LOCUS B479p7oUZm-LH 10287 bp DNA circular SYN 26-JAN-2016 DEFINITION Ligation of UbiZm-Bsa* into B474p7oM-LH* ACCESSION B479p7oUZm-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10287) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B474p7oM-LH* Fragment ends: XhoI and XbaI Fragment size: 8770 Insert molecule: UbiZm-Bsa* Fragment ends: BsaI Fragment size: 1517 Primer for gene synthesis BsaXba ccGGTCTCtctagatCTGCAGTGCAGCGT BsaXho ccGGTCTCctcgagGACCTGCAGAAGTAACAC Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC UF1 CGAGTAGATAATGCCAGCC FEATURES Location/Qualifiers CDS 62..1569 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 /SECName="UbiZm" /SECDescr="Promotor" misc_feature 1049..1568 /gene="int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 /SECName="int" /SECDescr="Intron" CDS 1590..1823 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1824..2012 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 2013..2330 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2350..2640 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2816..2839 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3374..4071 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4620..8325 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8509..9519 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 10040..10063 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 ORIGIN 1 cgtgtctaca ttcacgtcca aatgggggct tagatgagaa acttcacgat cggctctaga 61 tctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct 121 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct 181 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata 241 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg 301 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt 361 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag 421 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt 481 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat 541 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa 601 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg 661 atcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa 721 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc 781 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc 841 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc 901 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac 961 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa 1021 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct 1081 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt 1141 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat 1201 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat 1261 cctgggatgg ctctagccgt tccgcagacg ggatcgatct aggataggta tacatgttga 1321 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa 1381 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata 1441 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac 1501 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac 1561 ttctgcaggt cctcgagtcc tccaccgaaa tgggccccga gcgcaggccc gccgacatcc 1621 gccgtgccac cgaggccgac atgccagccg tctgcaccat cgtgaaccac tacatcgaga 1681 cctccacggt caacttccgc acagagccgc aggagcctca ggagtggacc gacgacctcg 1741 tgaggctgcg cgagcgctac ccctggctcg tcgctgaggt ggatggcgag gtcgccggca 1801 tcgcctacgc gggcccctgg aaggtaagtt tctgcttcta cctttgatat atatataata 1861 attatcatta attagtagta atataatatt tcaaatattt ttttcaaaat aaaagaatgt 1921 agtatatagc aattgctttt ctgtagttta taagtgtgta tattttaatt tataactttt 1981 ctaatatatg accaaaattt gttgatgtgc aggcacgcaa cgcctacgac tggacggcgg 2041 agagcaccgt ctacgtgtcc ccacgccacc agcgcaccgg cctgggctcc acgctctaca 2101 cccacctgct caagagcctg gaggcccagg gcttcaagtc cgtggtcgct gtgatcggcc 2161 tccccaacga cccgagcgtc cgtatgcacg aggccctcgg ctacgcgccc cgcgggatgc 2221 tgagggccgc gggcttcaag cacggcaact ggcacgacgt gggcttctgg cagctcgact 2281 tctccctgcc tgtcccaccc cgcccggtgc tccccgtcac cgaaatctga actccggaat 2341 cgcgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt 2401 aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag 2461 aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt 2521 ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga 2581 gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact 2641 gaagcctgca ggcatgcaag ctgatccact agaggccatg gcggccgcac tagtggatcc 2701 cccgggatgc atgaattcaa gcttatcgat gtcgacaggc cttaagggcc agatcttggg 2761 cccggtaccc gatcagattg tcgtttcccg ccttcggttt aaactatcag tgtttgacag 2821 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa 2881 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc 2941 ccctcgggag tgcttggcat tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg 3001 gaaagcctga cgacggagca gcattccaaa aagatccctt ggctcgtctg ggtcggctag 3061 aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc 3121 cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt 3181 tcggccgacg tcctggccag tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc 3241 gagttgctgg atctcgcctt caatccgggt ctgtggcggg aactccacga aaatatccga 3301 acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt 3361 gatgtggacg ccgaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 3421 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 3481 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 3541 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 3601 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 3661 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 3721 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 3781 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 3841 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 3901 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 3961 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4021 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4081 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 4141 tgcgttatcc cctgattctg tggataaccg attaccgcct ttgagtgagc tgataccgct 4201 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg 4261 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc 4321 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg 4381 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 4441 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 4501 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg gtacgtcgag gtcgatccaa 4561 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac 4621 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt 4681 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat 4741 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac 4801 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt 4861 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac 4921 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc 4981 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca 5041 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc 5101 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc 5161 aaggcgcgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac 5221 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc 5281 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag 5341 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc 5401 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac 5461 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc 5521 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca 5581 agctcgcggc ctggccggcg agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa 5641 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat 5701 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa 5761 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg 5821 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt 5881 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt 5941 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt 6001 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta 6061 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga 6121 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg 6181 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca 6241 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga 6301 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg 6361 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg 6421 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag 6481 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc 6541 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg 6601 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca 6661 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc 6721 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga 6781 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt 6841 gatgacctgg tggagaagtt gaaggcggcg caggccgccc agcggcaacg catcgaggca 6901 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg 6961 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca 7021 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac 7081 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag 7141 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggcgag tgtgtgggat 7201 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa 7261 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc 7321 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta 7381 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg 7441 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg 7501 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag 7561 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt 7621 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag 7681 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc 7741 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct 7801 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc 7861 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt 7921 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac 7981 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat 8041 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt 8101 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa 8161 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg 8221 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa 8281 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga 8341 cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact 8401 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc 8461 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc 8521 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg 8581 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg 8641 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc 8701 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact 8761 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta 8821 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg 8881 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg 8941 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt 9001 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa 9061 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg 9121 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag 9181 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg 9241 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc 9301 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg 9361 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat 9421 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac 9481 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc 9541 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct 9601 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc 9661 tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg 9721 cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc 9781 tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg 9841 gatcgactgc ttctctcgca acgccatccg acggatgatg tttaaaagtc ccatgtggat 9901 cactccgttg ccccgtcgct caccgtgttg gggggaaggt gcacatggct cagttctcaa 9961 tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa ccaacatgca agctccaccg 10021 ggtgcaaagc ggcagcggcg gcaggatata ttcaattgta aatggcttca tgtccgggaa 10081 atctacatgg atcagcaatg agtatgatgg tcaatatgga gaaaaagaaa gagtaattac 10141 caattttttt tcaattcaaa aatgtagatg tccgcagcgt tattataaaa tgaaagtaca 10201 ttttgataaa acgacaaatt acgatccgtc gtatttatag gcgaaagcaa taaacaaatt 10261 attctaattc ggaaatcttt atttcga //