LOCUS B477p5oUZm-LH 11309 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of UbiZm-Bsa* into B472p5oM-LH* ACCESSION B477p5oUZm-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11309) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B472p5oM-LH* Fragment ends: XhoI and XbaI Fragment size: 9792 Insert molecule: UbiZm-Bsa* Fragment ends: BsaI Fragment size: 1517 Primer for gene synthesis BsaXba ccGGTCTCtctagatCTGCAGTGCAGCGT BsaXho ccGGTCTCctcgagGACCTGCAGAAGTAACAC Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC UF1 CGAGTAGATAATGCCAGCC FEATURES Location/Qualifiers CDS 62..1569 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 /SECName="UbiZm" /SECDescr="Promotor" misc_feature 1049..1568 /gene="int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 /SECName="int" /SECDescr="Intron" CDS 1590..2330 /gene="EPSPS" /translation="MAQINNMAQGIQTLNPNSNFHKPQVPKSSSFLVFGSKKLKNSAN SMLVLKKDSIFMQKFCSFRISASVATACMLHGASSRPATARKSSGLSGTVRIPGDKSI SHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLL APEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKS EDGDRLPVTLRGPKTPTPITYRVPMASAQ" /SECDrawAs="Gene" /SECStyleId=1 /SECName="epsps" misc_feature 2331..2519 /gene="int" /product="Intron STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron STLS1" CDS 2520..3362 /gene="epsps" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 3372..3662 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3838..3861 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4396..5093 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5642..9347 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9531..10541 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 11062..11085 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 ORIGIN 1 cgtgtctaca ttcacgtcca aatgggggct tagatgagaa acttcacgat cggctctaga 61 tctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct 121 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct 181 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata 241 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg 301 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt 361 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag 421 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt 481 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat 541 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa 601 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg 661 atcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa 721 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc 781 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc 841 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc 901 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac 961 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa 1021 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct 1081 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt 1141 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat 1201 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat 1261 cctgggatgg ctctagccgt tccgcagacg ggatcgatct aggataggta tacatgttga 1321 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa 1381 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata 1441 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac 1501 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac 1561 ttctgcaggt cctcgagtcc tccaccgaaa tggcccagat caacaacatg gctcagggca 1621 ttcagaccct caaccccaat tccaacttcc acaagccaca agtgcctaag tctagctcct 1681 tcctcgtctt cggctcgaag aagttgaaga acagcgccaa ctccatgctt gttctcaaga 1741 aagactcaat cttcatgcag aagttttgca gcttccgcat ctccgcaagt gtggccactg 1801 cttgcatgtt gcacggtgcc tccagccgtc cggcgaccgc caggaagtct tccggcctct 1861 cggggaccgt cagaatcccc ggagacaaga gcatttccca ccgctcattc atgttcggcg 1921 gcctggccag cggagagaca aggatcaccg gccttctgga gggtgaggat gtcatcaaca 1981 ccgggaaggc tatgcaggct atgggagccc gcatccggaa ggaaggcgac acgtggatta 2041 tcgacggcgt gggaaacggc ggtctcctcg ccccagaggc acccttggac ttcggcaacg 2101 ccgccaccgg atgccgtctg actatggggc ttgttggcgt gtacgatttc gactccacct 2161 tcatcggaga cgcttccctc accaagcgcc ctatgggcag ggtcctaaat ccgctccgtg 2221 agatgggcgt gcaagtcaag agcgaggacg gagaccgcct gccagttacc ttgcgaggcc 2281 ccaagactcc aacacccatc acctacaggg tgcctatggc ctctgcgcag gtaagtttct 2341 gcttctacct ttgatatata tataataatt atcattaatt agtagtaata taatatttca 2401 aatatttttt tcaaaataaa agaatgtagt atatagcaat tgcttttctg tagtttataa 2461 gtgtgtatat tttaatttat aacttttcta atatatgacc aaaatttgtt gatgtgcagg 2521 tcaagtccgc cgtgctcctt gccgggctga acactccggg tattaccacc gtcatcgagc 2581 ccattatgac cagagatcat accgagaaga tgctccaggg attcggcgct aacttgacgg 2641 ttgaaaccga cgccgacggc gtgcgcacca tccgtctgga gggaaggggc aaactcaccg 2701 gccaagtcat cgacgtgcca ggagatccca gctcgactgc ttttccactt gtagccgccc 2761 tcttggtccc tggttccgac gtgaccatcc tgaacgttct tatgaacccg acccgcaccg 2821 ggctcatctt gactctccag gagatgggcg ctgacatcga agtcattaac cccaggctgg 2881 ccggaggcga ggacgtggca gatttgcgcg tccgtagttc caccctgaag ggtgtgacag 2941 ttccagagga ccgggctccc agcatgatcg acgagtaccc tatccttgcc gtcgccgcgg 3001 ccttcgccga gggcgctacc gtgatgaacg gattggagga gctgcgcgtc aaggagtcag 3061 acagactctc cgccgtggcc aatgggctca agctcaacgg cgttgactgc gatgagggag 3121 aaaccagcct tgtcgtgcgt ggcaggccgg acggcaaggg actcggtaac gcttctgggg 3181 ccgcagtcgc cacgcacctg gaccaccgca tcgcgatgtc cttcctcgtg atgggccttg 3241 tttcggagaa cccagtcacc gtggacgatg ccaccatgat tgctactagc ttccccgagt 3301 tcatggactt gatggccgga ctgggagcca agatcgagct gtccgacacc aaggctgcct 3361 aacgcgttcg agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt 3421 gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg 3481 agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt 3541 ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt 3601 gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca 3661 ctgaagcctg caggcatgca agctgatcca ctagaggcca tggcggccgc actagtggat 3721 cccccgggat gcatgaattc aagcttatcg atgtcgacag gccttaaggg ccagatcttg 3781 ggcccggtac ccgatcagat tgtcgtttcc cgccttcggt ttaaactatc agtgtttgac 3841 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt 3901 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt 3961 tcccctcggg agtgcttggc attccgtgcg ataatgactt ctgttcaacc acccaaacgt 4021 cggaaagcct gacgacggag cagcattcca aaaagatccc ttggctcgtc tgggtcggct 4081 agaaggtcga gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac gtagcgcagc 4141 gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg cttgtcgctc 4201 tttcggccga cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg atgatctggc 4261 gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac gaaaatatcc 4321 gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg 4381 ttgatgtgga cgccgaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 4441 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 4501 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 4561 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 4621 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 4681 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 4741 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 4801 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 4861 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 4921 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 4981 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 5041 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 5101 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 5161 cctgcgttat cccctgattc tgtggataac cgattaccgc ctttgagtga gctgataccg 5221 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 5281 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc 5341 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg 5401 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 5461 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 5521 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag gggtacgtcg aggtcgatcc 5581 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa 5641 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc 5701 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac 5761 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg 5821 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt 5881 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc 5941 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc 6001 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg 6061 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg 6121 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg 6181 ccaaggcgcg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc 6241 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg 6301 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg 6361 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg 6421 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga 6481 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga 6541 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc 6601 caagctcgcg gcctggccgg cgagcttggc cgctgaagaa accgagcgcc gccgtctaaa 6661 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg 6721 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag 6781 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc 6841 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc 6901 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac 6961 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac 7021 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct 7081 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg 7141 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc 7201 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg 7261 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc 7321 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt 7381 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc 7441 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga 7501 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac 7561 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa 7621 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat 7681 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg 7741 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc 7801 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg 7861 gtgatgacct ggtggagaag ttgaaggcgg cgcaggccgc ccagcggcaa cgcatcgagg 7921 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc 7981 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac 8041 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg 8101 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg 8161 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcg agtgtgtggg 8221 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg 8281 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt 8341 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt 8401 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga 8461 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc 8521 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca 8581 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc 8641 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca 8701 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc 8761 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg 8821 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt 8881 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag 8941 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga 9001 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg 9061 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc 9121 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa 9181 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg 9241 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac 9301 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccggt gggtatgcct 9361 gacgatgcgt ggagaccgaa accttgcgct cgttcgccag ccaggacaga aatgcctcga 9421 cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa 9481 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac 9541 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca 9601 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag 9661 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg 9721 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga 9781 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg 9841 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc 9901 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt 9961 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg 10021 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag 10081 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc 10141 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg 10201 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa 10261 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt 10321 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact 10381 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt 10441 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc 10501 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa 10561 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg 10621 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc 10681 cctacacaaa ttgggagata tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt 10741 ggcgaagtaa tcgcaacata gcttgcttgg tcgttccgcg tgaacgtcgg ctcgattgta 10801 cctgcgttca aatactttgc gatcgtgttg cgcgcctgcc cggtgcgtcg gctgatctca 10861 cggatcgact gcttctctcg caacgccatc cgacggatga tgtttaaaag tcccatgtgg 10921 atcactccgt tgccccgtcg ctcaccgtgt tggggggaag gtgcacatgg ctcagttctc 10981 aatggaaatt atctgcctaa ccggctcagt tctgcgtaga aaccaacatg caagctccac 11041 cgggtgcaaa gcggcagcgg cggcaggata tattcaattg taaatggctt catgtccggg 11101 aaatctacat ggatcagcaa tgagtatgat ggtcaatatg gagaaaaaga aagagtaatt 11161 accaattttt tttcaattca aaaatgtaga tgtccgcagc gttattataa aatgaaagta 11221 cattttgata aaacgacaaa ttacgatccg tcgtatttat aggcgaaagc aataaacaaa 11281 ttattctaat tcggaaatct ttatttcga //