LOCUS B478p6oUZm-LH 10759 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of UbiZm-Bsa* into B473p6oM-LH* ACCESSION B478p6oUZm-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10759) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B473p6oM-LH* Fragment ends: XhoI and XbaI Fragment size: 9242 Insert molecule: UbiZm-Bsa* Fragment ends: BsaI Fragment size: 1517 Primer for gene synthesis BsaXba ccGGTCTCtctagatCTGCAGTGCAGCGT BsaXho ccGGTCTCctcgagGACCTGCAGAAGTAACAC Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC UF1 CGAGTAGATAATGCCAGCC FEATURES Location/Qualifiers CDS 62..1569 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 /SECName="UbiZm" /SECDescr="Promotor" misc_feature 1049..1568 /gene="int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 /SECName="int" /SECDescr="Intron" CDS 1590..2159 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 2160..2348 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 2349..2804 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2822..3112 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3288..3311 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3846..4543 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5092..8797 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8981..9991 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 10512..10535 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 ORIGIN 1 cgtgtctaca ttcacgtcca aatgggggct tagatgagaa acttcacgat cggctctaga 61 tctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct 121 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct 181 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata 241 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg 301 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt 361 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag 421 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt 481 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat 541 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa 601 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg 661 atcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa 721 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc 781 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc 841 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc 901 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac 961 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa 1021 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct 1081 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt 1141 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat 1201 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat 1261 cctgggatgg ctctagccgt tccgcagacg ggatcgatct aggataggta tacatgttga 1321 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa 1381 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata 1441 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac 1501 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac 1561 ttctgcaggt cctcgagtcc tccaccgaaa tgaagaaacc ggagctgacc gccacttccg 1621 tcgaaaagtt cctcatcgag aagtttgata gcgtgtctga ccttatgcag ttgtcggagg 1681 gcgaagagtc ccgcgcgttc agctttgatg ttggcgggcg cgggtacgtc ctccgcgtga 1741 actcttgcgc tgacggcttc tataaagatc ggtacgttta caggcatttc gcctcggcgg 1801 ccctgcctat tcccgaagtc ctcgacatcg gcgagttttc cgagagcctt acgtattgta 1861 tttcccgccg ggctcaaggg gtgaccctcc aggatctgcc ggaaactgag ttgcctgcgg 1921 ttctccagcc cgtcgccgag gctatggacg ccatcgccgc ggcggatctt tctcaaacgt 1981 cgggcttcgg gccgttcggc cctcagggga ttggccagta caccacttgg agggacttta 2041 tctgcgctat cgccgatccg cacgtgtatc attggcaaac cgttatggac gatacggtct 2101 ccgcgagcgt ggcccaggct ctggacgaac tcatgttgtg ggccgaggat tgccccgagg 2161 taagtttctg cttctacctt tgatatatat ataataatta tcattaatta gtagtaatat 2221 aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt gcttttctgt 2281 agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca aaatttgttg 2341 atgtgcaggt ccgccacctg gttcatgcgg acttcggctc taataacgtg ctcaccgata 2401 atgggcggat tactgccgtc atcgactggt ccgaagctat gtttggcgat tcgcagtacg 2461 aagtggcgaa cattttcttc tggaggcctt ggcttgcctg catggaacag caaacgcgct 2521 actttgagcg gaggcacccg gagctggcgg ggtctccccg cctcagggcc tatatgttgc 2581 ggatcggcct cgaccagctt taccagtccc tggttgatgg gaatttcgac gatgctgcct 2641 gggcgcaagg ccgctgtgac gctattgtca ggagcggcgc ggggaccgtg ggccgcactc 2701 agatcgcccg gcggtcggct gccgtttgga cggatgggtg cgtcgaagtg ctcgcggaca 2761 gcggcaacag gcggccttcc acccgcccgc gggccaagga gtgatccgga atcgcgttcg 2821 agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact 2881 gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta 2941 atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt 3001 tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat 3061 gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca ctgaagcctg 3121 caggcatgca agctgatcca ctagaggcca tggcggccgc actagtggat cccccgggat 3181 gcatgaattc aagcttatcg atgtcgacag gccttaaggg ccagatcttg ggcccggtac 3241 ccgatcagat tgtcgtttcc cgccttcggt ttaaactatc agtgtttgac aggatatatt 3301 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt 3361 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg 3421 agtgcttggc attccgtgcg ataatgactt ctgttcaacc acccaaacgt cggaaagcct 3481 gacgacggag cagcattcca aaaagatccc ttggctcgtc tgggtcggct agaaggtcga 3541 gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac gtagcgcagc gccgaaaaat 3601 cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg cttgtcgctc tttcggccga 3661 cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg atgatctggc gcgagttgct 3721 ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac gaaaatatcc gaacgcagca 3781 agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg ttgatgtgga 3841 cgccgaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 3901 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 3961 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 4021 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 4081 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 4141 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 4201 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 4261 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 4321 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 4381 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 4441 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 4501 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 4561 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 4621 cccctgattc tgtggataac cgattaccgc ctttgagtga gctgataccg ctcgccgcag 4681 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta 4741 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat 4801 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc 4861 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc 4921 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt 4981 tcaccgtcat caccgaaacg cgcgaggcag gggtacgtcg aggtcgatcc aacccctccg 5041 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc 5101 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt 5161 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat 5221 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga 5281 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa 5341 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc 5401 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact 5461 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg 5521 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga 5581 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcgcg 5641 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga 5701 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg 5761 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg 5821 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa 5881 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc 5941 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg 6001 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctcgcg 6061 gcctggccgg cgagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt 6121 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat 6181 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt 6241 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg 6301 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag 6361 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca 6421 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt 6481 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat 6541 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc 6601 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg 6661 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga 6721 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg 6781 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg 6841 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg 6901 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa 6961 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa 7021 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg 7081 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac 7141 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa 7201 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg 7261 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct 7321 ggtggagaag ttgaaggcgg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg 7381 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc 7441 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt 7501 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt 7561 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga 7621 cgggcacgta gaggtttccg cagggccggc cggcatggcg agtgtgtggg attacgacct 7681 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg 7741 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg 7801 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac 7861 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga 7921 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta 7981 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga 8041 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta 8101 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta 8161 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat 8221 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat 8281 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac 8341 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc 8401 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat 8461 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga 8521 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg 8581 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac 8641 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg 8701 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc 8761 gtcgccactc gaccgccggc gcccacatca aggcaccggt gggtatgcct gacgatgcgt 8821 ggagaccgaa accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct 8881 gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac 8941 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc 9001 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta 9061 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc 9121 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc 9181 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc 9241 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta 9301 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt 9361 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc 9421 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga 9481 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg 9541 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt 9601 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt 9661 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa 9721 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg 9781 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga 9841 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca 9901 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa 9961 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg 10021 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca 10081 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa 10141 ttgggagata tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa 10201 tcgcaacata gcttgcttgg tcgttccgcg tgaacgtcgg ctcgattgta cctgcgttca 10261 aatactttgc gatcgtgttg cgcgcctgcc cggtgcgtcg gctgatctca cggatcgact 10321 gcttctctcg caacgccatc cgacggatga tgtttaaaag tcccatgtgg atcactccgt 10381 tgccccgtcg ctcaccgtgt tggggggaag gtgcacatgg ctcagttctc aatggaaatt 10441 atctgcctaa ccggctcagt tctgcgtaga aaccaacatg caagctccac cgggtgcaaa 10501 gcggcagcgg cggcaggata tattcaattg taaatggctt catgtccggg aaatctacat 10561 ggatcagcaa tgagtatgat ggtcaatatg gagaaaaaga aagagtaatt accaattttt 10621 tttcaattca aaaatgtaga tgtccgcagc gttattataa aatgaaagta cattttgata 10681 aaacgacaaa ttacgatccg tcgtatttat aggcgaaagc aataaacaaa ttattctaat 10741 tcggaaatct ttatttcga //