LOCUS B482p5o2x35s-LH 10587 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of 35s-Xba-xho* into B472p5oM-LH* ACCESSION B482p5o2x35s-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10587) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B472p5oM-LH* Fragment ends: XhoI and XbaI Fragment size: 9792 Insert molecule: 35s-Xba-xho* Fragment ends: XbaI and XhoI Fragment size: 795 Primer for gene synthesis 35sXba CCCGTCtctagaCGGGCCAACATGGT 35sXho GGGGCCCTCGAGAGAGATAGA Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 314..1098 /gene="P2x35s" /SECDrawAs="Region" /SECStyleId=1 CDS 1116..1856 /gene="EPSPS" /translation="MAQINNMAQGIQTLNPNSNFHKPQVPKSSSFLVFGSKKLKNSAN SMLVLKKDSIFMQKFCSFRISASVATACMLHGASSRPATARKSSGLSGTVRIPGDKSI SHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLL APEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKS EDGDRLPVTLRGPKTPTPITYRVPMASAQ" /SECDrawAs="Gene" /SECStyleId=1 /SECName="epsps" misc_feature 1857..2045 /gene="int" /product="Intron STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron STLS1" CDS 2046..2888 /gene="epsps" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2898..3188 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3364..3387 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3922..4619 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5168..8873 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9057..10067 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacg ggccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag 361 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa 421 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg 481 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct 541 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag 601 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact 661 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact 721 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac 781 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa 841 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc 901 acgaggagca tcgtggaaaa agaagacctt ccaaccacgt cttcaaagca agtggattga 961 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct 1021 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc 1081 tctacaaatc tatctctctc gagtcctcca ccgaaatggc ccagatcaac aacatggctc 1141 agggcattca gaccctcaac cccaattcca acttccacaa gccacaagtg cctaagtcta 1201 gctccttcct cgtcttcggc tcgaagaagt tgaagaacag cgccaactcc atgcttgttc 1261 tcaagaaaga ctcaatcttc atgcagaagt tttgcagctt ccgcatctcc gcaagtgtgg 1321 ccactgcttg catgttgcac ggtgcctcca gccgtccggc gaccgccagg aagtcttccg 1381 gcctctcggg gaccgtcaga atccccggag acaagagcat ttcccaccgc tcattcatgt 1441 tcggcggcct ggccagcgga gagacaagga tcaccggcct tctggagggt gaggatgtca 1501 tcaacaccgg gaaggctatg caggctatgg gagcccgcat ccggaaggaa ggcgacacgt 1561 ggattatcga cggcgtggga aacggcggtc tcctcgcccc agaggcaccc ttggacttcg 1621 gcaacgccgc caccggatgc cgtctgacta tggggcttgt tggcgtgtac gatttcgact 1681 ccaccttcat cggagacgct tccctcacca agcgccctat gggcagggtc ctaaatccgc 1741 tccgtgagat gggcgtgcaa gtcaagagcg aggacggaga ccgcctgcca gttaccttgc 1801 gaggccccaa gactccaaca cccatcacct acagggtgcc tatggcctct gcgcaggtaa 1861 gtttctgctt ctacctttga tatatatata ataattatca ttaattagta gtaatataat 1921 atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct tttctgtagt 1981 ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa tttgttgatg 2041 tgcaggtcaa gtccgccgtg ctccttgccg ggctgaacac tccgggtatt accaccgtca 2101 tcgagcccat tatgaccaga gatcataccg agaagatgct ccagggattc ggcgctaact 2161 tgacggttga aaccgacgcc gacggcgtgc gcaccatccg tctggaggga aggggcaaac 2221 tcaccggcca agtcatcgac gtgccaggag atcccagctc gactgctttt ccacttgtag 2281 ccgccctctt ggtccctggt tccgacgtga ccatcctgaa cgttcttatg aacccgaccc 2341 gcaccgggct catcttgact ctccaggaga tgggcgctga catcgaagtc attaacccca 2401 ggctggccgg aggcgaggac gtggcagatt tgcgcgtccg tagttccacc ctgaagggtg 2461 tgacagttcc agaggaccgg gctcccagca tgatcgacga gtaccctatc cttgccgtcg 2521 ccgcggcctt cgccgagggc gctaccgtga tgaacggatt ggaggagctg cgcgtcaagg 2581 agtcagacag actctccgcc gtggccaatg ggctcaagct caacggcgtt gactgcgatg 2641 agggagaaac cagccttgtc gtgcgtggca ggccggacgg caagggactc ggtaacgctt 2701 ctggggccgc agtcgccacg cacctggacc accgcatcgc gatgtccttc ctcgtgatgg 2761 gccttgtttc ggagaaccca gtcaccgtgg acgatgccac catgattgct actagcttcc 2821 ccgagttcat ggacttgatg gccggactgg gagccaagat cgagctgtcc gacaccaagg 2881 ctgcctaacg cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt 2941 gtgcttgtaa tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat 3001 ggatggagaa gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt 3061 tgttttttct cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt 3121 tgttttgagt aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt 3181 aaaacactga agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta 3241 gtggatcccc cgggatgcat gaattcaagc ttatcgatgt cgacaggcct taagggccag 3301 atcttgggcc cggtacccga tcagattgtc gtttcccgcc ttcggtttaa actatcagtg 3361 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg 3421 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca 3481 cagggttccc ctcgggagtg cttggcattc cgtgcgataa tgacttctgt tcaaccaccc 3541 aaacgtcgga aagcctgacg acggagcagc attccaaaaa gatcccttgg ctcgtctggg 3601 tcggctagaa ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc gcggacgtag 3661 cgcagcgccg aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg 3721 tcgctctttc ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt cacaggatga 3781 tctggcgcga gttgctggat ctcgccttca atccgggtct gtggcgggaa ctccacgaaa 3841 atatccgaac gcagcaagat atcgcggtgc atctcggtct tgcctgggca gtcgccgccg 3901 acgccgttga tgtggacgcc gaaaaggatc taggtgaaga tcctttttga taatctcatg 3961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 4021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 4081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 4141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 4201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 4261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 4321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 4381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 4441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 4501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 4561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 4621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 4681 ttctttcctg cgttatcccc tgattctgtg gataaccgat taccgccttt gagtgagctg 4741 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 4801 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt 4861 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc 4921 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg 4981 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 5041 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt 5101 cgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt 5161 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt 5221 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc 5281 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca 5341 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca 5401 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc 5461 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca 5521 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta 5581 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt 5641 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg 5701 aggccgccaa ggcgcgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga 5761 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac 5821 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc 5881 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc 5941 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca 6001 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt 6061 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc 6121 tgatgccaag ctcgcggcct ggccggcgag cttggccgct gaagaaaccg agcgccgccg 6181 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat 6241 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt 6301 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa 6361 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg 6421 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac 6481 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg 6541 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca 6601 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag 6661 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc 6721 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt 6781 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca 6841 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa 6901 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg 6961 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag 7021 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg 7081 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag 7141 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg 7201 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc 7261 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc 7321 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg 7381 cgctgggtga tgacctggtg gagaagttga aggcggcgca ggccgcccag cggcaacgca 7441 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag 7501 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg 7561 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca 7621 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc 7681 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggcgagtg 7741 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat 7801 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac 7861 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca 7921 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc 7981 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa 8041 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag 8101 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca 8161 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt 8221 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca 8281 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg 8341 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg 8401 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc 8461 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga 8521 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag 8581 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta 8641 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc 8701 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc 8761 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc 8821 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accggtgggt 8881 atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg 8941 cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg 9001 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc 9061 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg 9121 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc 9181 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc 9241 agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag 9301 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc 9361 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg 9421 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg 9481 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca 9541 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat 9601 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca 9661 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag 9721 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa 9781 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta 9841 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg 9901 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac 9961 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat 10021 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc 10081 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca 10141 taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata 10201 ataagcccta cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca 10261 atagttggcg aagtaatcgc aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg 10321 attgtacctg cgttcaaata ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg 10381 atctcacgga tcgactgctt ctctcgcaac gccatccgac ggatgatgtt taaaagtccc 10441 atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc acatggctca 10501 gttctcaatg gaaattatct gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag 10561 ctccaccggg tgcaaagcgg cagcggc //