LOCUS B516p9o2x35s-Ubi 11647 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of 594pUbi-BA* into B485p9o2x35s-LH* ACCESSION B516p9o2x35s-Ubi KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11647) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B485p9o2x35s-LH* Fragment ends: SfiI Fragment size: 9751 Insert molecule: 594pUbi-BA* Fragment ends: SfiI Fragment size: 1896 Sequencing primers E9F GTGTCAAATCGTGGCCTCTAA SphR GGGTGGTTGAACAGAAGTCAT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 314..1098 /gene="P2x35s" /SECDrawAs="Region" /SECStyleId=1 CDS 1116..1414 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 CDS 1415..1601 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 CDS 1602..2108 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2126..2416 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2480..3987 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3466..3985 /gene="int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4048..4301 /gene="Tnos" /product="NOS-T" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4424..4447 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4982..5679 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 6228..9933 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 10117..11127 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacg ggccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag 361 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa 421 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg 481 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct 541 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag 601 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact 661 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact 721 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac 781 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa 841 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc 901 acgaggagca tcgtggaaaa agaagacctt ccaaccacgt cttcaaagca agtggattga 961 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct 1021 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc 1081 tctacaaatc tatctctctc gagtcctcca ccgaaatggg agcatggatt gagcaggatg 1141 gcctccatgc tggtagcccc gctgcttggg tggagcgcct ctttggctac gactgggccc 1201 aacagaccat tgggtgcagt gatgccgctg tcttccgttt gagcgcccag ggcaggccag 1261 ttctcttcgt gaagactgac ctctccggtg ccctcaacga gttgcaggac gaagctgccc 1321 gcctctcatg gcttgccacc accggagtcc cttgcgctgc cgtgttggac gttgtcacag 1381 aggccggaag agactggctg ctcttgggcg aggtaagttt ctgcttctac ctttgatata 1441 tatataataa ttatcattaa ttagtagtaa tataatattt caaatatttt tttcaaaata 1501 aaagaatgta gtatatagca attgcttttc tgtagtttat aagtgtgtat attttaattt 1561 ataacttttc taatatatga ccaaaatttg ttgatgtgca ggtgccaggc caagacctcc 1621 tgagctccca cctggcccca gccgagaagg tctccatcat ggcagacgcc atgcgtcgcc 1681 tccacaccct ggacccagcc acctgcccct tcgaccacca ggccaagcac aggatcgaac 1741 gtgcacgcac taggatggag gccggccttg tggaccagga tgaccttgac gaggagcacc 1801 agggccttgc tcccgccgag ctgttcgccc gcctcaaggc tagaatgcct gacggagagg 1861 acttggtggt cacccacggg gatgcctgcc tccccaacat catggttgag aacggacgct 1921 tctctggctt catcgactgc ggtcgtctgg gcgtggccga ccgctaccaa gacatcgccc 1981 tggccaccag ggacatcgct gaggaacttg gaggggagtg ggccgatcgt ttcttggtcc 2041 tctacggcat tgctgccccc gactcccaga ggatcgcctt ctaccgcctg cttgacgagt 2101 tcttctgatc cggaatcgcg ttcgagtatt atggcattgg gaaaactgtt tttcttgtac 2161 catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa 2221 atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta 2281 atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc 2341 aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata 2401 tgaggagtaa aacactgaag cctgcaggca tgcaagctga tccactagag gccatggggg 2461 ccaatttaaa tggcgtacgt gcagtgcagc gtgacccggt cgtgcccctc tctagagata 2521 atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt 2581 tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg aataatataa 2641 tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat 2701 ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc tttttagtgt 2761 gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc atccatttta 2821 ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca 2881 tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt 2941 tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt aaacaaatac 3001 cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag ataatgccag 3061 cctgttaaac gccgtcgatc gacgagtcta acggacacca accagcgaac cagcagcgtc 3121 gcgtcgggcc aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc 3181 gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg 3241 agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc 3301 tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat 3361 agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac 3421 aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc 3481 tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg 3541 gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct 3601 agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt 3661 gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatctagg 3721 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat 3781 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa 3841 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt 3901 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc 3961 ctgttgtttg gtgttacttc tgcaggtact agtggatccc ccgggctgca ggaattcaag 4021 cttacgcgtg tcgactcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta 4081 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt 4141 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt 4201 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag 4261 gataaattat cgcgcgcggt gtcatctatg ttactagatc gctcgacgca tgctggtacc 4321 tgacgtctgc ggccgcatta attaaggccc taagggccag atcttgggcc cggtacccga 4381 tcagattgtc gtttcccgcc ttcggtttaa actatcagtg tttgacagga tatattggcg 4441 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa 4501 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtg 4561 cttggcattc cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg 4621 acggagcagc attccaaaaa gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg 4681 gctgctgtgg cttgatccct caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc 4741 gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc 4801 ctggccagtc atcacgcgcc aaagttccgt cacaggatga tctggcgcga gttgctggat 4861 ctcgccttca atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat 4921 atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc 4981 gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 5041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 5101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 5161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 5221 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 5281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 5341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 5401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 5461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 5521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 5581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 5641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 5701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 5761 tgattctgtg gataaccgat taccgccttt gagtgagctg ataccgctcg ccgcagccga 5821 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt 5881 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc 5941 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg 6001 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 6061 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 6121 cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc cctccgctgc 6181 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac 6241 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg 6301 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac 6361 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg 6421 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc 6481 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc 6541 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac 6601 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc 6661 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc 6721 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcgcgaggc 6781 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg 6841 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg 6901 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc 6961 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa 7021 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta 7081 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg 7141 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct 7201 ggccggcgag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat 7261 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac 7321 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg 7381 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct 7441 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca 7501 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg 7561 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc 7621 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc 7681 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca 7741 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga 7801 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta 7861 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc 7921 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt 7981 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc 8041 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt 8101 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc 8161 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata 8221 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg 8281 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg 8341 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg 8401 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg 8461 gagaagttga aggcggcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc 8521 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca 8581 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt 8641 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc 8701 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg 8761 cacgtagagg tttccgcagg gccggccggc atggcgagtg tgtgggatta cgacctggta 8821 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac 8881 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc 8941 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac 9001 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt 9061 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc 9121 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg 9181 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc 9241 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa 9301 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg 9361 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta 9421 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag 9481 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg 9541 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg 9601 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa 9661 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg 9721 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt 9781 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc 9841 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg 9901 ccactcgacc gccggcgccc acatcaaggc accggtgggt atgcctgacg atgcgtggag 9961 accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc 10021 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa 10081 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga 10141 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac 10201 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg 10261 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa 10321 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag 10381 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc 10441 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc 10501 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct 10561 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac 10621 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat 10681 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg 10741 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat 10801 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg 10861 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac 10921 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc 10981 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa 11041 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc 11101 gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc 11161 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact 11221 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg 11281 gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc 11341 aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata 11401 ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga tcgactgctt 11461 ctctcgcaac gccatccgac ggatgatgtt taaaagtccc atgtggatca ctccgttgcc 11521 ccgtcgctca ccgtgttggg gggaaggtgc acatggctca gttctcaatg gaaattatct 11581 gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg 11641 cagcggc //