LOCUS B515p7o2x35s-Ubi 11397 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of 594pUbi-BA* into B484p7o2x35s-LH* ACCESSION B515p7o2x35s-Ubi KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11397) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B484p7o2x35s-LH* Fragment ends: SfiI Fragment size: 9501 Insert molecule: 594pUbi-BA* Fragment ends: SfiI Fragment size: 1896 Sequencing primers E9F GTGTCAAATCGTGGCCTCTAA SphR GGGTGGTTGAACAGAAGTCAT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 314..1098 /gene="P2x35s" /SECDrawAs="Region" /SECStyleId=1 CDS 1116..1349 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1350..1538 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 1539..1856 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1876..2166 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2230..3737 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3216..3735 /gene="int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3798..4051 /gene="Tnos" /product="NOS-T" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4174..4197 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4732..5429 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5978..9683 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9867..10877 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacg ggccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag 361 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa 421 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg 481 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct 541 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag 601 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact 661 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact 721 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac 781 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa 841 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc 901 acgaggagca tcgtggaaaa agaagacctt ccaaccacgt cttcaaagca agtggattga 961 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct 1021 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc 1081 tctacaaatc tatctctctc gagtcctcca ccgaaatggg ccccgagcgc aggcccgccg 1141 acatccgccg tgccaccgag gccgacatgc cagccgtctg caccatcgtg aaccactaca 1201 tcgagacctc cacggtcaac ttccgcacag agccgcagga gcctcaggag tggaccgacg 1261 acctcgtgag gctgcgcgag cgctacccct ggctcgtcgc tgaggtggat ggcgaggtcg 1321 ccggcatcgc ctacgcgggc ccctggaagg taagtttctg cttctacctt tgatatatat 1381 ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa 1441 gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata 1501 acttttctaa tatatgacca aaatttgttg atgtgcaggc acgcaacgcc tacgactgga 1561 cggcggagag caccgtctac gtgtccccac gccaccagcg caccggcctg ggctccacgc 1621 tctacaccca cctgctcaag agcctggagg cccagggctt caagtccgtg gtcgctgtga 1681 tcggcctccc caacgacccg agcgtccgta tgcacgaggc cctcggctac gcgccccgcg 1741 ggatgctgag ggccgcgggc ttcaagcacg gcaactggca cgacgtgggc ttctggcagc 1801 tcgacttctc cctgcctgtc ccaccccgcc cggtgctccc cgtcaccgaa atctgaactc 1861 cggaatcgcg ttcgagtatt atggcattgg gaaaactgtt tttcttgtac catttgttgt 1921 gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg 1981 atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg 2041 ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc aaacattttg 2101 ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa 2161 aacactgaag cctgcaggca tgcaagctga tccactagag gccatggggg ccaatttaaa 2221 tggcgtacgt gcagtgcagc gtgacccggt cgtgcccctc tctagagata atgagcattg 2281 catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt tgaagtgcag 2341 tttatctatc tttatacata tatttaaact ttactctacg aataatataa tctatagtac 2401 tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat ggtctaaagg 2461 acaattgagt attttgacaa caggactcta cagttttatc tttttagtgt gcatgtgttc 2521 tccttttttt ttgcaaatag cttcacctat ataatacttc atccatttta ttagtacatc 2581 catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca tctattttat 2641 tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt tatttaataa 2701 tttagatata aaatagaata aaataaagtg actaaaaatt aaacaaatac cctttaagaa 2761 attaaaaaaa ctaaggaaac atttttcttg tttcgagtag ataatgccag cctgttaaac 2821 gccgtcgatc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc 2881 aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc gagagttccg 2941 ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac 3001 gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc tacgggggat 3061 tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc 3121 tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct 3181 cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc 3241 cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac 3301 ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta 3361 cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt 3421 ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatctagg ataggtatac 3481 atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat ctattcatat 3541 gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa ttattttgat 3601 cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt tagccctgcc 3661 ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg 3721 gtgttacttc tgcaggtact agtggatccc ccgggctgca ggaattcaag cttacgcgtg 3781 tcgactcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc 3841 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa 3901 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc 3961 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat 4021 cgcgcgcggt gtcatctatg ttactagatc gctcgacgca tgctggtacc tgacgtctgc 4081 ggccgcatta attaaggccc taagggccag atcttgggcc cggtacccga tcagattgtc 4141 gtttcccgcc ttcggtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta 4201 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc 4261 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtg cttggcattc 4321 cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg acggagcagc 4381 attccaaaaa gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg gctgctgtgg 4441 cttgatccct caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc gatcgcaaat 4501 ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc ctggccagtc 4561 atcacgcgcc aaagttccgt cacaggatga tctggcgcga gttgctggat ctcgccttca 4621 atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat atcgcggtgc 4681 atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc gaaaaggatc 4741 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 4801 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 4861 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 4921 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 4981 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 5041 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 5101 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 5161 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 5221 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 5281 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 5341 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 5401 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 5461 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 5521 gataaccgat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 5581 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc 5641 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg 5701 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg 5761 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 5821 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 5881 gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc cctccgctgc tatagtgcag 5941 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag 6001 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc 6061 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg 6121 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac 6181 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca 6241 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga 6301 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc 6361 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca 6421 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc 6481 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcgcgaggc gtgaagtttg 6541 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg 6601 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc 6661 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc 6721 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg 6781 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat 6841 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt 6901 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct ggccggcgag 6961 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa 7021 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa 7081 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc 7141 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat 7201 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc 7261 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac 7321 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca 7381 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac 7441 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt 7501 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc 7561 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact 7621 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc 7681 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa 7741 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg 7801 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg 7861 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc 7921 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga 7981 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc 8041 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc 8101 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca 8161 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga 8221 aggcggcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt 8281 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc 8341 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct 8401 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga 8461 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg 8521 tttccgcagg gccggccggc atggcgagtg tgtgggatta cgacctggta ctgatggcgg 8581 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc 8641 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa 8701 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc 8761 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga 8821 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc 8881 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc 8941 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc 9001 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca 9061 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc 9121 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct 9181 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag 9241 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt 9301 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc 9361 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt 9421 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat 9481 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc 9541 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg 9601 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc 9661 gccggcgccc acatcaaggc accggtgggt atgcctgacg atgcgtggag accgaaacct 9721 tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg 9781 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg 9841 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg 9901 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg 9961 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt 10021 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa 10081 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg 10141 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg 10201 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg 10261 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag 10321 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt 10381 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg 10441 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa 10501 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg 10561 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact 10621 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa 10681 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc 10741 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct 10801 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca 10861 aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc 10921 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa 10981 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc 11041 atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc aacatagctt 11101 gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata ctttgcgatc 11161 gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga tcgactgctt ctctcgcaac 11221 gccatccgac ggatgatgtt taaaagtccc atgtggatca ctccgttgcc ccgtcgctca 11281 ccgtgttggg gggaaggtgc acatggctca gttctcaatg gaaattatct gcctaaccgg 11341 ctcagttctg cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggc //