LOCUS B726p35s-YFPD 4303 bp DNA circular SYN 26-JAN-2016 DEFINITION Ligation of cit-DCS-Mlu-Sal* into B589p35s-mGFPD-ocs* ACCESSION B726p35s-YFPD KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4303) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B589p35s-mGFPD-ocs* Fragment ends: SalI and MluI Fragment size: 3563 Insert molecule: cit-DCS-Mlu-Sal* Fragment ends: MluI and SalI Fragment size: 740 Genesynthesis Sequencing primers: 35S GCACAATCCCACTATCCTT FEATURES Location/Qualifiers misc_feature 144..562 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" CDS 590..1309 /gene="yfp" /product="mCtrine (codon optimized for plants)" /SECDrawAs="Gene" /SECStyleId=1 /SECName="yfp" /SECDescr="mCtrine (codon optimized for plants)" CDS 972..1309 /gene="cit" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1318..1513 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2329..3189 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 3232..3948 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg 121 gccgcactag tggatccccc gggtcaacat ggtggagcac gacactctcg tctactccaa 181 gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt 241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac 301 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt 361 tcaagatgcc tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt 421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac 481 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg 541 aagttcattt catttggaga ggacacaagc ttacgcgtcc tccataacta tggtgtccaa 601 gggcgaggag ctgttcaccg gagtcgttcc catcctcgtc gaattggacg gcgacgtgaa 661 cggtcacaag ttcagcgtgt ctggggaggg cgagggagat gccacctacg gcaagctcac 721 tctcaagttc atttgcacca ccggaaagct gccagtccct tggccgaccc tcgttactac 781 cttcggctac ggtttgatgt gcttcgctcg ctaccccgac cacatgaagc agcacgactt 841 ctttaagtcc gccatgccag agggctacgt ccaagagagg accatcttct tcaaggacga 901 tgggaactac aagacccgcg ccgaagtgaa gttcgagggc gacaccctgg tgaaccgcat 961 cgagctgaaa ggtatcgatt tcaaggaaga tggaaatatt ctcggacaca agttggagta 1021 caactacaac agccataacg tgtatatcat ggctgacaag cagaagaacg gcatcaaagt 1081 caacttcaag atcaggcaca atattgagga tggttcggtg cagctcgccg accactacca 1141 gcaaaacact cctatcgggg acggcccggt tcttctcccc gacaaccact acctgtccta 1201 ccagagcgcc ctctccaagg acccaaacga aaagcgcgat cacatggtct tgcttgagtt 1261 cgtgaccgct gccggaatca ccctgggcat ggacgagctt tacaagtgac tcgtcgaccc 1321 tgctttaatg agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg 1381 tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca 1441 ttctaatgaa tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat 1501 ttactgattg tcctcgacag gccttaaggg ccagatcttg ggcccggtac ccaattcgcc 1561 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa 1621 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 1681 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 1741 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 1801 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 1861 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 1921 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 1981 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 2041 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 2101 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 2161 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 2221 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 2281 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 2341 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 2401 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 2461 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 2521 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 2581 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 2641 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 2701 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 2761 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 2821 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 2881 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 2941 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 3001 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 3061 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 3121 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 3181 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 3241 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 3301 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 3361 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 3421 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 3481 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 3541 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 3601 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 3661 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 3721 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 3781 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 3841 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 3901 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 3961 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 4021 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 4081 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 4141 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 4201 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 4261 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgt //