LOCUS B483p6o2x35s-LH 10037 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of 35s-Xba-xho* into B473p6oM-LH* ACCESSION B483p6o2x35s-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10037) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B473p6oM-LH* Fragment ends: XhoI and XbaI Fragment size: 9242 Insert molecule: 35s-Xba-xho* Fragment ends: XbaI and XhoI Fragment size: 795 Primer for gene synthesis 35sXba CCCGTCtctagaCGGGCCAACATGGT 35sXho GGGGCCCTCGAGAGAGATAGA Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 314..1098 /gene="P2x35s" /SECDrawAs="Region" /SECStyleId=1 CDS 1116..1685 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 1686..1874 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 1875..2330 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2348..2638 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2814..2837 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3372..4069 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4618..8323 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8507..9517 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacg ggccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag 361 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa 421 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg 481 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct 541 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag 601 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact 661 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact 721 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac 781 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa 841 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc 901 acgaggagca tcgtggaaaa agaagacctt ccaaccacgt cttcaaagca agtggattga 961 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct 1021 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc 1081 tctacaaatc tatctctctc gagtcctcca ccgaaatgaa gaaaccggag ctgaccgcca 1141 cttccgtcga aaagttcctc atcgagaagt ttgatagcgt gtctgacctt atgcagttgt 1201 cggagggcga agagtcccgc gcgttcagct ttgatgttgg cgggcgcggg tacgtcctcc 1261 gcgtgaactc ttgcgctgac ggcttctata aagatcggta cgtttacagg catttcgcct 1321 cggcggccct gcctattccc gaagtcctcg acatcggcga gttttccgag agccttacgt 1381 attgtatttc ccgccgggct caaggggtga ccctccagga tctgccggaa actgagttgc 1441 ctgcggttct ccagcccgtc gccgaggcta tggacgccat cgccgcggcg gatctttctc 1501 aaacgtcggg cttcgggccg ttcggccctc aggggattgg ccagtacacc acttggaggg 1561 actttatctg cgctatcgcc gatccgcacg tgtatcattg gcaaaccgtt atggacgata 1621 cggtctccgc gagcgtggcc caggctctgg acgaactcat gttgtgggcc gaggattgcc 1681 ccgaggtaag tttctgcttc tacctttgat atatatataa taattatcat taattagtag 1741 taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt 1801 ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat 1861 ttgttgatgt gcaggtccgc cacctggttc atgcggactt cggctctaat aacgtgctca 1921 ccgataatgg gcggattact gccgtcatcg actggtccga agctatgttt ggcgattcgc 1981 agtacgaagt ggcgaacatt ttcttctgga ggccttggct tgcctgcatg gaacagcaaa 2041 cgcgctactt tgagcggagg cacccggagc tggcggggtc tccccgcctc agggcctata 2101 tgttgcggat cggcctcgac cagctttacc agtccctggt tgatgggaat ttcgacgatg 2161 ctgcctgggc gcaaggccgc tgtgacgcta ttgtcaggag cggcgcgggg accgtgggcc 2221 gcactcagat cgcccggcgg tcggctgccg tttggacgga tgggtgcgtc gaagtgctcg 2281 cggacagcgg caacaggcgg ccttccaccc gcccgcgggc caaggagtga tccggaatcg 2341 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa 2401 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa 2461 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct 2521 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt 2581 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga 2641 agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta gtggatcccc 2701 cgggatgcat gaattcaagc ttatcgatgt cgacaggcct taagggccag atcttgggcc 2761 cggtacccga tcagattgtc gtttcccgcc ttcggtttaa actatcagtg tttgacagga 2821 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa 2881 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc 2941 ctcgggagtg cttggcattc cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga 3001 aagcctgacg acggagcagc attccaaaaa gatcccttgg ctcgtctggg tcggctagaa 3061 ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc gcggacgtag cgcagcgccg 3121 aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc 3181 ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt cacaggatga tctggcgcga 3241 gttgctggat ctcgccttca atccgggtct gtggcgggaa ctccacgaaa atatccgaac 3301 gcagcaagat atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga 3361 tgtggacgcc gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 3421 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 3481 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 3541 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 3601 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 3661 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 3721 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 3781 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 3841 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 3901 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 3961 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 4021 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 4081 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 4141 cgttatcccc tgattctgtg gataaccgat taccgccttt gagtgagctg ataccgctcg 4201 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat 4261 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag 4321 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac 4381 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 4441 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 4501 aggttttcac cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc 4561 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga 4621 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt 4681 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta 4741 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga 4801 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc 4861 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct 4921 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga 4981 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga 5041 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat 5101 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa 5161 ggcgcgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc 5221 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt 5281 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc 5341 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc 5401 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg 5461 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg 5521 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag 5581 ctcgcggcct ggccggcgag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg 5641 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga 5701 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag 5761 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg 5821 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc 5881 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga 5941 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg 6001 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg 6061 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg 6121 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg 6181 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc 6241 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg 6301 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag 6361 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag 6421 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg 6481 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca 6541 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag 6601 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag 6661 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag 6721 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg 6781 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga 6841 tgacctggtg gagaagttga aggcggcgca ggccgcccag cggcaacgca tcgaggcaga 6901 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca 6961 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga 7021 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt 7081 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct 7141 tccagacggg cacgtagagg tttccgcagg gccggccggc atggcgagtg tgtgggatta 7201 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg 7261 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg 7321 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa 7381 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt 7441 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc 7501 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa 7561 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt 7621 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac 7681 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa 7741 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg 7801 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta 7861 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct 7921 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc 7981 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat 8041 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa 8101 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc 8161 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc 8221 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc 8281 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accggtgggt atgcctgacg 8341 atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc 8401 gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca 8461 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa 8521 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc 8581 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg 8641 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag 8701 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca 8761 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca 8821 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt 8881 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga 8941 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt 9001 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg 9061 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc 9121 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga 9181 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct 9241 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg 9301 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc 9361 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct 9421 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta 9481 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg 9541 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca 9601 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta 9661 cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg 9721 aagtaatcgc aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg 9781 cgttcaaata ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga 9841 tcgactgctt ctctcgcaac gccatccgac ggatgatgtt taaaagtccc atgtggatca 9901 ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc acatggctca gttctcaatg 9961 gaaattatct gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag ctccaccggg 10021 tgcaaagcgg cagcggc //