LOCUS D323p35s-LmVenus 4363 bp DNA circular SYN 25-AUG-2020 DEFINITION Ligation of mVenus-Mlu-Sal* into B629p35s-LGFP* ACCESSION D323p35s-LmVenus KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4363) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B629p35s-LGFP* Fragment ends: SalI and MluI Fragment size: 3580 Insert molecule: mVenus-Mlu-Sal* Fragment ends: MluI and SalI Fragment size: 783 Genesynthesis Sequencing primers: 35RT CTTCGCAAGACCCTTCCTCT FEATURES Location/Qualifiers misc_feature 106..528 /gene="35S-Pro" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" CDS 494..500 /gene="TATA" /product="TATA-box" /translation="YI" /SECDrawAs="Region" /SECStyleId=1 /SECDescr="TATA-box" CDS 627..1346 /gene="ORF-1" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKN GIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH MVLLEFVTAAGITLGMDELYK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="mVenus" misc_feature 1352..1547 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2363..3223 /gene="Amp" /product="ORF-1" /SECDrawAs="Region" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" misc_feature 3266..3982 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagatcaac atggtggagc 121 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 181 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 241 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 301 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 361 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 421 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 481 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacta gttggatccc 541 ccgggctgca ggaattcaag cttacgcgtt gtacaggagt accgccccgt ccggtcctgc 601 ccgtcaccga gatcagcgga gagttcatgg tgtccaaggg cgaggagctg ttcaccggag 661 tcgttcccat cctcgtggaa ctcgacggtg acgtgaacgg acacaagttc agcgtgtctg 721 gggagggaga gggcgatgcc acttacggca agctcaccct taagctgatt tgcaccacag 781 gaaagctccc agtcccttgg ccgaccttgg ttaccactct tggctacgga ctccagtgct 841 tcgctcgcta ccccgaccac atgaagcaac acgacttctt caagtccgcc atgccagagg 901 gttacgtgca ggagaggacc atcttcttta aggacgatgg caactacaag acccgtgcag 961 aagtcaagtt cgagggcgac acgctggtga acagaatcga gctgaagggg atcgacttca 1021 aggaggacgg aaatattctc ggccacaagt tggagtacaa ctacaactca cataacgtct 1081 acatcaccgc cgacaagcag aagaacggaa tcaaagcgaa cttcaagatc cgtcacaata 1141 ttgaggatgg cggtgtgcag ctcgccgacc actatcaaca gaacacccct atcggcgacg 1201 ggccggttct tctccccgac aaccactacc tgtcgtacca gagcaagctc tccaaagatc 1261 caaacgaaaa gagggaccac atggtcttgc ttgagttcgt gactgctgcc ggaatcaccc 1321 tgggcatgga cgagctttac aagtgagtcg accctgcttt aatgagatat gcgagacgcc 1381 tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg 1441 tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact 1501 atcgtatttt tatgaataat attctccgtt caatttactg attgtcctcg acaggcctta 1561 agggccagat cttgggcccg gtacccaatt cgccctatag tgagtcgtat tacaattcac 1621 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 1681 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 1741 cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt gtaagcgtta atattttgtt 1801 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg 1861 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg 1921 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta 1981 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg 2041 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa 2101 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct 2161 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 2221 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 2281 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 2341 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 2401 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 2461 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 2521 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 2581 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 2641 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 2701 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 2761 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 2821 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 2881 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 2941 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 3001 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 3061 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 3121 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 3181 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 3241 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 3301 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 3361 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 3421 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 3481 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 3541 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 3601 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 3661 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 3721 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 3781 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 3841 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 3901 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 3961 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 4021 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 4081 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 4141 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 4201 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 4261 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 4321 tccggctcgt atgttgtgtg gaattgtgag cggataacaa ttt //