LOCUS B480p9oUZm-LH 10537 bp DNA circular SYN 26-JAN-2016 DEFINITION Ligation of UbiZm-Bsa* into B475p9oM-LH* ACCESSION B480p9oUZm-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10537) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B475p9oM-LH* Fragment ends: XhoI and XbaI Fragment size: 9020 Insert molecule: UbiZm-Bsa* Fragment ends: BsaI Fragment size: 1517 Primer for gene synthesis BsaXba ccGGTCTCtctagatCTGCAGTGCAGCGT BsaXho ccGGTCTCctcgagGACCTGCAGAAGTAACAC Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC UF1 CGAGTAGATAATGCCAGCC FEATURES Location/Qualifiers CDS 62..1569 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 /SECName="UbiZm" /SECDescr="Promotor" misc_feature 1049..1568 /gene="int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 /SECName="int" /SECDescr="Intron" CDS 1590..1888 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 CDS 1889..2075 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 CDS 2076..2582 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2600..2890 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3066..3089 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3624..4321 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4870..8575 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8759..9769 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 10290..10313 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 ORIGIN 1 cgtgtctaca ttcacgtcca aatgggggct tagatgagaa acttcacgat cggctctaga 61 tctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct 121 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct 181 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata 241 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg 301 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt 361 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag 421 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt 481 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat 541 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa 601 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg 661 atcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa 721 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc 781 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc 841 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc 901 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac 961 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa 1021 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct 1081 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt 1141 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat 1201 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat 1261 cctgggatgg ctctagccgt tccgcagacg ggatcgatct aggataggta tacatgttga 1321 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa 1381 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata 1441 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac 1501 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac 1561 ttctgcaggt cctcgagtcc tccaccgaaa tgggagcatg gattgagcag gatggcctcc 1621 atgctggtag ccccgctgct tgggtggagc gcctctttgg ctacgactgg gcccaacaga 1681 ccattgggtg cagtgatgcc gctgtcttcc gtttgagcgc ccagggcagg ccagttctct 1741 tcgtgaagac tgacctctcc ggtgccctca acgagttgca ggacgaagct gcccgcctct 1801 catggcttgc caccaccgga gtcccttgcg ctgccgtgtt ggacgttgtc acagaggccg 1861 gaagagactg gctgctcttg ggcgaggtaa gtttctgctt ctacctttga tatatatata 1921 ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa 1981 tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact 2041 tttctaatat atgaccaaaa tttgttgatg tgcaggtgcc aggccaagac ctcctgagct 2101 cccacctggc cccagccgag aaggtctcca tcatggcaga cgccatgcgt cgcctccaca 2161 ccctggaccc agccacctgc cccttcgacc accaggccaa gcacaggatc gaacgtgcac 2221 gcactaggat ggaggccggc cttgtggacc aggatgacct tgacgaggag caccagggcc 2281 ttgctcccgc cgagctgttc gcccgcctca aggctagaat gcctgacgga gaggacttgg 2341 tggtcaccca cggggatgcc tgcctcccca acatcatggt tgagaacgga cgcttctctg 2401 gcttcatcga ctgcggtcgt ctgggcgtgg ccgaccgcta ccaagacatc gccctggcca 2461 ccagggacat cgctgaggaa cttggagggg agtgggccga tcgtttcttg gtcctctacg 2521 gcattgctgc ccccgactcc cagaggatcg ccttctaccg cctgcttgac gagttcttct 2581 gatccggaat cgcgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg 2641 ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa 2701 atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta 2761 tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat 2821 tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga 2881 gtaaaacact gaagcctgca ggcatgcaag ctgatccact agaggccatg gcggccgcac 2941 tagtggatcc cccgggatgc atgaattcaa gcttatcgat gtcgacaggc cttaagggcc 3001 agatcttggg cccggtaccc gatcagattg tcgtttcccg ccttcggttt aaactatcag 3061 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc 3121 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac 3181 cacagggttc ccctcgggag tgcttggcat tccgtgcgat aatgacttct gttcaaccac 3241 ccaaacgtcg gaaagcctga cgacggagca gcattccaaa aagatccctt ggctcgtctg 3301 ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt 3361 agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct 3421 tgtcgctctt tcggccgacg tcctggccag tcatcacgcg ccaaagttcc gtcacaggat 3481 gatctggcgc gagttgctgg atctcgcctt caatccgggt ctgtggcggg aactccacga 3541 aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc 3601 cgacgccgtt gatgtggacg ccgaaaagga tctaggtgaa gatccttttt gataatctca 3661 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 3721 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 3781 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 3841 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 3901 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 3961 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 4021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 4081 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 4141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 4201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 4261 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 4321 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 4381 tgttctttcc tgcgttatcc cctgattctg tggataaccg attaccgcct ttgagtgagc 4441 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 4501 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg 4561 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta 4621 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc 4681 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 4741 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg gtacgtcgag 4801 gtcgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc 4861 ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt 4921 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa 4981 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt 5041 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac 5101 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat 5161 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg 5221 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg 5281 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt 5341 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg 5401 cgaggccgcc aaggcgcgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca 5461 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc 5521 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac 5581 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc 5641 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc 5701 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac 5761 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg 5821 tctgatgcca agctcgcggc ctggccggcg agcttggccg ctgaagaaac cgagcgccgc 5881 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat 5941 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac 6001 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc 6061 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt 6121 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg 6181 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg 6241 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc 6301 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg 6361 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc 6421 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc 6481 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat 6541 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa 6601 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc 6661 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc 6721 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta 6781 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc 6841 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca 6901 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg 6961 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc 7021 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc 7081 ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg caggccgccc agcggcaacg 7141 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa 7201 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga 7261 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag 7321 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat 7381 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggcgag 7441 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg 7501 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt 7561 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg 7621 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg 7681 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga 7741 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac 7801 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg 7861 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg 7921 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt 7981 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc 8041 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc 8101 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg 8161 tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg 8221 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta 8281 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat 8341 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga 8401 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg 8461 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg 8521 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccggtgg 8581 gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa 8641 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa 8701 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat 8761 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc 8821 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa 8881 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac 8941 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga 9001 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt 9061 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat 9121 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa 9181 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt 9241 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca 9301 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga 9361 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc 9421 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga 9481 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct 9541 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc 9601 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag 9661 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga 9721 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat 9781 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca 9841 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca 9901 taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg 9961 caatagttgg cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg aacgtcggct 10021 cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg gtgcgtcggc 10081 tgatctcacg gatcgactgc ttctctcgca acgccatccg acggatgatg tttaaaagtc 10141 ccatgtggat cactccgttg ccccgtcgct caccgtgttg gggggaaggt gcacatggct 10201 cagttctcaa tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa ccaacatgca 10261 agctccaccg ggtgcaaagc ggcagcggcg gcaggatata ttcaattgta aatggcttca 10321 tgtccgggaa atctacatgg atcagcaatg agtatgatgg tcaatatgga gaaaaagaaa 10381 gagtaattac caattttttt tcaattcaaa aatgtagatg tccgcagcgt tattataaaa 10441 tgaaagtaca ttttgataaa acgacaaatt acgatccgtc gtatttatag gcgaaagcaa 10501 taaacaaatt attctaattc ggaaatcttt atttcga //