LOCUS C972pUZmTnos-UZm 5262 bp DNA circular SYN 26-AUG-2020 DEFINITION Ligation of B656p6oA-UbiZ-UbiZ* into pd2AFN* ACCESSION C972pUZmTnos-UZm KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5262) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pd2AFN* Fragment ends: SfiI Fragment size: 1626 Insert molecule: B656p6oA-UbiZ-UbiZ* Fragment ends: SfiI Fragment size: 3636 Sequencing primers: PBRR CTCTGACTTGAGCGTCGATTT RK1 CGACACGGAAATGTTGAATACT FEATURES Location/Qualifiers misc_feature 31..1538 /gene="PUbi" /product="Ubi-int Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1017..1536 /product="Intron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1569..1764 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 1794..3301 /gene="PUbi" /product="Ubi-int Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2780..3299 /product="Intron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3339..3592 /gene="TNOS" /product="NOS-T" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(3681..4345) /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 misc_feature complement(4346..5208) /gene="Amp" /product="Amp-DCS1" /SECDrawAs="Region" /SECStyleId=1 ORIGIN 1 ggccttgggg gccaatttaa atggcgtacg tgcagtgcag cgtgacccgg tcgtgcccct 61 ctctagagat aatgagcatt gcatgtctaa gttataaaaa attaccacat attttttttg 121 tcacacttgt ttgaagtgca gtttatctat ctttatacat atatttaaac tttactctac 181 gaataatata atctatagta ctacaataat atcagtgttt tagagaatca tataaatgaa 241 cagttagaca tggtctaaag gacaattgag tattttgaca acaggactct acagttttat 301 ctttttagtg tgcatgtgtt ctcctttttt tttgcaaata gcttcaccta tataatactt 361 catccatttt attagtacat ccatttaggg tttagggtta atggttttta tagactaatt 421 tttttagtac atctatttta ttctatttta gcctctaaat taagaaaact aaaactctat 481 tttagttttt ttatttaata atttagatat aaaatagaat aaaataaagt gactaaaaat 541 taaacaaata ccctttaaga aattaaaaaa actaaggaaa catttttctt gtttcgagta 601 gataatgcca gcctgttaaa cgccgtcgat cgacgagtct aacggacacc aaccagcgaa 661 ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 721 ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 781 ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 841 gcaccggcag ctacggggga ttcctttccc accgctcctt cgctttccct tcctcgcccg 901 ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg 961 cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac 1021 gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt ccggtccatg 1081 gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga 1141 tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct 1201 aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg 1261 atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc 1321 atatgcagca tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt 1381 atgttttata attattttga tcttgatata cttggatgat ggcatatgca gcagctatat 1441 gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt 1501 cgatgctcac cctgttgttt ggtgttactt ctgcaggtac tagtggatcc cccgggttgg 1561 cgcgccgacc ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt 1621 caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg 1681 gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt 1741 ctccgttcaa tttactgatt gtcctcgacg cggtgtacag tgaagcttgt acgtgcagtg 1801 cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 1861 aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 1921 catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 1981 ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 2041 acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 2101 atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 2161 ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 2221 aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 2281 aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 2341 aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gatcgacgag 2401 tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc 2461 acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac cgttggactt 2521 gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc cggcacggca 2581 ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt cccaccgctc 2641 cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca ccctctttcc 2701 ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca aatccacccg 2761 tcggcacctc cgcttcaagg tacgccgctc gtcctccccc cccccccctc tctaccttct 2821 ctagatcggc gttccggtcc atggttaggg cccggtagtt ctacttctgt tcatgtttgt 2881 gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt cgtacacgga tgcgacctgt 2941 acgtcagaca cgttctgatt gctaacttgc cagtgtttct ctttggggaa tcctgggatg 3001 gctctagccg ttccgcagac gggatcgatc taggataggt atacatgttg atgtgggttt 3061 tactgatgca tatacatgat ggcatatgca gcatctattc atatgctcta accttgagta 3121 cctatctatt ataataaaca agtatgtttt ataattattt tgatcttgat atacttggat 3181 gatggcatat gcagcagcta tatgtggatt tttttagccc tgccttcata cgctatttat 3241 ttgcttggta ctgtttcttt tgtcgatgct caccctgttg tttggtgtta cttctgcagg 3301 tacacctagg cgacgcgtgc gtcgactcga atttccccga tcgttcaaac atttggcaat 3361 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt 3421 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg 3481 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc 3541 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgctcgacgc 3601 atgctggtac ctgacgtctg cggccgcatt aattaaggcc ctaagggcct gatctgtata 3661 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 3721 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 3781 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 3841 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 3901 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 3961 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4021 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4081 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 4141 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 4201 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 4261 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 4321 tcacgttaag ggattttggc catgagatca ccaatgctta atcagtgagg cacctatctc 4381 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4441 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4501 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4561 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 4621 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgctggca tcgtggtgtc 4681 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 4741 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 4801 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 4861 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 4921 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc 4981 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 5041 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 5101 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 5161 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatgg ctgacctcct 5221 atgcagatat cttatgagtg actagaatag ctctcaagat ca //