccaggcgc caccaacctt gtttcaggac tcgcagatgc cctgctcgac tccgtgccct 1681 tggtcgctat caccggccaa gttcctcgta gaatgatcgg gaccgacgcc ttccaagaga 1741 ccccgatcgt cgaggtaagt ttctgcttct acctttgata tatatataat aattatcatt 1801 aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag 1861 caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat 1921 gaccaaaatt tgttgatgtg caggttactc gcagcattac caagcacaac taccttgtga 1981 tggacgtgga agacatccca aggatcatcg aggaggcctt cttcctggcg acctctggcc 2041 gtcccggacc tgtcctcgtc gatgtgccca aggacattca gcagcaactc gccatcccaa 2101 actgggagca ggctatgcgc ctgccgggct acatgtccag gatgcccaag ccacctgagg 2161 actcccattt ggagcagatc gtgcgcctta tcagcgaatc caagaagccc gtcctctacg 2221 tgggaggtgg ctgcctgaac agctcggacg agcttgggag attcgtcgag ttgaccggca 2281 ttccggtggc ctccaccctc atgggactcg gctcttaccc atgcgacgat gagctgagcc 2341 tccacatgtt gggtatgcac ggcactgttt acgccaatta tgctgtcgag cactccgacc 2401 tgcttctcgc cttcggagtg cgttttgacg accgcgtcac cggcaagctg gaggcattcg 2461 cctcaagggc taagatcgtg cacatcgaca ttgattccgc cgaaatcgga aagaacaaga 2521 cccctcacgt cagcgtttgc ggcgacgtga agctcgcctt gcaggggatg aacaaagttc 2581 ttgagaaccg cgcggaggag ctgaagctcg acttcggcgt gtggcgtaac gagcttaacg 2641 tccaaaagca gaagttcccc ttgtccttca agaccttcgg agaggccatc ccgccccagt 2701 acgctatcaa agtgctcgac gaactgaccg atggtaaggc cattatcagc actggcgtcg 2761 gacagcacca aatgtgggcc gctcagttct acaattacaa gaagccacgc cagtggctct 2821 cgtccggcgg gctcggcgca atgggattcg gcctgccagc cgccatcggt gcttctgtcg 2881 ccaacccaga cgccatcgtg gtggacattg acggcgacgg cagcttcatt atgaacgtcc 2941 aagagttggc taccatcagg gttgagaacc ttcctgtcaa agttctcctg ctcaacaacc 3001 agcatttggg catggtcatg cagtgggagg atagatttta caaggccaat cgcgcgcaca 3061 ccttccttgg ggacccggcc caggaggacg agattttccc caacatgctc ctgttcgccg 3121 ctgcctgcgg catcccagca gcccgtgtga ccaagaaggc tgacctcagg gaagccatcc 3181 aaaccatgtt ggacactccc ggaccttacc ttctggatgt gatctgcccg caccaggagc 3241 acgtcctccc aatgatcccc tccggtggca ccttcaacga cgtgattacc gagggagacg 3301 gacgcatcaa gtactaatcc ggaatcgcgt tcgagtatta tggcattggg aaaactgttt 3361 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg 3421 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt 3481 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa 3541 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg 3601 aagttaatat gaggagtaaa acactgaagc ctgcaggcat gcaagctgat ccactagagg 3661 ccatggcggc cgcactagtg gatcccccgg gatgcatgaa ttcaagctta tcgatgtcga 3721 caggccttaa gggccagatc ttgggcccgg tacccgatca gattgtcgtt tcccgccttc 3781 ggtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg 3841 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg 3901 tatgtgcatg ccaaccacag ggttcccctc gggagtgctt ggcattccgt gcgataatga 3961 cttctgttca accacccaaa cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat 4021 cccttggctc gtctgggtcg gctagaaggt cgagtgggct gctgtggctt gatccctcaa 4081 cgcggtcgcg gacgtagcgc agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga 4141 aaagcgtgat ctgcttgtcg ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa 4201 gttccgtcac aggatgatct ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg 4261 gcgggaactc cacgaaaata tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc 4321 ctgggcagtc gccgccgacg ccgttgatgt ggacgccgaa aaggatctag gtgaagatcc 4381 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 4441 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 4501 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 4561 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 4621 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 4681 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 4741 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 4801 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 4861 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 4921 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 4981 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 5041 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 5101 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgattac 5161 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 5221 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 5281 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 5341 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca 5401 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 5461 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg 5521 caggggtacg tcgaggtcga tccaacccct ccgctgctat agtgcagtcg gcttctgacg 5581 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc 5641 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa 5701 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct 5761 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca 5821 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc 5881 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct 5941 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc 6001 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg 6061 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg 6121 cacccggagc gggcgcgagg ccgccaaggc gcgaggcgtg aagtttggcc cccgccctac 6181 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt 6241 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg 6301 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt 6361 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa 6421 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg 6481 atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa 6541 atcctggccg gtttgtctga tgccaagctc gcggcctggc cggcgagctt ggccgctgaa 6601 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat 6661 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga 6721 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc 6781 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg 6841 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg 6901 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg 6961 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc 7021 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg 7081 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg 7141 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc 7201 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca 7261 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg 7321 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac 7381 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag 7441 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac 7501 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata 7561 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg 7621 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca 7681 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca 7741 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc 7801 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg cggcgcaggc 7861 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc 7921 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa 7981 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac 8041 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg 8101 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc 8161 ggccggcatg gcgagtgtgt gggattacga cctggtactg atggcggttt cccatctaac 8221 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc 8281 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga 8341 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa 8401 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa 8461 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat 8521 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt 8581 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa 8641 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt 8701 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga 8761 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat 8821 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct 8881 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc 8941 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa 9001 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa 9061 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca 9121 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc 9181 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc 9241 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca 9301 tcaaggcacc ggtgggtatg cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc 9361 cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc 9421 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta 9481 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg 9541 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat 9601 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag 9661 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga 9721 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca 9781 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa 9841 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg 9901 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct 9961 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc 10021 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga 10081 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt 10141 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt 10201 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga 10261 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc 10321 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt 10381 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc 10441 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa 10501 ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt 10561 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt 10621 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc 10681 tttttcttgt tatcgcaata gttggcgaag taatcgcaac atagcttgct tggtcgttcc 10741 gcgtgaacgt cggctcgatt gtacctgcgt tcaaatactt tgcgatcgtg ttgcgcgcct 10801 gcccggtgcg tcggctgatc tcacggatcg actgcttctc tcgcaacgcc atccgacgga 10861 tgatgtttaa aagtcccatg tggatcactc cgttgccccg tcgctcaccg tgttgggggg 10921 aaggtgcaca tggctcagtt ctcaatggaa attatctgcc taaccggctc agttctgcgt 10981 agaaaccaac atgcaagctc caccgggtgc aaagcggcag cggc //