LOCUS B648pUbiZm-LRFPi 5634 bp DNA circular SYN 08-FEB-2016 DEFINITION Ligation of RFPi* into B633pUbiZm-LGFP* ACCESSION B648pUbiZm-LRFPi KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5634) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B633pUbiZm-LGFP* Fragment ends: SalI and MluI Fragment size: 4668 Insert molecule: RFPi* Fragment ends: MluI and SalI Fragment size: 966 Genesynthesis Sequencing primers Ubi1 CTGTTTCTTTTGTCGATGC FEATURES Location/Qualifiers misc_feature 106..1614 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1093..1612 /gene="int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1664..1714 /gene="link" /SECDrawAs="Region" /SECStyleId=1 CDS 1715..1860 /gene="TagRFP'" /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQT MRIK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="tagrfp" CDS 1861..2047 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 2048..2617 /gene="'TagRFP" /codon_start=2 /translation="LSREAHCLSPSTFWLRLSCTAHVPSSTIPKESRTSLSSPSPRAS PGKG" /SECDrawAs="Gene" /SECStyleId=1 /SECName="tagrfp" misc_feature 2623..2818 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3634..4494 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 4537..5253 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctaggctgca gtgcagcgtg 121 acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta 181 ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat 241 ttaaacttta ctctacgaat aatataatct atagtactac aataatatca gtgttttaga 301 gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag 361 gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt 421 cacctatata atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg 481 tttttataga ctaatttttt tagtacatct attttattct attttagcct ctaaattaag 541 aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa 601 taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt 661 tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgatcgac gagtctaacg 721 gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat 781 ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc 841 tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc 901 tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct 961 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct 1021 cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac 1081 ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc 1141 ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat 1201 ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag 1261 acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag 1321 ccgttccgca gacgggatcg atctaggata ggtatacatg ttgatgtggg ttttactgat 1381 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct 1441 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca 1501 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg 1561 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggtactagt 1621 tggatccccc gggctgcagg aattcaagct tacgcgttgt acaggagtac cgccccgtcc 1681 ggtcctgccc gtcaccgaga tcagcggaga gttcatggtc tccaagggcg aggagcttat 1741 caaggagaac atgcacatga agctctacat ggaaggaacc gtgaacaatc accacttcaa 1801 atgcaccagc gagggtgagg gcaagcccta cgaggggact cagaccatgc gcatcaaggt 1861 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata 1921 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta 1981 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga 2041 tgtgcaggtt gtcgagggag gcccattgcc tttcgccttc gacattctgg ctacgtcttt 2101 catgtacggc tcacgtacct tcatcaacca tacccaagga atcccggact tctttaagca 2161 gtccttcccc gagggcttca cctgggaaag ggtgacaacc tacgaggatg gtggagtcct 2221 cactgccacc caggacacca gcctccagga cggctgcttg atctacaacg tgaagattcg 2281 cggggttaac ttcccatcga acggccccgt catgcaaaag aagaccctgg gatgggaggc 2341 aaacacggag atgctctatc ctgccgacgg cggacttgag ggcagaaccg acatggcgtt 2401 gaagctcgtg ggtggcgggc acctgatctg caatttcaag actacctacc gctccaagaa 2461 gccagccaag aacctcaaga tgccgggagt ctactacgtg gatcaccgtc ttgagaggat 2521 caaggaagcc gacaaggaga cctacgttga gcagcacgag gtcgctgtgg cccgctactg 2581 cgacttgccc agcaagctgg gccacaagct caactgagtc gaccctgctt taatgagata 2641 tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa 2701 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat 2761 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtcctc 2821 gacaggcctt aagggccaga tcttgggccc ggtacccaat tcgccctata gtgagtcgta 2881 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 2941 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 3001 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt 3061 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 3121 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 3181 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 3241 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 3301 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 3361 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 3421 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 3481 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 3541 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 3601 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 3661 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 3721 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 3781 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 3841 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 3901 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 3961 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 4021 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 4081 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 4141 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 4201 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 4261 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 4321 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 4381 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 4441 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 4501 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 4561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 4621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 4681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 4741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 4801 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 4861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 4921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 4981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 5041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 5101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 5161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 5221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 5281 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 5341 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 5401 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 5461 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 5521 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 5581 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca attt //