LOCUS D894p35s-LmEGFPo 4364 bp DNA circular SYN 26-AUG-2020 DEFINITION 35s-LmEGFPoi Circularized ACCESSION D894p35s-LmEGFPo KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4364) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Primers for gene synthesis GFPoiF GTATCGATTTCAAGGAAGATGGA GFPoiR CTTTCAGCTCGATGCG Sequencing primers: M13RY CACACAGGAAACAGCTATGAC GFP-F GACTATCTTCTTCAAGGATGAC FEATURES Location/Qualifiers misc_feature 106..528 /gene="35S-Pro" /product="Promotor-Region" /SECDrawAs="Region" /SECStyleId=1 /SECName="P35S" /SECDescr="Promotor-Region" CDS 494..500 /gene="TATA" /product="TATA-box" /translation="YI" /SECDrawAs="Region" /SECStyleId=1 /SECDescr="TATA-box" CDS 627..1343 /gene="ORF-3" /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHM VLLEFVTAAGITHGMDELYK" /SECDrawAs="Gene" /SECStyleId=1 /SECName="mEGFP" misc_feature 816..821 /product="F64L/S65T - enhanced fluorescence" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1242..1244 /product="A206K - monomeric protein" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1353..1548 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2364..3224 /gene="Amp" /product="ORF-1" /SECDrawAs="Region" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" misc_feature 3267..3983 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagatcaac atggtggagc 121 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta 181 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta 241 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt 301 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac 361 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 421 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 481 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacta gttggatccc 541 ccgggctgca ggaattcaag cttacgcgtt gtacaggagt accgccccgt ccggtcctgc 601 ccgtcaccga gatcagcgga gagttcatgg gcaagggcga ggaactgttc actggcgtgg 661 tcccaatcct ggtggaactg gatggtgatg tgaacgggca caagttctcc gtcagcggag 721 agggtgaagg tgatgccacc tacggaaagc tcaccctgaa gttcatctgc actaccggaa 781 agctccctgt tccgtggcca accctcgtca ccactttcac ctacggtgtt cagtgcttct 841 cccgttaccc agatcacatg aagcagcatg acttcttcaa gagcgccatg cccgaaggct 901 acgtgcaaga aaggactatc ttcttcaagg atgacgggaa ctacaagaca cgtgccgaag 961 tcaagttcga aggtgatacc ctggtgaacc gcatcgagct gaaaggtatc gatttcaagg 1021 aagatggaaa catcctcgga cacaagctgg agtacaacta caactcccac aacgtataca 1081 tcatggccga caagcagaag aacggcatca aggtgaactt caagatcagg cacaacatcg 1141 aagatggaag cgtgcaactg gcggaccact accagcagaa cacgcccatc ggcgatggcc 1201 ctgtcctgct gccggacaac cattacctgt ccacgcaatc taagctctcc aaggacccca 1261 acgagaagag ggaccacatg gtcctgctgg agttcgtgac ggctgctggg atcacgcatg 1321 gcatggatga actctacaag tgacccggtc gaccctgctt taatgagata tgcgagacgc 1381 ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat 1441 gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat cacccgttac 1501 tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtcctc gacaggcctt 1561 aagggccaga tcttgggccc ggtacccaat tcgccctata gtgagtcgta ttacaattca 1621 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 1681 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 1741 ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt aatattttgt 1801 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 1861 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 1921 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 1981 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 2041 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 2101 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 2161 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 2221 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 2281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 2341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 2401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 2461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 2521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 2581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 2641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 2701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 2761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 2821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 2881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 2941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 3001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 3061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 3121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 3181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 3241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 3301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 3361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 3421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 3481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 3541 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 3601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 3661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 3721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 3781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 3841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 3901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 3961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4021 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 4081 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 4141 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 4201 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 4261 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 4321 ttccggctcg tatgttgtgt ggaattgtga gcggataaca attt //