LOCUS B727p35s-YFPiD 4492 bp DNA circular SYN 26-JAN-2016 DEFINITION Ligation of YFPi-Mlu-Sal* into B589p35s-mGFPD-ocs* ACCESSION B727p35s-YFPiD KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4492) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B589p35s-mGFPD-ocs* Fragment ends: SalI and MluI Fragment size: 3563 Insert molecule: YFPi-Mlu-Sal* Fragment ends: MluI and SalI Fragment size: 929 Primer for gene synthesis 35F CACTATCCTTCGCAAGACCCTTCCTCTA YFPR1 caaaggtagaagcagaaacttaCCTTTCAGCTCGATG STLS GGTAAGTTTCTGCTTCTACCT Sir CCTGCACATCAACAAATTTTG YFPF3 gaccaaaatttgttgatgtgcaGGTATCGATTTCAAGGAAG OcsR GCGATCATAGGCGTCTCGCATATCTC Sequencing primers: 35S GCACAATCCCACTATCCTT FEATURES Location/Qualifiers misc_feature 144..562 /gene="P35S" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" CDS 590..971 /gene="YFP'" /product="mCtrine (codon optimized for plants)" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="yfp" /SECDescr="mCitrine (codon optimized for plants)" CDS 972..1160 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 1161..1498 /gene="'YFP" /product="mCtrine (codon optimized for plants)" /codon_start=2 /translation="YRFQGRWKYSRTQVGVQLQQP" /SECDrawAs="Gene" /SECStyleId=1 /SECName="yfp" /SECDescr="mCitrine (codon optimized for plants)" misc_feature 1507..1702 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2518..3378 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 3421..4137 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg 121 gccgcactag tggatccccc gggtcaacat ggtggagcac gacactctcg tctactccaa 181 gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt 241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac 301 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt 361 tcaagatgcc tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt 421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac 481 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg 541 aagttcattt catttggaga ggacacaagc ttacgcgtcc tccataacta tggtgtccaa 601 gggcgaggag ctgttcaccg gagtcgttcc catcctcgtc gaattggacg gcgacgtgaa 661 cggtcacaag ttcagcgtgt ctggggaggg cgagggagat gccacctacg gcaagctcac 721 tctcaagttc atttgcacca ccggaaagct gccagtccct tggccgaccc tcgttactac 781 cttcggctac ggtttgatgt gcttcgctcg ctaccccgac cacatgaagc agcacgactt 841 ctttaagtcc gccatgccag agggctacgt ccaagagagg accatcttct tcaaggacga 901 tgggaactac aagacccgcg ccgaagtgaa gttcgagggc gacaccctgg tgaaccgcat 961 cgagctgaaa ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat 1021 tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa 1081 ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac 1141 caaaatttgt tgatgtgcag gtatcgattt caaggaagat ggaaatattc tcggacacaa 1201 gttggagtac aactacaaca gccataacgt gtatatcatg gctgacaagc agaagaacgg 1261 catcaaagtc aacttcaaga tcaggcacaa tattgaggat ggttcggtgc agctcgccga 1321 ccactaccag caaaacactc ctatcgggga cggcccggtt cttctccccg acaaccacta 1381 cctgtcctac cagagcgccc tctccaagga cccaaacgaa aagcgcgatc acatggtctt 1441 gcttgagttc gtgaccgctg ccggaatcac cctgggcatg gacgagcttt acaagtgact 1501 cgtcgaccct gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca 1561 attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt 1621 ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct 1681 ccgttcaatt tactgattgt cctcgacagg ccttaagggc cagatcttgg gcccggtacc 1741 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga 1801 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 1861 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 1921 tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 1981 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 2041 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 2101 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 2161 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 2221 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 2281 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 2341 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt 2401 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 2461 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 2521 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 2581 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 2641 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 2701 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 2761 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 2821 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 2881 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 2941 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 3001 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 3061 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 3121 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 3181 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 3241 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 3301 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 3361 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 3421 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 3481 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 3541 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 3601 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 3661 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 3721 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 3781 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 3841 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 3901 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 3961 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 4021 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 4081 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 4141 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 4201 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 4261 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 4321 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 4381 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 4441 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gt //