LOCUS C971pU10Tnos-U10 6394 bp DNA circular SYN 26-AUG-2020 DEFINITION Ligation of NosU10-Spe-Mlu* into 204pUbiAtmu-ocs* ACCESSION C971pU10Tnos-U10 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 6394) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: 204pUbiAtmu-ocs* Fragment ends: MluI and SpeI Fragment size: 4598 Insert molecule: NosU10-Spe-Mlu* Fragment ends: SpeI and MluI Fragment size: 1796 Primers for gene synthesis Sequencing primers LH1 Sequencing primers NosF3 CCGATCGTTCAAACATTTGGCAATA NosR2 GAGCGATCTAGTAACATAGATGAC FEATURES Location/Qualifiers misc_feature 126..1601 /gene="Pro-Ubq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1197..1200 /product="TATA" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1230..1293 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1296..1599 /product="íntron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1629..1882 /gene="NOS-T" /product="NOS-T" /SECDrawAs="Region" /SECStyleId=1 /SECName="TNOS" /SECDescr="NOS-T" misc_feature 1903..3378 /gene="Pro-Ubq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2974..2977 /product="TATA" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3007..3070 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3073..3376 /product="íntron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3409..3604 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 4420..5280 /gene="Amp" /product="ORF-1" /SECDrawAs="Region" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" misc_feature 5323..6039 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg 121 gccgctttcc ttacattctg agcctctttc cttctaatcc actcatctgc atcttcttgt 181 gtccttacta atacctcatt ggttccaaat tccctccctt taagcaccag ctcgtttctg 241 ttcttccaca gcctcccaag tatccaaggg actaaagcct ccacattctt cagatcagga 301 tattcttgtt taagatgttg aactctatgg aggtttgtat gaactgatga tctaggaccg 361 gataagttcc cttcttcata gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt 421 acattgttat taatgaaaaa atattattgg tcattggact gaacacgagt gttaaatatg 481 gaccaggccc caaataagat ccattgatat atgaattaaa taacaagaat aaatcgagtc 541 accaaaccac ttgccttttt taacgagact tgttcaccaa cttgatacaa aagtcattat 601 cctatgcaaa tcaataatca tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg 661 gataatttca caatatgtta tacgataaag aagttacttt tccaagaaat tcactgattt 721 tataagccca cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc 781 acgaagaatt ctagaaaata cgaaatacgc ttcaatgcag tgggacccac ggttcaatta 841 ttgccaattt tcagctccac cgtatattta aaaaataaaa cgataatgct aaaaaaatat 901 aaatcgtaac gatcgttaaa tctcaacggc tggatcttat gacgaccgtt agaaattgtg 961 gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg 1021 tgtttatcaa ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa 1081 aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca ttttattatt agctattgct 1141 tcaccgcctt agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata 1201 aaacaatacc caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta 1261 aaaactttct ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc 1321 tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga 1381 ttctgttaat cttagatcga agacgatttt ctgggtttga tcgttagata tcatcttaat 1441 tctcgattag ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa 1501 taattactct tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga 1561 tcgaatttgt cgattaatct gagtttttct gattaacagg tactagtctc ggatccctct 1621 ccggacccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg 1681 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca 1741 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca 1801 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg 1861 tgtcatctat gttactagat cgctcgaccc gggagacgcc gctttcctta cattctgagc 1921 ctctttcctt ctaatccact catctgcatc ttcttgtgtc cttactaata cctcattggt 1981 tccaaattcc ctccctttaa gcaccagctc gtttctgttc ttccacagcc tcccaagtat 2041 ccaagggact aaagcctcca cattcttcag atcaggatat tcttgtttaa gatgttgaac 2101 tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt cttcatagcg 2161 aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa tgaaaaaata 2221 ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa ataagatcca 2281 ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg ccttttttaa 2341 cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca ataatcatac 2401 aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa tatgttatac 2461 gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt gcattagata 2521 aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta gaaaatacga 2581 aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca gctccaccgt 2641 atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat cgttaaatct 2701 caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt cagtaataaa 2761 cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa 2821 tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg tgtaaacaac 2881 gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc tttctcgtga 2941 cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa agagctcttc 3001 ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc aattctctct 3061 accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga ttttgttttc 3121 gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt agatcgaaga 3181 cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt ttcatagata 3241 tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg atttgtgatt 3301 tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga ttaatctgag 3361 tttttctgat taacaggtac tgcaggacaa gcttcacacg cgtgtcgacc ctgctttaat 3421 gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg 3481 taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga 3541 atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt 3601 gtcctcgaca ggccttaagg gccagatctt gggcccggta cccaattcgc cctatagtga 3661 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt 3721 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga 3781 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta 3841 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 3901 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 3961 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 4021 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 4081 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 4141 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 4201 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 4261 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga 4321 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 4381 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 4441 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 4501 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 4561 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 4621 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 4681 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 4741 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 4801 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 4861 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 4921 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 4981 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 5041 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 5101 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 5161 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 5221 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 5281 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 5341 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 5401 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 5461 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 5521 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 5581 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 5641 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 5701 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 5761 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 5821 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 5881 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 5941 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 6001 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 6061 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 6121 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 6181 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 6241 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 6301 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag 6361 gctttacact ttatgcttcc ggctcgtatg ttgt //