LOCUS B484p7o2x35s-LH 9565 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of 35s-Xba-xho* into B474p7oM-LH* ACCESSION B484p7o2x35s-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9565) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B474p7oM-LH* Fragment ends: XhoI and XbaI Fragment size: 8770 Insert molecule: 35s-Xba-xho* Fragment ends: XbaI and XhoI Fragment size: 795 Primer for gene synthesis 35sXba CCCGTCtctagaCGGGCCAACATGGT 35sXho GGGGCCCTCGAGAGAGATAGA Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 314..1098 /gene="P2x35s" /SECDrawAs="Region" /SECStyleId=1 CDS 1116..1349 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1350..1538 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 1539..1856 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1876..2166 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2342..2365 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2900..3597 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4146..7851 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8035..9045 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacg ggccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag 361 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa 421 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg 481 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct 541 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag 601 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact 661 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact 721 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac 781 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa 841 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc 901 acgaggagca tcgtggaaaa agaagacctt ccaaccacgt cttcaaagca agtggattga 961 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct 1021 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc 1081 tctacaaatc tatctctctc gagtcctcca ccgaaatggg ccccgagcgc aggcccgccg 1141 acatccgccg tgccaccgag gccgacatgc cagccgtctg caccatcgtg aaccactaca 1201 tcgagacctc cacggtcaac ttccgcacag agccgcagga gcctcaggag tggaccgacg 1261 acctcgtgag gctgcgcgag cgctacccct ggctcgtcgc tgaggtggat ggcgaggtcg 1321 ccggcatcgc ctacgcgggc ccctggaagg taagtttctg cttctacctt tgatatatat 1381 ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa 1441 gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata 1501 acttttctaa tatatgacca aaatttgttg atgtgcaggc acgcaacgcc tacgactgga 1561 cggcggagag caccgtctac gtgtccccac gccaccagcg caccggcctg ggctccacgc 1621 tctacaccca cctgctcaag agcctggagg cccagggctt caagtccgtg gtcgctgtga 1681 tcggcctccc caacgacccg agcgtccgta tgcacgaggc cctcggctac gcgccccgcg 1741 ggatgctgag ggccgcgggc ttcaagcacg gcaactggca cgacgtgggc ttctggcagc 1801 tcgacttctc cctgcctgtc ccaccccgcc cggtgctccc cgtcaccgaa atctgaactc 1861 cggaatcgcg ttcgagtatt atggcattgg gaaaactgtt tttcttgtac catttgttgt 1921 gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg 1981 atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg 2041 ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc aaacattttg 2101 ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa 2161 aacactgaag cctgcaggca tgcaagctga tccactagag gccatggcgg ccgcactagt 2221 ggatcccccg ggatgcatga attcaagctt atcgatgtcg acaggcctta agggccagat 2281 cttgggcccg gtacccgatc agattgtcgt ttcccgcctt cggtttaaac tatcagtgtt 2341 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat 2401 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca 2461 gggttcccct cgggagtgct tggcattccg tgcgataatg acttctgttc aaccacccaa 2521 acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga tcccttggct cgtctgggtc 2581 ggctagaagg tcgagtgggc tgctgtggct tgatccctca acgcggtcgc ggacgtagcg 2641 cagcgccgaa aaatcctcga tcgcaaatcc gacgctgtcg aaaagcgtga tctgcttgtc 2701 gctctttcgg ccgacgtcct ggccagtcat cacgcgccaa agttccgtca caggatgatc 2761 tggcgcgagt tgctggatct cgccttcaat ccgggtctgt ggcgggaact ccacgaaaat 2821 atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt cgccgccgac 2881 gccgttgatg tggacgccga aaaggatcta ggtgaagatc ctttttgata atctcatgac 2941 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 3001 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 3061 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 3121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 3181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 3241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 3301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 3361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 3421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 3481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 3541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 3601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 3661 ctttcctgcg ttatcccctg attctgtgga taaccgatta ccgcctttga gtgagctgat 3721 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 3781 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc 3841 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc 3901 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac 3961 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 4021 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcaggggtac gtcgaggtcg 4081 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct 4141 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc 4201 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg 4261 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc 4321 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag 4381 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt 4441 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc 4501 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc 4561 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc 4621 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag 4681 gccgccaagg cgcgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc 4741 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg 4801 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc 4861 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg 4921 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg 4981 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt 5041 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg 5101 atgccaagct cgcggcctgg ccggcgagct tggccgctga agaaaccgag cgccgccgtc 5161 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga 5221 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa 5281 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact 5341 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc 5401 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg 5461 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc 5521 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag 5581 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt 5641 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat 5701 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat 5761 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga 5821 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact 5881 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc 5941 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc 6001 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg 6061 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag 6121 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga 6181 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg 6241 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa 6301 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg 6361 ctgggtgatg acctggtgga gaagttgaag gcggcgcagg ccgcccagcg gcaacgcatc 6421 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa 6481 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag 6541 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc 6601 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc 6661 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggcgagtgtg 6721 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac 6781 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc 6841 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt 6901 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg 6961 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc 7021 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa 7081 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc 7141 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg 7201 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc 7261 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg 7321 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc 7381 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga 7441 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac 7501 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg 7561 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa 7621 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg 7681 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct 7741 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc 7801 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cggtgggtat 7861 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc 7921 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca 7981 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc 8041 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt 8101 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag 8161 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag 8221 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta 8281 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 8341 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat 8401 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 8461 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 8521 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 8581 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 8641 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 8701 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 8761 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 8821 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 8881 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag 8941 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt 9001 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt 9061 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata 9121 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat 9181 aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg ttatcgcaat 9241 agttggcgaa gtaatcgcaa catagcttgc ttggtcgttc cgcgtgaacg tcggctcgat 9301 tgtacctgcg ttcaaatact ttgcgatcgt gttgcgcgcc tgcccggtgc gtcggctgat 9361 ctcacggatc gactgcttct ctcgcaacgc catccgacgg atgatgttta aaagtcccat 9421 gtggatcact ccgttgcccc gtcgctcacc gtgttggggg gaaggtgcac atggctcagt 9481 tctcaatgga aattatctgc ctaaccggct cagttctgcg tagaaaccaa catgcaagct 9541 ccaccgggtg caaagcggca gcggc //