LOCUS B487p5oAct-LH 11209 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of A373p6ido-Act* into B472p5oM-LH* ACCESSION B487p5oAct-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11209) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B472p5oM-LH* Fragment ends: XhoI and XbaI Fragment size: 9792 Insert molecule: A373p6ido-Act* Fragment ends: XbaI and XhoI Fragment size: 1417 GelR Sequencing primers Act1 CACTATCACCGTGAGTTGTC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 313..1710 /gene="ACT" /product="Actin Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 1738..2478 /gene="EPSPS" /translation="MAQINNMAQGIQTLNPNSNFHKPQVPKSSSFLVFGSKKLKNSAN SMLVLKKDSIFMQKFCSFRISASVATACMLHGASSRPATARKSSGLSGTVRIPGDKSI SHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLL APEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKS EDGDRLPVTLRGPKTPTPITYRVPMASAQ" /SECDrawAs="Gene" /SECStyleId=1 /SECName="epsps" misc_feature 2479..2667 /gene="int" /product="Intron STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron STLS1" CDS 2668..3510 /gene="epsps" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 3520..3810 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3986..4009 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4544..5241 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5790..9495 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9679..10689 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaca ggtcgaggtc attcatatgc ttgagaagag agtcgggata gtccaaaata 361 aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt ggtataaagt 421 aaaatatcgg taataaaagg tggcccaaag tgaaatttac tcttttctac tattataaaa 481 attgaggatg tttttgtcgg tactttgata cgtcattttt gtatgaattg gtttttaagt 541 ttattcgctt ttggaaatgc atatctgtat ttgagtcggg ttttaagttc gtttgctttt 601 gtaaatacag agggatttgt ataagaaata tctttaaaaa aacccatatg ctaatttgac 661 ataatttttg agaaaaatat atattcaggc gaattctcac aatgaacaat aataagatta 721 aaatagcttt cccccgttgc agcgcatggg tattttttct agtaaaaata aaagataaac 781 ttagactcaa aacatttaca aaaacaaccc ctaaagttcc taaagcccaa agtgctatcc 841 acgatccata gcaagcccag cccaacccaa cccaacccaa cccaccccag tccagccaac 901 tggacaatag tctccacacc cccccactat caccgtgagt tgtccgcacg caccgcacgt 961 ctcgcagcca aaaaaaaaaa aagaaagaaa aaaaagaaaa agaaaaaaca gcaggtgggt 1021 ccgggtcgtg ggggccggaa acgcgaggag gatcgcgagc cagcgacgag gccggccctc 1081 cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc cctctcctcc 1141 catcccccca accctaccac caccaccacc accacctcca cctcctcccc cctcgctgcc 1201 ggacgacgag ctcctccccc ctccccctcc gccgccgccg cgccggtaac caccccgccc 1261 ctctcctctt tctttctccg tttttttttt ccgtctcggt ctcgatcttt ggccttggta 1321 gtttgggtgg gcgagaggcg gcttcgtgcg cgcccagatc ggtgcgcggg aggggcggga 1381 tctcgcggct ggggctctcg ccggcgtgga tccggcccgg atctcgcggg gaatggggct 1441 ctcggatgta gatctgcgat ccgccgttgt tgggggagat gatggggggt ttaaaatttc 1501 cgccatgcta aacaagatca ggaagagggg aaaagggcac tatggtttat atttttatat 1561 atttctgctg cttcgtcagg cttagatgtg ctagatcttt ctttcttctt tttgtgggta 1621 gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt tgtgacaaat 1681 gcagcctcgt gcggagcttt tttgtaggta ctaggctagc tcgagtcctc caccgaaatg 1741 gcccagatca acaacatggc tcagggcatt cagaccctca accccaattc caacttccac 1801 aagccacaag tgcctaagtc tagctccttc ctcgtcttcg gctcgaagaa gttgaagaac 1861 agcgccaact ccatgcttgt tctcaagaaa gactcaatct tcatgcagaa gttttgcagc 1921 ttccgcatct ccgcaagtgt ggccactgct tgcatgttgc acggtgcctc cagccgtccg 1981 gcgaccgcca ggaagtcttc cggcctctcg gggaccgtca gaatccccgg agacaagagc 2041 atttcccacc gctcattcat gttcggcggc ctggccagcg gagagacaag gatcaccggc 2101 cttctggagg gtgaggatgt catcaacacc gggaaggcta tgcaggctat gggagcccgc 2161 atccggaagg aaggcgacac gtggattatc gacggcgtgg gaaacggcgg tctcctcgcc 2221 ccagaggcac ccttggactt cggcaacgcc gccaccggat gccgtctgac tatggggctt 2281 gttggcgtgt acgatttcga ctccaccttc atcggagacg cttccctcac caagcgccct 2341 atgggcaggg tcctaaatcc gctccgtgag atgggcgtgc aagtcaagag cgaggacgga 2401 gaccgcctgc cagttacctt gcgaggcccc aagactccaa cacccatcac ctacagggtg 2461 cctatggcct ctgcgcaggt aagtttctgc ttctaccttt gatatatata taataattat 2521 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat 2581 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat 2641 atatgaccaa aatttgttga tgtgcaggtc aagtccgccg tgctccttgc cgggctgaac 2701 actccgggta ttaccaccgt catcgagccc attatgacca gagatcatac cgagaagatg 2761 ctccagggat tcggcgctaa cttgacggtt gaaaccgacg ccgacggcgt gcgcaccatc 2821 cgtctggagg gaaggggcaa actcaccggc caagtcatcg acgtgccagg agatcccagc 2881 tcgactgctt ttccacttgt agccgccctc ttggtccctg gttccgacgt gaccatcctg 2941 aacgttctta tgaacccgac ccgcaccggg ctcatcttga ctctccagga gatgggcgct 3001 gacatcgaag tcattaaccc caggctggcc ggaggcgagg acgtggcaga tttgcgcgtc 3061 cgtagttcca ccctgaaggg tgtgacagtt ccagaggacc gggctcccag catgatcgac 3121 gagtacccta tccttgccgt cgccgcggcc ttcgccgagg gcgctaccgt gatgaacgga 3181 ttggaggagc tgcgcgtcaa ggagtcagac agactctccg ccgtggccaa tgggctcaag 3241 ctcaacggcg ttgactgcga tgagggagaa accagccttg tcgtgcgtgg caggccggac 3301 ggcaagggac tcggtaacgc ttctggggcc gcagtcgcca cgcacctgga ccaccgcatc 3361 gcgatgtcct tcctcgtgat gggccttgtt tcggagaacc cagtcaccgt ggacgatgcc 3421 accatgattg ctactagctt ccccgagttc atggacttga tggccggact gggagccaag 3481 atcgagctgt ccgacaccaa ggctgcctaa cgcgttcgag tattatggca ttgggaaaac 3541 tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc 3601 tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt 3661 tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat 3721 tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat 3781 gaccgaagtt aatatgagga gtaaaacact gaagcctgca ggcatgcaag ctgatccact 3841 agaggccatg gcggccgcac tagtggatcc cccgggatgc atgaattcaa gcttatcgat 3901 gtcgacaggc cttaagggcc agatcttggg cccggtaccc gatcagattg tcgtttcccg 3961 ccttcggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa 4021 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc 4081 atttgtatgt gcatgccaac cacagggttc ccctcgggag tgcttggcat tccgtgcgat 4141 aatgacttct gttcaaccac ccaaacgtcg gaaagcctga cgacggagca gcattccaaa 4201 aagatccctt ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc 4261 ctcaacgcgg tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct 4321 gtcgaaaagc gtgatctgct tgtcgctctt tcggccgacg tcctggccag tcatcacgcg 4381 ccaaagttcc gtcacaggat gatctggcgc gagttgctgg atctcgcctt caatccgggt 4441 ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt 4501 cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga tctaggtgaa 4561 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 4621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 4681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 4741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 4801 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 4861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 4921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 4981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 5041 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 5101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 5161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 5221 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 5281 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 5341 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 5401 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc 5461 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta 5521 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc 5581 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag 5641 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg 5701 cgaggcaggg gtacgtcgag gtcgatccaa cccctccgct gctatagtgc agtcggcttc 5761 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga 5821 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag 5881 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc 5941 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa 6001 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac 6061 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc 6121 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag 6181 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc 6241 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc 6301 gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt tggcccccgc 6361 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc 6421 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt 6481 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac 6541 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca 6601 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg 6661 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc 6721 atgaaatcct ggccggtttg tctgatgcca agctcgcggc ctggccggcg agcttggccg 6781 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc 6841 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg 6901 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac 6961 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc 7021 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg 7081 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt 7141 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt 7201 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga 7261 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc 7321 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga 7381 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc 7441 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct 7501 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa 7561 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg 7621 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat 7681 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct 7741 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt 7801 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg 7861 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc 7921 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc 7981 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg 8041 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg 8101 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt 8161 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg 8221 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac 8281 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca 8341 gggccggccg gcatggcgag tgtgtgggat tacgacctgg tactgatggc ggtttcccat 8401 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc 8461 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa 8521 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg 8581 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc 8641 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat 8701 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat 8761 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca 8821 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga 8881 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag 8941 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac 9001 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt 9061 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg 9121 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt 9181 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc 9241 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct 9301 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta 9361 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta 9421 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc 9481 ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg 9541 ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc 9601 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag 9661 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc 9721 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag 9781 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta 9841 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg 9901 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag 9961 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc 10021 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca 10081 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag 10141 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat 10201 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc 10261 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat 10321 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat 10381 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc 10441 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa 10501 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag 10561 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg 10621 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc 10681 ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca 10741 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt 10801 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg 10861 ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatagc ttgcttggtc 10921 gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg 10981 cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg 11041 acggatgatg tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg 11101 gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc 11161 tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcggc //