LOCUS B490p9oAct-LH 10437 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of A373p6ido-Act* into B475p9oM-LH* ACCESSION B490p9oAct-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10437) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B475p9oM-LH* Fragment ends: XhoI and XbaI Fragment size: 9020 Insert molecule: A373p6ido-Act* Fragment ends: XbaI and XhoI Fragment size: 1417 GelR Sequencing primers Act1 CACTATCACCGTGAGTTGTC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 313..1710 /gene="ACT" /product="Actin Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 1738..2036 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 CDS 2037..2223 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 CDS 2224..2730 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2748..3038 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3214..3237 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3772..4469 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5018..8723 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8907..9917 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaca ggtcgaggtc attcatatgc ttgagaagag agtcgggata gtccaaaata 361 aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt ggtataaagt 421 aaaatatcgg taataaaagg tggcccaaag tgaaatttac tcttttctac tattataaaa 481 attgaggatg tttttgtcgg tactttgata cgtcattttt gtatgaattg gtttttaagt 541 ttattcgctt ttggaaatgc atatctgtat ttgagtcggg ttttaagttc gtttgctttt 601 gtaaatacag agggatttgt ataagaaata tctttaaaaa aacccatatg ctaatttgac 661 ataatttttg agaaaaatat atattcaggc gaattctcac aatgaacaat aataagatta 721 aaatagcttt cccccgttgc agcgcatggg tattttttct agtaaaaata aaagataaac 781 ttagactcaa aacatttaca aaaacaaccc ctaaagttcc taaagcccaa agtgctatcc 841 acgatccata gcaagcccag cccaacccaa cccaacccaa cccaccccag tccagccaac 901 tggacaatag tctccacacc cccccactat caccgtgagt tgtccgcacg caccgcacgt 961 ctcgcagcca aaaaaaaaaa aagaaagaaa aaaaagaaaa agaaaaaaca gcaggtgggt 1021 ccgggtcgtg ggggccggaa acgcgaggag gatcgcgagc cagcgacgag gccggccctc 1081 cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc cctctcctcc 1141 catcccccca accctaccac caccaccacc accacctcca cctcctcccc cctcgctgcc 1201 ggacgacgag ctcctccccc ctccccctcc gccgccgccg cgccggtaac caccccgccc 1261 ctctcctctt tctttctccg tttttttttt ccgtctcggt ctcgatcttt ggccttggta 1321 gtttgggtgg gcgagaggcg gcttcgtgcg cgcccagatc ggtgcgcggg aggggcggga 1381 tctcgcggct ggggctctcg ccggcgtgga tccggcccgg atctcgcggg gaatggggct 1441 ctcggatgta gatctgcgat ccgccgttgt tgggggagat gatggggggt ttaaaatttc 1501 cgccatgcta aacaagatca ggaagagggg aaaagggcac tatggtttat atttttatat 1561 atttctgctg cttcgtcagg cttagatgtg ctagatcttt ctttcttctt tttgtgggta 1621 gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt tgtgacaaat 1681 gcagcctcgt gcggagcttt tttgtaggta ctaggctagc tcgagtcctc caccgaaatg 1741 ggagcatgga ttgagcagga tggcctccat gctggtagcc ccgctgcttg ggtggagcgc 1801 ctctttggct acgactgggc ccaacagacc attgggtgca gtgatgccgc tgtcttccgt 1861 ttgagcgccc agggcaggcc agttctcttc gtgaagactg acctctccgg tgccctcaac 1921 gagttgcagg acgaagctgc ccgcctctca tggcttgcca ccaccggagt cccttgcgct 1981 gccgtgttgg acgttgtcac agaggccgga agagactggc tgctcttggg cgaggtaagt 2041 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat 2101 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt 2161 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg 2221 caggtgccag gccaagacct cctgagctcc cacctggccc cagccgagaa ggtctccatc 2281 atggcagacg ccatgcgtcg cctccacacc ctggacccag ccacctgccc cttcgaccac 2341 caggccaagc acaggatcga acgtgcacgc actaggatgg aggccggcct tgtggaccag 2401 gatgaccttg acgaggagca ccagggcctt gctcccgccg agctgttcgc ccgcctcaag 2461 gctagaatgc ctgacggaga ggacttggtg gtcacccacg gggatgcctg cctccccaac 2521 atcatggttg agaacggacg cttctctggc ttcatcgact gcggtcgtct gggcgtggcc 2581 gaccgctacc aagacatcgc cctggccacc agggacatcg ctgaggaact tggaggggag 2641 tgggccgatc gtttcttggt cctctacggc attgctgccc ccgactccca gaggatcgcc 2701 ttctaccgcc tgcttgacga gttcttctga tccggaatcg cgttcgagta ttatggcatt 2761 gggaaaactg tttttcttgt accatttgtt gtgcttgtaa tttactgtgt tttttattcg 2821 gttttcgcta tcgaactgtg aaatggaaat ggatggagaa gagttaatga atgatatggt 2881 ccttttgttc attctcaaat taatattatt tgttttttct cttatttgtt gtgtgttgaa 2941 tttgaaatta taagagatat gcaaacattt tgttttgagt aaaaatgtgt caaatcgtgg 3001 cctctaatga ccgaagttaa tatgaggagt aaaacactga agcctgcagg catgcaagct 3061 gatccactag aggccatggc ggccgcacta gtggatcccc cgggatgcat gaattcaagc 3121 ttatcgatgt cgacaggcct taagggccag atcttgggcc cggtacccga tcagattgtc 3181 gtttcccgcc ttcggtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta 3241 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc 3301 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtg cttggcattc 3361 cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg acggagcagc 3421 attccaaaaa gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg gctgctgtgg 3481 cttgatccct caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc gatcgcaaat 3541 ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc ctggccagtc 3601 atcacgcgcc aaagttccgt cacaggatga tctggcgcga gttgctggat ctcgccttca 3661 atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat atcgcggtgc 3721 atctcggtct tgcctgggca gtcgccgccg acgccgttga tgtggacgcc gaaaaggatc 3781 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 3841 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 3901 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 3961 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 4021 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 4081 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 4141 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 4201 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 4261 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 4321 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 4381 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 4441 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 4501 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 4561 gataaccgat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 4621 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc 4681 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg 4741 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg 4801 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 4861 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 4921 gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc cctccgctgc tatagtgcag 4981 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag 5041 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc 5101 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg 5161 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac 5221 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca 5281 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga 5341 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc 5401 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca 5461 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc 5521 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcgcgaggc gtgaagtttg 5581 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg 5641 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc 5701 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc 5761 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg 5821 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat 5881 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt 5941 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct ggccggcgag 6001 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa 6061 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa 6121 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc 6181 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat 6241 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc 6301 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac 6361 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca 6421 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac 6481 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt 6541 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc 6601 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact 6661 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc 6721 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa 6781 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg 6841 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg 6901 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc 6961 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga 7021 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc 7081 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc 7141 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca 7201 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga 7261 aggcggcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt 7321 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc 7381 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct 7441 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga 7501 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg 7561 tttccgcagg gccggccggc atggcgagtg tgtgggatta cgacctggta ctgatggcgg 7621 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc 7681 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa 7741 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc 7801 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga 7861 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc 7921 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc 7981 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc 8041 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca 8101 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc 8161 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct 8221 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag 8281 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt 8341 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc 8401 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt 8461 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat 8521 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc 8581 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg 8641 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc 8701 gccggcgccc acatcaaggc accggtgggt atgcctgacg atgcgtggag accgaaacct 8761 tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg 8821 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg 8881 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg 8941 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg 9001 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt 9061 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa 9121 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg 9181 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg 9241 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg 9301 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag 9361 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt 9421 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg 9481 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa 9541 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg 9601 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact 9661 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa 9721 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc 9781 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct 9841 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca 9901 aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc 9961 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa 10021 gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc 10081 atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc aacatagctt 10141 gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata ctttgcgatc 10201 gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga tcgactgctt ctctcgcaac 10261 gccatccgac ggatgatgtt taaaagtccc atgtggatca ctccgttgcc ccgtcgctca 10321 ccgtgttggg gggaaggtgc acatggctca gttctcaatg gaaattatct gcctaaccgg 10381 ctcagttctg cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggc //